220 research outputs found
Ecological network construction based on minimum cumulative resistance for the City of Nanjing, China
With economic growth and the improvement of the urbanization level, human activities have constantly interfered with landscape patterns, resulting in serious threats to regional ecological security. Therefore, it is of great significance to study the evolution and optimization of the landscape patterns. Based on three TM images from 1990, 2000, and 2010, and selected landscape pattern indexes, the changes in the landscape pattern of Nanjing in the past twenty years were studied based on landscape ecology theory using Remote Sensing (RS) and a Geographical Information System (GIS). The ecological network was built on the basis of extracted ecological nodes and the minimum cumulative resistance. The results show that changes in the landscape pattern of the city of Nanjing were notable. Class-level indexes indicate that the farmland landscape area decreased and the degree of patch fragmentation increased. The construction land area increased, and it tended to show dispersed distribution. The proportion of forest land increased and the shape of patches became more complex. The proportion of water firstly showed a decrease, followed by an increase, and the shape of the water became more regular. Landscape-level indexes indicate that biological diversity and the degree of fragmentation increased. Spatial heterogeneity of the natural landscape increased, and the patch shape of each landscape type developed similarly. The results also call for stepping-stones to enhance the connectivity and optimization of the ecological network, which will help improve ecological services and improve the landscape pattern of the city
Prediction of protein-protein interaction sites using an ensemble method
<p>Abstract</p> <p>Background</p> <p>Prediction of protein-protein interaction sites is one of the most challenging and intriguing problems in the field of computational biology. Although much progress has been achieved by using various machine learning methods and a variety of available features, the problem is still far from being solved.</p> <p>Results</p> <p>In this paper, an ensemble method is proposed, which combines bootstrap resampling technique, SVM-based fusion classifiers and weighted voting strategy, to overcome the imbalanced problem and effectively utilize a wide variety of features. We evaluate the ensemble classifier using a dataset extracted from 99 polypeptide chains with 10-fold cross validation, and get a AUC score of 0.86, with a sensitivity of 0.76 and a specificity of 0.78, which are better than that of the existing methods. To improve the usefulness of the proposed method, two special ensemble classifiers are designed to handle the cases of missing homologues and structural information respectively, and the performance is still encouraging. The robustness of the ensemble method is also evaluated by effectively classifying interaction sites from surface residues as well as from all residues in proteins. Moreover, we demonstrate the applicability of the proposed method to identify interaction sites from the non-structural proteins (NS) of the influenza A virus, which may be utilized as potential drug target sites.</p> <p>Conclusion</p> <p>Our experimental results show that the ensemble classifiers are quite effective in predicting protein interaction sites. The Sub-EnClassifiers with resampling technique can alleviate the imbalanced problem and the combination of Sub-EnClassifiers with a wide variety of feature groups can significantly improve prediction performance.</p
Aerobic Copper-Promoted Oxidative Dehydrosulfurative Carbon-Oxygen Cross-Coupling of 3,4-Dihydropyrimidine-1: H -2-Thiones With Alcohols
An aerobic Cu-promoted oxidative dehydrosulfurative carbon-oxygen cross-coupling of 3,4-dihydropyrimidin-1H-2-thiones (DHPMs) with both aliphatic and aromatic alcohols is described. Together with the ready availability of DHPMs and both alcohols, the method furnishes facile access to biologically valuable 2-alkoxypyrimidines with rapid diversification
Improving transferability of 3D adversarial attacks with scale and shear transformations
Previous work has shown that 3D point cloud classifiers can be vulnerable to
adversarial examples. However, most of the existing methods are aimed at
white-box attacks, where the parameters and other information of the
classifiers are known in the attack, which is unrealistic for real-world
applications. In order to improve the attack performance of the black-box
classifiers, the research community generally uses the transfer-based black-box
attack. However, the transferability of current 3D attacks is still relatively
low. To this end, this paper proposes Scale and Shear (SS) Attack to generate
3D adversarial examples with strong transferability. Specifically, we randomly
scale or shear the input point cloud, so that the attack will not overfit the
white-box model, thereby improving the transferability of the attack. Extensive
experiments show that the SS attack proposed in this paper can be seamlessly
combined with the existing state-of-the-art (SOTA) 3D point cloud attack
methods to form more powerful attack methods, and the SS attack improves the
transferability over 3.6 times compare to the baseline. Moreover, while
substantially outperforming the baseline methods, the SS attack achieves SOTA
transferability under various defenses. Our code will be available online at
https://github.com/cuge1995/SS-attackComment: 10 page
Does Increasing Model Resolution Improve the Real-Time Forecasts of Western North Pacific Tropical Cyclones?
In this study, the general impact of high-resolution moving nesting domains on tropical cyclone (TC) intensity and track forecasts was verified, for a total of 107 forecast cases of 33 TCs, using the Weather Research and Forecasting (WRF) model. The experiment, with a coarse resolution of 12 km, could not significantly capture the intensification process, especially for maximum intensities (>60 m s(-1)). The intense TCs were better predicted by experiments using a moving nesting domain with a horizontal resolution of 4 km. The forecast errors for maximum wind speed and minimum sea-level pressure decreased in the experiment with higher resolution; the forecast of lifetime maximum intensity was improved. For the track forecast, the experiment with a coarser resolution tended to simulate TC tracks deviating rightward to the TC motions in the best-track data; this erroneous deflection was reduced in the experiment with a higher resolution. In particular, the track forecast in the experiment with a higher resolution improved more frequently for intense TCs that were generally distributed at relatively lower latitudes among the test cases. The sensitivity of the track forecast to the model resolution was relatively significant for lower-latitude TCs. On the other hand, the track forecasts of TCs moving to the mid-latitudes, which were primarily influenced by large-scale features, were not sensitive to the resolution
Synaptic Vesicles Position Complexin to Block Spontaneous Fusion
SummarySynapses continually replenish their synaptic vesicle (SV) pools while suppressing spontaneous fusion events, thus maintaining a high dynamic range in response to physiological stimuli. The presynaptic protein complexin can both promote and inhibit fusion through interactions between its α-helical domain and the SNARE complex. In addition, complexin’s C-terminal half is required for the inhibition of spontaneous fusion in worm, fly, and mouse, although the molecular mechanism remains unexplained. We show here that complexin’s C-terminal domain binds lipids through a novel protein motif, permitting complexin to inhibit spontaneous exocytosis in vivo by targeting complexin to SVs. We propose that the SV pool serves as a platform to sequester and position complexin where it can intercept the rapidly assembling SNAREs and control the rate of spontaneous fusion
The Tianlin Mission: a 6m UV/Opt/IR space telescope to explore the habitable worlds and the universe
[Abridged] It is expected that the ongoing and future space-borne planet
survey missions including TESS, PLATO, and Earth 2.0 will detect thousands of
small to medium-sized planets via the transit technique, including over a
hundred habitable terrestrial rocky planets. To conduct a detailed study of
these terrestrial planets, particularly the cool ones with wide orbits, the
exoplanet community has proposed various follow-up missions. The currently
proposed ESA mission ARIEL is capable of characterization of planets down to
warm super-Earths mainly using transmission spectroscopy. The NASA 6m
UV/Opt/NIR mission proposed in the Astro2020 Decadal Survey may further tackle
down to habitable rocky planets, and is expected to launch around 2045. In the
meanwhile, China is funding a concept study of a 6-m class space telescope
named Tianlin (A UV/Opt/NIR Large Aperture Space Telescope) that aims to start
its operation within the next 10-15 years and last for 5+ years. Tianlin will
be primarily aimed to the discovery and characterization of rocky planets in
the habitable zones (HZ) around nearby stars and to search for potential
biosignatures mainly using the direct imaging method. Transmission and emission
spectroscopy at moderate to high resolution will be carried out as well on a
population of exoplanets to strengthen the understanding of the formation and
evolution of exoplanets. It will also carry out in-depth studies of the cosmic
web and early galaxies, and constrain the nature of the dark matter and dark
energy. We describe briefly the primary scientific motivations and main
technical considerations based on our preliminary simulation results. We find
that a monolithic off-axis space telescope with a primary mirror diameter
larger than 6m equipped with a high contrast chronograph can identify water in
the atmosphere of a habitable-zone Earth-like planet around a Sun-like star.Comment: 15 pages, 5 figures, accepted for publication in RAA and is available
onlin
Degradation of difenoconazole in water and soil: Kinetics, degradation pathways, transformation products identification and ecotoxicity assessment
Difenoconazole is a widely used triazole fungicide that has been frequently detected in the environment, but comprehensive study about its environmental fate and toxicity of potential transformation products (TPs) is still lacking. Here, laboratory experiments were conducted to investigate the degradation kinetics, pathways, and toxicity of transformation products of difenoconazole. 12, 4 and 4 TPs generated by photolysis, hydrolysis and soil degradation were identified via UHPLC-QTOF/MS and the UNIFI software. Four intermediates TP295, TP295A, TP354A and TP387A reported for the first time were confirmed by purchase or synthesis of their standards, and they were further quantified using UHPLC-MS/MS in all tested samples. The main transformation reactions observed for difenoconazole were oxidation, dechlorination and hydroxylation in the environment. ECOSAR prediction and laboratory tests showed that the acute toxicities of four novel TPs on Brachydanio rerio, Daphnia magna and Selenastrum capricornutum are substantially lower than that of difenoconazole, while all the TPs except for TP277C were predicted chronically very toxic to fish, which may pose a potential threat to aquatic ecosystems. The results are important for elucidating the environmental fate of difenoconazole and assessing the environmental risks, and further provide guidance for scientific and reasonable use.acceptedVersio
A nomogram based on CT intratumoral and peritumoral radiomics features preoperatively predicts poorly differentiated invasive pulmonary adenocarcinoma manifesting as subsolid or solid lesions: a double-center study
BackgroundThe novel International Association for the Study of Lung Cancer (IASLC) grading system suggests that poorly differentiated invasive pulmonary adenocarcinoma (IPA) has a worse prognosis. Therefore, prediction of poorly differentiated IPA before treatment can provide an essential reference for therapeutic modality and personalized follow-up strategy. This study intended to train a nomogram based on CT intratumoral and peritumoral radiomics features combined with clinical semantic features, which predicted poorly differentiated IPA and was tested in independent data cohorts regarding models’ generalization ability.MethodsWe retrospectively recruited 480 patients with IPA appearing as subsolid or solid lesions, confirmed by surgical pathology from two medical centers and collected their CT images and clinical information. Patients from the first center (n =363) were randomly assigned to the development cohort (n = 254) and internal testing cohort (n = 109) in a 7:3 ratio; patients (n = 117) from the second center served as the external testing cohort. Feature selection was performed by univariate analysis, multivariate analysis, Spearman correlation analysis, minimum redundancy maximum relevance, and least absolute shrinkage and selection operator. The area under the receiver operating characteristic curve (AUC) was calculated to evaluate the model performance.ResultsThe AUCs of the combined model based on intratumoral and peritumoral radiomics signatures in internal testing cohort and external testing cohort were 0.906 and 0.886, respectively. The AUCs of the nomogram that integrated clinical semantic features and combined radiomics signatures in internal testing cohort and external testing cohort were 0.921 and 0.887, respectively. The Delong test showed that the AUCs of the nomogram were significantly higher than that of the clinical semantic model in both the internal testing cohort(0.921 vs 0.789, p< 0.05) and external testing cohort(0.887 vs 0.829, p< 0.05).ConclusionThe nomogram based on CT intratumoral and peritumoral radiomics signatures with clinical semantic features has the potential to predict poorly differentiated IPA manifesting as subsolid or solid lesions preoperatively
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