Ginsenoside induces cell death in breast cancer cells via ROS/PI3K/Akt signaling pathway

Purpose: To study the influence of ginsenoside on breast carcinoma, and the mechanism of action involved.Methods: Different concentrations of ginsenoside were used to treat MCF-7 breast cancer cell line. Cell viability was measured by MTT assay, while protein expressions of p-Akt and p-PI3K were determined using Western blotting. The concentrations of reactive oxidative reactants and reactive oxygen species (ROS) were assessed using fluorescence immunoassay and immunofluorescence assay. The mechanism of action involved in ginsenoside-mediated apoptosis was determined based on ROS/PI3K/Akt signaling pathway.Results: There was no change in the inhibition of MCF-7 cell proliferation in control cells with time (p > 0.05). However, inhibition of MCF-7 cell proliferation in ginsenoside group was significantly higher than that in the control group (p < 0.05); furthermore, it increased with time and ginsenoside concentration. Apoptosis was markedly and concentration-dependently higher in ginsenoside-treated MCF-7 cells than in controls (p > 0.05). There were lower protein levels of p-PI3K and p-Akt in ginsenoside-exposed MCF-7 cells than in control group; the protein expressions  decreased with increase in ginsenoside concentration (p < 0.05). The expressions of ROS in ginsenoside-treated MCF-7 cells declined, relative to the untreated group; in addition, the expressions decreased with increase in ginsenoside concentration (p < 0.05).Conclusion: Ginsenoside suppresses proliferation of MCF-7 cell line, and exerts apoptotic effect on the cells via inhibition of the ROS/PI3K/Akt signal pathway. This provides a new approach to treat breast cancer. Keywords: Breast cancer cells, Ginsenoside, Apoptosis, ROS/PI3K/Akt signaling pathwa

Glycyrrhizin inhibits the invasion and metastasis of breast cancer cells via upregulation of expressions of miR-200c and e-cadherin

Purpose: To determine the inhibitory effect of glycyrrhizin (GLA) on cell invasion and metastasis in mammary carcinoma cells, and the mechanisms of actions involved.Methods: The effect of GLA at different concentrations on proliferation of breast cancer MDA-MB-231 and BT549 cells was assayed by MTT method. Transwell assay was used to determine the effect of GLA at different concentrations on invasiveness and metastasis of breast cancer MDA-MB-231 and BT549 cells. The influence of LGA on expressions of microRNA-200c and miR-200c was assayed by reverse transcriptase-polymerase chain reaction (RT-PCR).Results: There was no statistically significant difference in cell proliferation amongst cells treated with 5 and 20 μM GLA and untreated breast cancer cells. However, the proliferation of cells treated with 40 μM GLA was significantly reduced (p < 0.05). In the cell invasion and migration experiments, cell population transferred to the base of Transwell chamber in the two cell lines treated with GLA was markedly decreased, relative to cells without GLA treatment, while the number of cells decreased with increase in GLA concentration (p < 0.05). Results from image-pro-plus analysis revealed that the population of cells quantitatively crossing the Transwell compartment membrane decreased with increase in GLA concentration (p < 0.05). The expression of e-cadherin was increased by GLA treatment in a concentration-dependent manner. Moreover, GLA treatment led to significant changes in amounts of miR-200s a, b and c, with changes in miR-200c being the most significant (p < 0.05).Conclusion: GLA suppresses the invasiveness and metastasis of breast cancer MDA-MB-231 and BT549 cells via upregulation of the expressions of miR-200c and e-cadherin. These findings provide a theoretical basis for the development of new breast cancer drugs. Keywords: Glycyrrhiza, GLA, miR-200c, E-cadherin, Inhibition, Breast cancer cells, Invasion, Metastasi

Optimization of Stakeholder Relation Network of the Qingdao Elderly Livable Community Construction Project

Due to the population ageing, building an elderly livable community has become an urgent task of social welfare development. This Public-Private Partnership construction project faces a variety of pressures from its complex stakeholders. Based on the Qingdao elderly livable community construction project, this paper builds up interpretations about its relationship governance by conducting stakeholder analysis. The paper aims to explore the relationship governance mechanism of multiple connections between related stakeholders. On the basis of complex network theory, this paper establishes a stakeholder relationship network model and describes different modes of different stakeholder relationship in the Qingdao construction project. The paper analyzes the optimal decision-making behavior and interaction of different stakeholders, constructs the objective functions of stakeholder relationship network, applies centrality measure and dominant-set clustering to analyze the optimal conditions of the whole network, and finally carries out simulation calculation. The results show that it is feasible and effective to apply network analysis method to the study of stakeholder relationship in Public-Private Partnership construction projects

A Framework of Fingerprint Scaling

Fingerprint scaling refers to the adjustment of fingerprint images to solve the problem of sensor interoperability. In this paper, we present an innovative framework of fingerprint scaling with minimum modification to existing systems. For the purpose of facilitating system configuration, we have developed a series of scaling methods, including scaling factors, graph- and template-based scaling methods. In graph-based scaling methods, we have explored the application of various technologies in estimation of the average inter-ridge distance. In template-based scaling methods, we have developed an estimation method using Delaunay triangulation algorithm. The experiments show that the performance achieved by using this framework is better than that of original system. With a scaling module, the average EER in our experiments drops from 27.78% to 13.89%. DOI: http://dx.doi.org/10.11591/telkomnika.v11i3.230

The complete mitochondrial genome of Turdus obscurus (Passeriformes: Turdidae)

The Eyebrowed Thrush (Turdus obscurus) is a highly migratory bird, which breeds in northeastern Asia and overwinters in southeastern Asia. We obtained the mitochondrial genome of T. obscurus by Sanger sequencing. The mitogenome was 16,739 bp in length, which contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region. Its composition is consistent with the species in genus Turdus. Phylogenetic analysis based on the whole mitochondrial genome showed that the relationship between T. obscurus and Turdus kessleri was relatively close. This study improves the understanding of phylogeny and genetics of Turdidae and Muscicapoidea

Double Deep Q-Network with Dynamic Bootstrapping for Real-Time Isolated Signal Control: A Traffic Engineering Perspective

Real-time isolated signal control (RISC) at an intersection is of interest in the field of traffic engineering. Energizing RISC with reinforcement learning (RL) is feasible and necessary. Previous studies paid less attention to traffic engineering considerations and under-utilized traffic expertise to construct RL tasks. This study profiles the single-ring RISC problem from the perspective of traffic engineers, and improves a prevailing RL method for solving it. By qualitative applicability analysis, we choose double deep Q-network (DDQN) as the basic method. A single agent is deployed for an intersection. Reward is defined with vehicle departures to properly encourage and punish the agent’s behavior. The action is to determine the remaining green time for the current vehicle phase. State is represented in a grid-based mode. To update action values in time-varying environments, we present a temporal-difference algorithm TD(Dyn) to perform dynamic bootstrapping with the variable interval between actions selected. To accelerate training, we propose a data augmentation based on intersection symmetry. Our improved DDQN, termed D3ynQN, is subject to the signal timing constraints in engineering. The experiments at a close-to-reality intersection indicate that, by means of D3ynQN and non-delay-based reward, the agent acquires useful knowledge to significantly outperform a fully-actuated control technique in reducing average vehicle delay

HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2

Large quantities of long noncoding RNAs (lncRNAs) have been verified to exert vital functions in the process of breast cancer (BC). lncRNA heart and neural crest derivatives expressed 2-antisense RNA 1 (HAND2-AS1) was reported to suppress the development of several cancers. However, its detailed function in BC remained unclear. In the current study, HAND2-AS1 was discovered to be low expressed in BC cell lines, and overexpression of HAND2-AS1 could repress proliferation, migration, and invasion but facilitate apoptosis in BC cells. Moreover, HAND2-AS1 was found to act as a sponge of miR-3118 which was detected to be upregulated in BC cell lines. miR-3118 depletion could constrict the progression of BC. HAND-AS1 hindered the course of BC by reducing the expression of miR-3118. Besides, PHLPP2 was treated as a downstream target of miR-3118 under the selection of RNA pull-down assays. HAND2-AS1 inhibited the process of BC by enhancing expression of PHLPP2. In summary, our study testified that HAND2-AS1 suppressed BC growth by targeting the miR-3118/PHLPP2 axis, indicating that HAND2-AS1 could be regarded as a potential target for BC treatment

The comparison of maintenance treatment with capecitabine (CMT) and non-maintenance treatment with capecitabine (non-CMT) in patients with metastatic breast cancer

Abstract: Aim: The study examined the response rate, response duration and toxicity of maintenance treatment (CMT) and non-maintenance treatment with capecitabine (non-CMT) in metastatic breast cancer (MBC). Material and methods: Between September 2009 and July 2013, a group of 82 patients with MBC, who had progressed after anthracycline/taxane chemotherapy, was treated with a capecitabine-based chemotherapy and divided into two groups. 54 patients received CMT 1.5 g twice a day from days 1 to 14, and 28 patients achieved non-CMT. Treatment was continued until disease progression or unacceptable toxicity. The median age of patients treated with CMT and non-CMT was 57 years (range 38-78) and 50 years (range 37-77). The evaluation of treatment effect was possible in all patients. Results: The overall response rate (ORR) was 29.7% (16 cases), including 3 (5.6%) complete responses (CR) and 13 (24.1%) partial responses (PR). Stable disease (SD) was observed in 7.4% of patients receiving CMT (54 patients). In the group receiving non-CMT, ORR was 3.6% (1 case). The median PFS in CMT group was 36 weeks, while in non-CMT group was 24 weeks. The most common adverse event was hematologic toxicity (74.1%), with the incidence of grade 1-2/3-4 was 70.4% and 3.7%. Hand-foot syndrome was the most frequent nonhematologic form of toxicity, occurring in 70.4% of cases. There were no treatment-related deaths. Conclusions: CMT is an effective and safe treatment for pretreated metastatic breast cancer patients. And CMT appears to be a more efficacious treatment than non-CMT