788 research outputs found
Three-dimensional microfabrication through a multimode optical fiber
Additive manufacturing, also known as 3D printing, is an advanced
manufacturing technique that allows the fabrication of arbitrary macroscopic
and microscopic objects. All 3D printing systems require large optical elements
or nozzles in proximity to the built structure. This prevents their use in
applications in which there is no direct access to the area where the objects
have to be printed. Here, we demonstrate three-dimensional microfabrication
based on two-photon polymerization (TPP) with sub diffraction-limited
resolution through an ultra-thin, 50 mm long printing nozzle of 560 micrometers
in diameter. Using wavefront shaping, femtosecond infrared pulses are focused
and scanned through a multimode optical fiber (MMF) inside a photoresist that
polymerizes via two-photon absorption. We show the construction of arbitrary 3D
structures of 500 nm resolution on the other side of the fiber. To our
knowledge, this is the first demonstration of microfabrication through a
multimode optical fiber. Our work represents a new area which we refer to as
endofabrication
Leeches (Annelida: Hirudinida) of Northern Arkansas
Twenty-one lotic and lentic environments throughout central and northern Arkansas were surveyed for the presence of leeches during June 2004 and April, July - October 2005. Fourteen species of leeches {Helobdella elongata, Helobdella papillata, Helobdella stagnalis, Placobdella cryptobranchii, Placobdella multilineata, Placobdella ornata, Placobdella papillifera, Placobdella parasitica, Placobdella phaler a, Placobdella picta, Haemopis marmorata, Erpobdella fervida, Erpobdella microstoma, and Erpobdella punctata) representing 3 families were collected. Five species (H. elongata, P. cryptobranchii, P. multilineata, H. marmorata, and E. fervida) are reported from Arkansas for the first time. The natural history of the 22 species of leeches now known from Arkansas is reviewed
Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber
We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x10(13) W/cm(2). sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 mu m in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreemen
Partner-assisted emotional disclosure for patients with gastrointestinal cancer: Results from a randomized controlled trial
For patients with cancer who are married or in an intimate relationship, their relationships with their partners play a critical role in their adaptation to their illness. However, cancer patients and their partners often have difficulty in talking with each other about their cancer-related concerns. Difficulties in communication may ultimately compromise both the patient-partner relationship and the patient's psychological adjustment. The present study tested the efficacy of a novel partner-assisted emotional disclosure intervention in a sample of patients with gastrointestinal (GI) cancer
EC76-219 Nebraska Swine Report
This 1976 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats
EC76-219 Nebraska Swine Report
This 1976 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats
Unconventional human T cells accumulate at the site of infection in response to microbial ligands and induce local tissue remodeling
The antimicrobial responsiveness and function of unconventional human T cells are poorly understood, with only limited access to
relevant specimens from sites of infection. Peritonitis is a common and serious complication in individuals with end-stage kidney
disease receiving peritoneal dialysis. By analyzing local and systemic immune responses in peritoneal dialysis patients presenting
with acute bacterial peritonitis and monitoring individuals before and during defined infectious episodes, our data show that Vg9/
Vd2+ gd T cells and mucosal-associated invariant T cells accumulate at the site of infection with organisms producing (E)-4-
hydroxy-3-methyl-but-2-enyl pyrophosphate and vitamin B2, respectively. Such unconventional human T cells are major producers
of IFN-g and TNF-a in response to these ligands that are shared by many microbial pathogens and affect the cells lining
the peritoneal cavity by triggering local inflammation and inducing tissue remodeling with consequences for peritoneal membrane
integrity. Our data uncover a crucial role for Vg9/Vd2 T cells and mucosal-associated invariant T cells in bacterial infection and
suggest that they represent a useful predictive marker for important clinical outcomes, which may inform future stratification and
patient management. These findings are likely to be applicable to other acute infections where local activation of unconventional
T cells contributes to the antimicrobial inflammatory response
Lensless two-photon imaging through a multicore fiber with coherence-gated digital phase conjugation
- …