Primers used for PCR detection of genes encoding virulence factors.

<p>Other virulence factors have been detected by multiplex PCR onto purified total DNA (Qiagen). Presence of genes encoding enterotoxins E, G, H, K, L, T, epidermolysin D (Etd), genes encoding Edin A and EDIN B and the genes encoding seven adhsesion factors (Cna, FnbA, FnbB Bbp, Clfb, Fib, Ebp and Lbp) was checked in 8 set in function of base size.</p

Pulsed field gel electrophoresis (PFGE) dependent dendogram of isolated <i>Staphylococcus aureus</i>.

<p>Pulsed field gel electrophoresis (PFGE) proves no specific clonal relationship between PVL-producing isolates issued from furuncles or secondary dermatosis. The similarity of the different pulsotypes was established by using Molecular Analyst™ software. Twenty four pulsotypes corresponded to the 55 isolates and their distribution is given according to the groups of isolates issued from secondary infected dermatosis (1), furuncles from HIV (−)(2) or HIV (+)(3) patients.</p

Production of toxins and identification of genes encoding toxins in <i>Staphylococcus aureus</i>.

<p>The majority of <i>SA</i> strains isolated from HIV patient-derived furuncles significantly produced PVL (<i>p</i><0.05), whereas only 10% of <i>SA</i> strains produced this toxin in secondary infected dermatosis. A high prevalence of LukE-LukD-producing isolates (56 to 78%) was recorded in patient groups.</p