Vitamin A, cancer treatment and prevention: The new role of cellular retinol binding proteins

Retinol and vitamin A derivatives influence cell differentiation, proliferation, and apoptosis and play an important physiologic role in a wide range of biological processes. Retinol is obtained from foods of animal origin. Retinol derivatives are fundamental for vision, while retinoic acid is essential for skin and bone growth. Intracellular retinoid bioavailability is regulated by the presence of specific cytoplasmic retinol and retinoic acid binding proteins (CRBPs and CRABPs). CRBP-1, the most diffuse CRBP isoform, is a small 15 KDa cytosolic protein widely expressed and evolutionarily conserved in many tissues. CRBP-1 acts as chaperone and regulates the uptake, subsequent esterification, and bioavailability of retinol. CRBP-1 plays a major role in wound healing and arterial tissue remodelling processes. In the last years, the role of CRBP-1-related retinoid signalling during cancer progression became object of several studies. CRBP-1 downregulation associates with a more malignant phenotype in breast, ovarian, and nasopharyngeal cancers. Reexpression of CRBP-1 increased retinol sensitivity and reduced viability of ovarian cancer cells in vitro. Further studies are needed to explore new therapeutic strategies aimed at restoring CRBP-1-mediated intracellular retinol trafficking and the meaning of CRBP-1 expression in cancer patients' screening for a more personalized and efficacy retinoid therapy

Functional implant prosthodontic score of a one-year prospective study on three different connections for single-implant restorations

Aim The aim of this prospective clinical trial was to analyze, using the Functional Implant Prosthodontic Score (FIPS), the clinical resultsof three different abutment-implant connections (1 hexagon vs 2 conical types) single-unit restorations after one year of clinical service. Material and methods Thirty patients were restored with cement-retained crowns on soft tissue level implants (10 TTc Windmix, 10 TTk Windmix and 10 Aadva GC) in posterior sites and followed-up for 1 year. FIPS was applied for objective outcome assessment beside clinical and radiographic examinations. Five variables were defined for evaluation, resulting in a maximum score of 10 per implant restoration. The patients’ level of satisfaction was recorded and correlated with FIPS. Results All implants and connected crowns revealed survival rates of 100% without any biological or technical complications after three years of loading. The total FIPS recorded for group 1 was 44, 43 in group 2 and 42 in group 3. The mean total FIPS score was 8.6±1.1, ranging from 6 to 10. The variable “bone” revealed the highest scores (2.0; range: 2–2), as well “occlusion” (2.0; range: 2–2). Mean scores for “design” (1.7 ±0.4; range: 1–2), “mucosa” (1.6±0.5; range: 1–2), and “interproximal” (1.5±0.6; range: 1–2) were more challenging to satisfy. The patients expressed a high level of functional satisfaction (80.5±2.5; range: 65–100). No type of connection showed to be superior to the other two. No statistically significant differences were found among the three tested groups. A significant correlation was found between FIPS and the subjective patients’ perception with a coefficient of 0.80 (P < 0.0001). Conclusions The findings of the clinical trial indicated the great potential of both conical and hexagon connections and their good performance after 1 year of clinical service. FIPS showed to be an objective and reliable instrument to assess implant success

A new generation of orthodontic retainer using 3D printing technology: report of two cases

Aim In this article the fabrication and use of new type 3D printed splint of retainer after orthodontic treatments is reported. Case report Two cases, one of an adoescent female patient and the other of an adult female, are presented, describing step-by-step the clinical and laboratory procedures. The controls after 6 months are also reported. Conclusion Further randomized clinical trials are required in order to evaluate durability and efficacy and periodontal parameters in patients treated with this new type of retainer

Biological and predictive role of ERCC1 polymorphisms in cancer

Excision repair cross-complementation group 1 (ERCC1) is a key component in DNA repair mechanisms and may influence the tumor DNA-targeting effect of the chemotherapeutic agent oxaliplatin. Germline ERCC1 polymorphisms may alter the protein expression and published data on their predictive and prognostic value have so far been contradictory. In the present article we review available evidence on the clinical role and utility of ERCC1 polymorphisms and, in the absence of a ‘perfect’ trial, what we call the ‘sliding doors’ trial, we present the data of ERCC1 genotyping in our local patient population. We found a useful predictive value for oxaliplatin-induced risk of anemia

Low-grade fibromyxoid sarcoma: an unusual cardiac location

We report the unusual cardiac localization of a primary low-grade fibromyxoid sarcoma of the right ventricle in a 57-year-old woman. Histological examination revealed a prevalent myxoid appearance with whorling growth pattern of small or spindle cells with bland features alternating with rare more collagenous hypocellular areas with rare atypical cells. Genomic polymerase chain reaction of genomic DNA revealed the typical FUS/Creb3L2 fusion gene products typical of low-grade fibromyxoid sarcoma. The tumor was surgically removed and recurred after 7 years as high-grade pleomorphic sarcoma. The patient died 6 months after the clinical manifestation of recurrence. Low-grade fibromyxoid sarcoma of soft tissues is a rare, distinctive variant of fibrosarcoma-typically arising in deep soft tissue of lower extremities and trunk-that rarely metastasizes. Clinically, low-grade fibromyxoid sarcoma is characterized by a longer survival rate compared to other sarcomas, suggesting its consideration in the differential diagnosis of cardiac tumors with a myxoid appearance

Distal movement of maxillary canines and premolars with sectional mechanics following Distal Jet application to molars. Utilizzo dei sezionali di arretramento per premolari e canini superiori dopo la distalizzazione molare con Distal Jet.

Objectives: to assess the effectiveness of Distal Jet without simultaneous fixed appliance treatment. Subjects and methods: twenty subjects with Class II malocclusion who received the Distal Jet appliance to move maxillary molars distally. Subsequently,the canine and premolar distal movements were achieved by the use of .017 x .025 Ni-Ti sectional wires and memory power chain. Results: revealed that Distal Jet is more effective with less anchorage loss when this appliance is used independent of multibracket appliances. Conclusion: this study showed that Distal Jet is an effective and predictable appliance. The distal movements of upper premolars, canines and incisors with sectional wires fabricated from .017 x .025 Ni-Ti were biomechanically very effective, simple in clinical application, and did not require any patient compliance

Failure evaluation after a 6-year retention period: a comparison between glass fiber-reinforced (GFR) and multistranded bonded retainers. [Évaluation des échecs après six ans de contention : comparaison des appareils de contention en composite renforcé aux fibres (CRF) et les fils torsades collés]

Objectives: The aim of this study was to compare bond failure and breakage rates of two types of bonded lingual orthodontic retainers (GFR and .0175" multistranded stainless steel wire [MST]) after a 6-year retention period. Subjects and methods: Eighty-five young adults were randomly divided into two groups: 40 subjects received 48 GFR retainers (14 maxillary and 34 mandibular retainers), and 45 subjects received 50 MST retainers (18 maxillary and 32 mandibular retainers). A Fisher's exact test was performed in order to identify significant differences in the percentage of detachment and breakage of the retainers between the groups. Results: The maxillary detachment rates were 21.42% for the GFR group and 22.22% for the MST group; the mandibular detachment rates were 11.76% for the GFR group and 15.62% for the MST group. The maxillary breakage rates were 7.14% for the GFR group and 16.66% for the MST group; the mandibular breakage rates were 8.82% for the GFR group and 15.62% for the MST group. The differences were not statistically significant. Conclusion: GFR and multistranded stainless steel retainers showed similar results in terms of bond failure and breakage after 6 years of retention. The use of GFR retainers as a retention strategy should not be discouraged and could be considered a viable esthetic alternative to stainless steel wire retainers. © 2011 CEO

Évaluation des échecs après six ans de contention : comparaison des appareils de contention en composite renforcé aux fibres (CRF) et les fils torsades collés

Objectives: The aim of this study was to compare bond failure and breakage rates of two types of bonded lingual orthodontic retainers (GFR and .0175" multistranded stainless steel wire [MST]) after a 6-year retention period. Subjects and methods: Eighty-five young adults were randomly divided into two groups: 40 subjects received 48 GFR retainers (14 maxillary and 34 mandibular retainers), and 45 subjects received 50 MST retainers (18 maxillary and 32 mandibular retainers). A Fisher's exact test was performed in order to identify significant differences in the percentage of detachment and breakage of the retainers between the groups. Results: The maxillary detachment rates were 21.42% for the GFR group and 22.22% for the MST group; the mandibular detachment rates were 11.76% for the GFR group and 15.62% for the MST group. The maxillary breakage rates were 7.14% for the GFR group and 16.66% for the MST group; the mandibular breakage rates were 8.82% for the GFR group and15.62% for the MST group. The differences were not statistically significant. Conclusion: GFR and multistranded stainless steel retainers showed similar results in terms of bond failure and breakage after 6 years of retention. The use of GFR retainers as a retention strategy should not be discouraged and could be considered a viable esthetic alternative to stainless steel wire retainers. © 2011 CEO