Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip

UV stimulation of prostate cells causes an apoptotic response that is dependent on the zinc finger transcription factor Egr1; downstream targets of Egr1 in this response were identified

A systematic literature review on antecedents of workarounds related to information systems in hospitals

The use of digital technology in the healthcare sector, and in hospitals in particular, has an impact on daily routine and on the quality of patient care. In dynamic organizations like hospitals, where urgent needs have to be met, employees develop workarounds for different reasons, which in turn can have a negative impact on the quality of patient care. While the existing literature focusses mainly on the consequences of IT mismatch to work practices in hospitals and classifications thereof, a holistic understanding of the underlying reasons for workers’ deviating behavior is missing. This article begins to close this research gap with a systematic literature review on antecedents of workarounds. The analysis provides a valuable contribution for both research and practice, as measuring root causes for behavior enables organizations to understand and develop control mechanisms

TOWARDS A TAXONOMY OF DIGITAL TWIN APPLICATIONS FOR TELEMEDICAL HEALTHCARE

The industrial paradigm of a Digital Twin (DT), a virtual representation of a physical object, promises an impactful opportunity to advance digital healthcare. Especially in telemedicine, the application of DTs could yield various benefits for patients, providers, and payers. However, the development of DTs for healthcare and specifically telemedicine is not yet mature. Therefore, this research in progress paper attempts to structure the research field and classify DTs for digital health and in future, for telemedicine. Based on a systematic literature review (SLR) and grounded theory analysis techniques, we derive 12 dimensions and 35 characteristics that support researchers and practitioners to develop, apply, refine and evaluate various DT applications. The taxonomy serves as a promising starting point for further research on implementing or adopting DTs in healthcare and telemedicine. An application of a real-world objective already shows the feasibility of our taxonomy

DIGITISATION ALONG THE PATIENT PATHWAY IN HOSPITALS

The European health care system faces massive challenges due to different influences such as an aging population, multi-morbidity and an innovation-sceptical industry. Above all, hospitals are in the focus of interest, given their central importance and networking of the supply system. Due to a lower availability of resources, but at the same time increasing demands and expectations on the quality of care, hospitals are under particular pressure to optimise. Digital technologies and information systems promise valuable potential along the patient pathway with regard to increasing efficiency and improving the quality of care. This paper provides a structured literature review on the digitisation of the patient\u27s pathway and closes the research gap to a holistic view. As part of a quantitative empirical research study, 130 German hospitals were surveyed regarding their digital potential. Therefore, a cross-sectional study was conducted to assess the current state of digital documentation and communication, as well as the identification of digitisation and integration in hospitals. The study shows interesting results regarding digital potentials, particularly concerning the internal and external communication of hospitals

General Practitioners’ Attitudes Toward Artificial Intelligence–Enabled Systems: Interview Study

Background: General practitioners (GPs) care for a large number of patients with various diseases in very short timeframes under high uncertainty. Thus, systems enabled by artificial intelligence (AI) are promising and time-saving solutions that may increase the quality of care. Objective: This study aims to understand GPs’ attitudes toward AI-enabled systems in medical diagnosis. Methods: We interviewed 18 GPs from Germany between March 2020 and May 2020 to identify determinants of GPs’ attitudes toward AI-based systems in diagnosis. By analyzing the interview transcripts, we identified 307 open codes, which we then further structured to derive relevant attitude determinants. Results: We merged the open codes into 21 concepts and finally into five categories: concerns, expectations, environmental influences, individual characteristics, and minimum requirements of AI-enabled systems. Concerns included all doubts and fears of the participants regarding AI-enabled systems. Expectations reflected GPs’ thoughts and beliefs about expected benefits and limitations of AI-enabled systems in terms of GP care. Environmental influences included influences resulting from an evolving working environment, key stakeholders’ perspectives and opinions, the available information technology hardware and software resources, and the media environment. Individual characteristics were determinants that describe a physician as a person, including character traits, demographic characteristics, and knowledge. In addition, the interviews also revealed the minimum requirements of AI-enabled systems, which were preconditions that must be met for GPs to contemplate using AI-enabled systems. Moreover, we identified relationships among these categories, which we conflate in our proposed model. Conclusions: This study provides a thorough understanding of the perspective of future users of AI-enabled systems in primary care and lays the foundation for successful market penetration. We contribute to the research stream of analyzing and designing AI-enabled systems and the literature on attitudes toward technology and practice by fostering the understanding of GPs and their attitudes toward such systems. Our findings provide relevant information to technology developers, policymakers, and stakeholder institutions of GP care.</p

NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli.

P(i) uptake in the small intestine occurs predominantly through the NaPi-2b (SLC34a2) co-transporter. NaPi-2b is regulated by changes in dietary P(i) but the mechanisms underlying this regulation are largely undetermined. Sequence analyses show NaPi-2b has a PDZ binding motif at its C terminus. Immunofluorescence imaging shows NaPi-2b and two PDZ domain containing proteins, NHERF1 and PDZK1, are expressed in the apical microvillar domain of rat small intestine enterocytes. Co-immunoprecipitation studies in rat enterocytes show that NHERF1 associates with NaPi-2b but not PDZK1. In HEK co-expression studies, GFP-NaPi-2b co-precipitates with FLAG-NHERF1. This interaction is markedly diminished when the C-terminal four amino acids are truncated from NaPi-2b. FLIM-FRET analyses using tagged proteins in CACO-2(BBE) cells show a distinct phasor shift between NaPi-2b and NHERF1 but not between NaPi-2b and the PDZK1 pair. This shift demonstrates that NaPi-2b and NHERF1 reside within 10 nm of each other. NHERF1(-/-) mice, but not PDZK1(-/-) mice, had a diminished adaptation of NaPi-2b expression in response to a low P(i) diet. Together these studies demonstrate that NHERF1 associates with NaPi-2b in enterocytes and regulates NaPi-2b adaptation