Effect of blue light on the photosynthesis and flavonoid accumulation in leaves of Hedyotis corymbosa (L.) Lam.

In plants, blue light with a short wavelength can promote light reaction in photosynthesis and increase dry mass. Photosynthesis plays an important role in supporting carbohydrates for primary and secondary metabolism processes. Flavonoids are phenolic compounds, a class of plant secondary metabolites, that can be obtained from many medicinal herbs. These phenolic compounds are involved in the reactive oxygen species scavenging system, inhibit lipid peroxidation by free-radical, chelate redox-active metals resulting in their antioxidant ability and cardioprotective effects. In this study, H. corymbosa (L.) Lam., one of the common medicinal herbs, was cultured for 4 weeks under conditions of 450 nm blue LED (light-emitting diode) lights at the different light intensity as treatments and fluorescent lamp light as a control to investigate the effects of blue light on photosynthesis and flavonoid accumulation in leaves. The results show that blue light at 450 nm promoted photosynthetic rate by enhancing stomatal opening, electron transport rate in light reaction. Blue light also enhanced photoprotection by decrease the quantum yield of non-photochemical losses, increase the quantum yield of non-photochemical quenching and gained 24% more in dry mass. The accumulation of flavonoid and total phenolic compounds in leaves was followed by a decrease in sucrose. These events proved that blue light enhances photosynthesis and increase carbohydrate and flavonoid accumulation in leaves

Potential Use of Colored LED Lights to Increase the Production of Bioactive Metabolites Hedyotis corymbosa (L.) Lam

Hedyotis corymbosa (L.) Lam is a wild herb that is used in traditional Indian, Chinese, and African medicine. Light-emitting diode (LED) technology is paving the way to enhance crop production and inducing targeted photomorphogenic, biochemical, or physiological responses in plants. This study examines the efficiency of H. corymbosa (L.) Lam production under blue 450 nm and red 660 nm LED lights for overall plant growth, photosynthetic characteristics, and the contents of metabolite compounds. Our research showed that blue LED lights provided a positive effect on enhancing plant growth and overall biomass. In addition, blue LED lights are more effective in controlling the production of sucrose, starch, total phenolic compounds, and total flavonoid compared to red LED lights. However, blue and red LED lights played essential but different roles in photosynthetic characteristics. Our results showed the potential of colored LED light applications in improving farming methods and increasing metabolite production in herbs

Colored LED Lights: Use One Color Alone or with Others for Growth in <i>Hedyotis corymbosa</i> In Vitro?

In recent years, light-emitting diode (LED) technology has been applied to improve crop production and induce targeted biochemical or physiological responses in plants. This study investigated the effect of different ratios of blue 450 nm and red 660 nm LEDs on the overall plant growth, photosynthetic characteristics, and total triterpenoid production in the leaves of Hedyotis corymbosa in vitro plants. The results showed that a high proportion of blue LED lights had a positive effect on enhancing photosynthesis and the overall biomass. In addition, blue LED lights were shown to be more effective in controlling the production of the total triterpenoid content compared with the red LED lights. Moreover, it was also found that plants grown under a high proportion of red LEDs exhibited reduced photosynthetic properties and even induced damage to the photosynthetic apparatus, which indicated that the blue or red LED lights played contrary roles in Hedyotis corymbosa

Actionable Mutation Profiles of Non-Small Cell Lung Cancer patients from Vietnamese population

Comprehensive profiling of actionable mutations in non-small cell lung cancer (NSCLC) is vital to guide targeted therapy, thereby improving the survival rate of patients. Despite the high incidence and mortality rate of NSCLC in Vietnam, the actionable mutation profiles of Vietnamese patients have not been thoroughly examined. Here, we employed massively parallel sequencing to identify alterations in major driver genes (EGFR, KRAS, NRAS, BRAF, ALK and ROS1) in 350 Vietnamese NSCLC patients. We showed that the Vietnamese NSCLC patients exhibited mutations most frequently in EGFR (35.4%) and KRAS (22.6%), followed by ALK (6.6%), ROS1 (3.1%), BRAF (2.3%) and NRAS (0.6%). Interestingly, the cohort of Vietnamese patients with advanced adenocarcinoma had higher prevalence of EGFR mutations than the Caucasian MSK-IMPACT cohort. Compared to the East Asian cohort, it had lower EGFR but higher KRAS mutation prevalence. We found that KRAS mutations were more commonly detected in male patients while EGFR mutations was more frequently found in female. Moreover, younger patients