3 research outputs found
Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae) associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) from Colombia
A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein described using morphological and molecular data. It was found parasitising the alimentary tract of the insect host Cyrtomenus bergi, a polyphagous pest. The morphology of B. cyrtomeni was investigated using light and transmission microscopy and molecular phylogeny was inferred from the sequences of spliced leader RNA (SL rRNA) - 5S rRNA gene repeats and the 18S small subunit (SSU) rRNA gene. Epimastigotes of variable size with straphanger cysts adhering to the middle of the flagellum were observed in the intestinal tract, hemolymph and Malpighian tubules. Kinetoplasts were always observed anterior to the nucleus. The ultrastructure of longitudinal sections of epimastigotes showed the flagellum arising laterally from a relatively shallow flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats were positive in all cases, producing a 0.8-kb band. The amplicons were 797-803 bp long with > 98.5% identity, indicating that they originated from the same organism. According to the sequence analysis of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni is different from all other known species or isolates of Trypanosomatidae. Both analyses indicate that among known species, it is most closely related to Blastocrithidia triatomae
Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae) associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) from Colombia
A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein
described using morphological and molecular data. It was found
parasitising the alimentary tract of the insect host Cyrtomenus bergi,
a polyphagous pest. The morphology of B. cyrtomeni was investigated
using light and transmission microscopy and molecular phylogeny was
inferred from the sequences of spliced leader RNA (SL rRNA) - 5S rRNA
gene repeats and the 18S small subunit (SSU) rRNA gene. Epimastigotes
of variable size with straphanger cysts adhering to the middle of the
flagellum were observed in the intestinal tract, hemolymph and
Malpighian tubules. Kinetoplasts were always observed anterior to the
nucleus. The ultrastructure of longitudinal sections of epimastigotes
showed the flagellum arising laterally from a relatively shallow
flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats
were positive in all cases, producing a 0.8-kb band. The amplicons were
797-803 bp long with > 98.5% identity, indicating that they
originated from the same organism. According to the sequence analysis
of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni
is different from all other known species or isolates of
Trypanosomatidae. Both analyses indicate that among known species, it
is most closely related to Blastocrithidia triatomae