21 research outputs found
Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans
No full textInternational audienceA gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 613bp were identified as fixL and fixJ. Kanamycin cartridges were inserted into the cloned fixL and fixJ genes and recombined into the host genome. The resulting mutants were Nif- Fix-, suggesting that the two genes were required for symbiotic nitrogen fixation and for nitrogen fixation in the free-living state. Using pnifH-lacZ and pnifA-lacZ fusions, it was shown that the FixLJ products controlled the expression of nifH and nifA in bacteria grown in the free-living state
Transcription of a Rhizobium ieguminosarum biovar phaseoli gene needed for melanin synthesis is activated by nifA of Rhizobium and Klebsiella pneumoniae
No full textBroad Host-Range Vector for Efficient Expression of Foreign Genes in Gram-Negative Bacteria
No full textcDNA-AFLP analysis unravels a genome-wide hrpG-regulon in the plant pathogen Xanthomonas campestris pv. vesicatoria
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An Agrobacterium catalase is a virulence factor involved in tumorigenesis
No full text10.1046/j.1365-2958.2000.01709.xMolecular Microbiology352407-414MOMI
Development of Breeding Materials of Transgenic Tomato Plants with a Truncated Replicase Gene of Cucumber Mosaic Virus for Resistance to the Virus
No full textCharacterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium Alcanivorax borkumensis
No full textPhenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strains in vitro and in vivo
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