Origin and no. of samples analysed by the modified <i>cox</i> III qPCR assay.

*<p>SA – South Africa.</p>**<p>Cattle.</p

Alignment of the probe area of the <i>cox</i> III gene sequences.

<p>The modified anchor (light blue shading) and sensor (yellow shading) probe sequences are indicated. <i>cox</i> III sequences were obtained from clones from control samples (blue text) and clones from selected African buffalo samples. Differences are based on the <i>cox</i> III sequence of <i>T. mutans</i> (ZAM/C9142.2). The identified <i>cox</i> III sequence variants are indicated.</p

Occurrence of <i>Theileria</i> species infections in buffalo and cattle samples from South Africa and Mozambique.

<p>(a) As determined by the RLB and <i>cox</i> III qPCR assays (n = 224). The number of samples with non-species specific melting temperatures on the <i>cox</i> III qPCR assay (*) and those that hybridized only with the <i>Theileria</i>/<i>Babesia</i> genus-specific probes using the RLB assay (**) are shown. (b) Comparison of the RLB, 18S qPCR and <i>cox</i> III qPCR assays for detection of <i>T. parva</i> (n = 206).</p

Results of the RLB and modified <i>cox</i> III qPCR assays for selected samples and clones.

*<p>Indicates a non-species specific melting peak located between the peaks for the species indicated in brackets.</p>**<p>Did not cluster with any of the other sequences and its identity could not be established.</p

Phylogenetic relationships of the <i>cox</i> III gene sequence variants of <i>Theileria</i> spp.

<p>The figure shows sequences identified in this study (black) with <i>Theileria</i> control sequences (bold) and published <i>Theileria</i> spp. (italics). Bootstrap values indicate the degree of support for each cluster. The tree was outgroup rooted using the <i>cox</i> III gene sequence of <i>T. annulata</i>.</p

Laboratory material used for the processing of the samples.

<p>Laboratory material used for the processing of the samples.</p

Dried blood spots (left) and buffy coats (right) on labelled filter papers.

<p>Dried blood spots (left) and buffy coats (right) on labelled filter papers.</p

Output of the <i>DPn</i>II-PCR-RFLP with lanes 1, 6, and 10 as size markers (100-bp ladder), lanes 2, 3, 4, 5, and 7 as sensitive profiles (one band), lane 8 as resistant profile (two bands), and lane 9 as mixed profile (three bands).

<p>Output of the <i>DPn</i>II-PCR-RFLP with lanes 1, 6, and 10 as size markers (100-bp ladder), lanes 2, 3, 4, 5, and 7 as sensitive profiles (one band), lane 8 as resistant profile (two bands), and lane 9 as mixed profile (three bands).</p