Geographic location of Antioquia state and the municipalities/villages included in the study.

<p>Geographic location of Antioquia state and the municipalities/villages included in the study.</p

Detection of HEV genome on wastewater samples collected on first sampling.

<p>Representative results of the positive samples amplified by RT-nested PCR targeting the ORF2/3 region (137bp). MW: Molecular Weight. 1. Girardota village 2. San Pedro de Los Milagros 3. Venecia 4.Granada 5.Arboletes 6. Cisneros 7. Nutibara village 8. Frontino 9. Puerto Berrío 10. Zaragoza. (-) negative control (+) positive control. 2% agarose gel electrophoresis stained with ethidium bromide.</p

Water treatment systems of the municipalities of Antioquia State included in the study

<p>Water treatment systems of the municipalities of Antioquia State included in the study</p

Sampling and viral concentration methodologies.

<p>Sampling and viral concentration methodologies.</p

HEV phylogenic tree based on a fragment of 99 pb ORF2/3 HEV region.

<p>Based on the Bayesian Information Criterion (BIC), the model chosen for the phylogenetic analysis was Tamura 3-parameter + gamma distribution. The tree was constructed using the Neighbor-Joining method with 1000 bootstrap replicates. Samples obtained in this study are shown with a black circle (●). Clades for genotypes 1, 2, 3 and 4 are indicated with brackets. The GenBank accession number, genotype and country of isolation identify viral strains. Ven_Res_1erMx, SanPedro_Res_1erMx and Cisneros_Res_1erMx corresponded to wastewater sample collected on the first sampling at Venecia, San Pedro de los Milagros and Cisneros municipalities, respectively.</p