NR2A, NR2B, GLT1 and GLAST protein levels striatum of 60-day-old wild type (WT) and <i>Gcdh</i>^-/- mice.

<p>β-actin was used as the endogenous control. Results are expressed as mean ± standard deviation for four independent experiments (animals) per group. *p≤0.05 compared to WT (Student's t test for unpaired samples).</p

Number of assays used for relative quantification of each glutamate receptor subunits and transporters (Applied Biosystems).

<p>Number of assays used for relative quantification of each glutamate receptor subunits and transporters (Applied Biosystems).</p

Glutamate transporter mRNA expression levels in cerebral cortex and striatum from wild type (WT) and <i>Gcdh^-/-</i> mice at 7 (A), 30 (B) and 60 (C) days of life.

<p>Results are expressed as mean ± standard deviation for five independent experiments (animals) per group. **p≤0.01, ***p≤0.001 compared to WT (Student's t test for unpaired samples).</p

Glutamate receptor mRNA expression levels in cerebral cortex and striatum from wild type (WT) and <i>Gcdh^-/-</i> mice at 7 (A), 30 (B) and 60 (C) days of life.

<p>Results are expressed as mean ± standard deviation for five independent experiments (animals) per group. *p≤0.05, **p≤0.01, ***p≤0.001 compared to WT (Student's t test for unpaired samples).</p

NMDA and non-NMDA receptor mRNA expression in cerebral cortex of 30-day-old wild type (WT) and <i>Gcdh^-/-</i> mice submitted to a normal (0.9% lysine) or high lysine (4.7%) diet.

<p>NR1 (A), NR2A (B), NR2B (C), GluR2 (D) and GluR6 (E). Results are expressed as mean ± standard deviation for five independent experiments (animals) per group. *p≤0.05 compared to WT; **p≤0.01, ***p≤0.001 compared to WT; ^#p≤0.01 compared to <i>Gcdh^-/-</i> mice submitted to a normal diet (Student's t test for unpaired samples).</p

Glutamate transporter (GLT1 and GLAST) mRNA expression in cerebral cortex (A) and striatum (B) of wild type (WT) and <i>Gcdh-/-</i> mice submitted to a normal (0.9% lysine) or high lysine (4.7%) diet.

<p>A). Results are expressed as mean ± standard deviation for five independent experiments (animals) per group. * p≤0.05, **p≤0.01 compared to WT; #p≤0.05 compared to <i>Gcdh</i>^-/- mice submitted to a normal diet (Student's t test for unpaired samples).</p

NR2A, NR2B, GLT1 and GLAST protein levels in cerebral cortex of 60-day-old wild type (WT) and <i>Gcdh</i>^-/- mice.

<p>β-actin was used as the endogenous control. Results are expressed as mean ± standard deviation for four independent experiments (animals) per group. *p≤0.05, **p≤0.01 compared to WT (Student's t test for unpaired samples).</p