Additional file 2: of Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases

Rice codon optimized DNA sequences of WT Cas9, eSpCas9(1.0), eSpCas9(1.1), and SpCas9-HF1. (PDF 296 kb

Additional file 2: of Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Figure S1. The sequences of the sgRNA expression vectors for rice and wheat. Figure S2. Product purity of plant ABE for rice genomic sites. Figure S3. Product purity of plant ABE for wheat genomic sites. Figure S4. The effect of spacer length of esgRNA on indel efficiency. Figure S5. Identification and analysis of the rice plantlets with targeted A to G conversions by pH-PABE-7-esgRNA. Figure S6. Identification and analysis of the wheat plantlets with targeted A to G conversions by PABE-7. Figure S7. Constructs used for base editing of TaDEP1 and TaGW2 and detection of transgene integration in the resultant T0 mutants. Table S1. Description of sgRNA target sites and sequences. Table S2. Potential off-target sites analyzed for OsACC-T1 endogenous genomic loci. Table S3. PCR primers used in this study. (DOC 6095 kb

Additional file 1: of Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Sequences Complete coding sequences of the PABE-1 to PABE-7 fusion cistrons optimized in this study. (DOCX 4108 kb