sj-docx-2-caj-10.1177_08465371241227424 – Supplemental material for Imaging Features of Invasive Fungal Rhinosinusitis: A Systematic Review

Supplemental material, sj-docx-2-caj-10.1177_08465371241227424 for Imaging Features of Invasive Fungal Rhinosinusitis: A Systematic Review by Anni Chen, James Pietris, Stephen Bacchi, WengOnn Chan, Alkis J. Psaltis, Dinesh Selva and WanYin Lim in Canadian Association of Radiologists Journal</p

sj-docx-1-caj-10.1177_08465371241227424 – Supplemental material for Imaging Features of Invasive Fungal Rhinosinusitis: A Systematic Review

Supplemental material, sj-docx-1-caj-10.1177_08465371241227424 for Imaging Features of Invasive Fungal Rhinosinusitis: A Systematic Review by Anni Chen, James Pietris, Stephen Bacchi, WengOnn Chan, Alkis J. Psaltis, Dinesh Selva and WanYin Lim in Canadian Association of Radiologists Journal</p

Additional file 1: Table S1. of Pooled genome wide association detects association upstream of FCRL3 with Graves’ disease

Top 30 single nucleotide polymorphism associated with Graves’ disease compared with controls in genome wide association study. This table denoted the top ranking SNPs and highlighted SNPs in major histocompatibility complex region and non-HLA SNPs that were chosen for subsequent validation. (DOCX 141 kb

Additional file 2: Figure S1. of Pooled genome wide association detects association upstream of FCRL3 with Graves’ disease

Scatterplot for odds ratio of 13 effect allele frequencies comparing pooled GWAS Graves’ disease with individual genotyping in the discovery cohort. This figure showed the correlation between odds ratio derived from pooled GWAS genotyping and individual genotyping in the ATOR discovery cohort; the correlation is high r = 0.89. (TIF 9765 kb

IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease

<div><p>Objective</p><p>IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases.</p><p>Methods</p><p>We organized an international consortium to collect orbital biopsies from 108 subjects including 22 with no known orbital disease, 42 with nonspecific orbital inflammatory disease (NSOI), 26 with thyroid eye disease (TED), 12 with sarcoidosis, and 6 with granulomatosis with polyangiitis (GPA). Lacrimal gland and orbital adipose tissue biopsies were immunostained for IgG4 or IgG secreting plasma cells. RNA transcripts were quantified by Affymetrix arrays.</p><p>Results</p><p>None of the healthy controls or subjects with TED had substantial IgG4 staining. Among the 63 others, the prevalence of significant IgG4-immunostaining ranged from 11 to 39% depending on the definition for significant. IgG4 staining was detectable in the majority of tissues from subjects with GPA and less commonly in tissue from subjects with sarcoidosis or NSOI. The detection of IgG4+ cells correlated with inflammation in the lacrimal gland based on histology. IgG4 staining tissue expressed an increase in transcripts associated with inflammation, especially B cell-related genes. Functional annotation analysis confirmed this.</p><p>Conclusion</p><p>IgG4+ plasma cells are common in orbital tissue from patients with sarcoidosis, GPA, or NSOI. Even using the low threshold of 10 IgG4+ cells/high powered field, IgG4 staining correlates with increased inflammation in the lacrimal gland based on histology and gene expression.</p></div

IgG4 status is independent of age and gender.

<p>*Ages are not available for 4 subjects.</p><p>IgG4 status is independent of age and gender.</p

Case demographics.

<p>*Ages are not available for 4 subjects.</p><p>Case demographics.</p

Lacrimal tissues from NSOI patients with at least 10 IgG4+ PC/hpf show increased fibrosis and inflammation.

<p>IgG4+ orbit tissues lack the lowest fibrosis and inflammation scores. Each symbol represents the score for one subject. P-values are based on Mann-Whitney U-test.</p

Examples of gene expression differences comparing IgG4+ to IgG4- orbital tissue from subjects with NSOI, GPA, or sarcoidosis.

<p>Probe sets were selected for expression increases or decreases and ample annotation. A relevant Gene Ontology Biological Process (<a href="http://geneontology.org/" target="_blank">http://geneontology.org/</a>) is listed for each gene.</p><p>Examples of gene expression differences comparing IgG4+ to IgG4- orbital tissue from subjects with NSOI, GPA, or sarcoidosis.</p

Examples of gene expression differences comparing IgG4+ to IgG4- lacrimal gland tissue from subjects with NSOI, GPA, or sarcoidosis.

<p>Probe sets were selected for expression increases of more than 2.1 fold or decreases of more than 1.5 fold, p values <0.5, and sample annotation. A relevant Gene Ontology Biological Process (<a href="http://geneontology.org/" target="_blank">http://geneontology.org/</a>) is listed for each gene.</p><p>Examples of gene expression differences comparing IgG4+ to IgG4- lacrimal gland tissue from subjects with NSOI, GPA, or sarcoidosis.</p