Additional file 1: of Molecular characterization of invasive meningococcal isolates in Burkina Faso as the relative importance of serogroups X and W increases, 2008–2012

(PDF) Unrooted phylogeny of international NmW CC11 isolates. The 128 isolates from this study are identified by black squares, the Hajj-related outbreak isolate is identified by a black star, and the remaining 470 isolates are identified according to the categories defined by from Lucidarme et al. [23]. Arrows mark isolates that are not in the same category as the most closely related isolates: the Hajj-related outbreak isolate (M07149) and an “Anglo/French Hajj strain” isolate collected in France during 2014 (M14 240,446). The tree is scaled by the number of parsimonious substitutions per branch, identified by kSNP3. Branches with bootstrap support < 70% have been deleted. (PDF 33 kb

Diagnostic sensitivity and specificity for the real-time PCR tests by latent class analysis.

<p>Diagnostic sensitivity and specificity for the real-time PCR tests by latent class analysis.</p

Cerebrospinal fluid specimens that were positive by direct real-time PCR using PerfeCTa and negative by traditional real-time PCR using TaqMan or direct real-time PCR using 5x Omni.

<p>Cerebrospinal fluid specimens that were positive by direct real-time PCR using PerfeCTa and negative by traditional real-time PCR using TaqMan or direct real-time PCR using 5x Omni.</p

Lower limits of detection for the real-time PCR tests using traditional and direct methods.^a

<p>Lower limits of detection for the real-time PCR tests using traditional and direct methods.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147765#t002fn001" target="_blank">^a</a></p

Final concentrations of primers and probes for the real-time PCR tests for detection of <i>Neisseria meningitidis</i> and its 6 serogroups, <i>Haemophilus influenzae</i>, and <i>Streptococcus pneumoniae</i> using traditional and direct methods^a.

<p>Final concentrations of primers and probes for the real-time PCR tests for detection of <i>Neisseria meningitidis</i> and its 6 serogroups, <i>Haemophilus influenzae</i>, and <i>Streptococcus pneumoniae</i> using traditional and direct methods<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147765#t001fn001" target="_blank">^a</a>.</p

Flow diagram of the national case-based meningitis surveillance system, Burkina Faso, 2011–2013.

<p>Abbreviation: rt-PCR, real-time polymerase chain reaction. *Specimens can be tested by multiple methods.</p

Cases reported through national meningitis surveillance by age group and etiology, Burkina Faso, 2011–2013.

<p>Cases reported through national meningitis surveillance by age group and etiology, Burkina Faso, 2011–2013.</p

Distribution of pneumococcal serotypes by age, Burkina Faso, 2011–2013.

<p>Distribution of pneumococcal serotypes by age, Burkina Faso, 2011–2013.</p

Epidemic curve of all suspected, probable, and confirmed meningitis cases by month, Burkina Faso, 2011–2013.

<p>Epidemic curve of all suspected, probable, and confirmed meningitis cases by month, Burkina Faso, 2011–2013.</p

Annual incidence (cases per 100,000 persons) of suspected, probable, and confirmed meningitis, Burkina Faso, 2011–2013.

<p>Annual incidence (cases per 100,000 persons) of suspected, probable, and confirmed meningitis, Burkina Faso, 2011–2013.</p