Supplementary data for the article: Dimitrijević, T.; Vulić, P.; Manojlović, D.; Nikolić, A. S.; Stanković, D. M. Amperometric Ascorbic Acid Sensor Based on Doped Ferrites Nanoparticles Modified Glassy Carbon Paste Electrode. Analytical Biochemistry 2016, 504, 20–26. https://doi.org/10.1016/j.ab.2016.03.020

Supplementary material for: [https://doi.org/10.1016/j.ab.2016.03.020]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1950

Supplementary data for the article: Dimitrijević, T.; Vulić, P.; Manojlović, D.; Nikolić, A. S.; Stanković, D. M. Amperometric Ascorbic Acid Sensor Based on Doped Ferrites Nanoparticles Modified Glassy Carbon Paste Electrode. Analytical Biochemistry 2016, 504, 20–26. https://doi.org/10.1016/j.ab.2016.03.020

Supplementary material for: [https://doi.org/10.1016/j.ab.2016.03.020]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1950

Amperometric ascorbic acid sensor based on doped ferrites nanoparticles modified glassy carbon paste electrode

In this study, a novel electrochemical sensor for quantification of ascorbic acid with amperometric detection in physiological conditions was constructed. For this purpose, cobalt and nickel ferrites were synthesized using microwave and ultrasound assistance, characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray powder diffraction (XRPD), and used for modification of glassy carbon paste electrode (GCPE). It was shown that introducing these nanoparticles to the structure of GCPE led to increasing analytical performance. Co ferrite modified GCPE (CoFeGCPE) showed better characteristics toward ascorbic acid sensing. The limit of detection (LOD) obtained by sensor was calculated to be 0.0270 mg/L, with linear range from 0.1758 to 2.6010 mg/L. This sensor was successfully applied for practical analysis, and the obtained results demonstrated that the proposed procedure could be a promising replacement for the conventional electrode materials and time-consuming and expensive separation methods. (C) 2016 Elsevier Inc. All rights reserved.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3336

Cytotoxic and cytoselective profile of novel ruthenium(II)-arene complexes with (fluoro substituated) picolinic acid

Background: Ruthenium containing compounds represent the most promising alternative to platinum-based chemotherapeutics whose therapeutic value has been limited by significant side effects. Advantageous features such as good aqueous solubility and relatively inert arene ligand make them very attractive for structural optimizations aimed for improved in vivo potency. Material and Methods: Reported complexes were obtained in a reaction of [Ru(η6-benzene)Cl(μ-Cl)]2 or [Ru(η6-toluene)Cl(μ-Cl)]2 with picolinic acid or 6-fluoropicolinic acid in a 1:2 molar ratio in ethanol and characterized by IR and NMR spectroscopy and MS spectrometry. Results: The cytotoxic profile was investigated by the colorimetric MTT assay, in a panel of human non-malignant cell line (MRC-5), and cancer cell lines (A549, HTB177, PC3, A375, HeLa, HCT116, MDA-MB-453). The complexes carrying picolinic acid, displayed moderate antiproliferative effect particularly toward colorectal carcinoma (HCT116) and cervix adenocarcinoma cells (HeLa). The highest activity and cytoselectivity was observed for complex with [Ru(η6-benzene)Cl(μ-Cl)]2 toward HCT116 cells: it was capable of reducing viability of HCT116 cells 1.5 times more efficiently (IC50 = 27.5 μM), than of the MRC-5 cells (IC50 = 41.3 μM). Conclusion: The complex with improved activity and selectivity is candidate for further investigations regarding its binding modes, sites, and affinities