Improvement of the 3ω\omega thermal conductivity measurement technique at nanoscale

The reduction of the thermal conductivity in nanostructures opens up the possibility of exploiting for thermoelectric purposes also materials such as silicon, which are cheap, available and sustainable but with a high thermal conductivity in their bulk form. The development of thermoelectric devices based on these innovative materials requires reliable techniques for the measurement of thermal conductivity on a nanometric scale. The approximations introduced by conventional techniques for thermal conductivity measurements can lead to unreliable results when applied to nanostructures, because heaters and temperature sensors needed for the measurement cannot have a negligible size, and therefore perturb the result. In this paper we focus on the 3$\omega$ technique, applied to the thermal conductivity measurement of suspended silicon nanomembranes. To overcome the approximations introduced by conventional analytical models used for the interpretation of the 3$\omega$ data, we propose to use a numerical solution, performed by means of finite element modeling, of the thermal and electrical transport equations. An excellent fit of the experimental data will be presented, discussed, and compared with an analytical model

DATA ON ARTS ORGANIZATIONS: A REVIEW AND NEEDS ASSESSMENT, WITH DESIGN IMPLICATIONS

This project describes the data resources on arts organizations that are currently available to inform the efforts of policy makers, arts managers, and researchers working in the arts field. It assesses the adequacy of different data sources for identifying the population of arts and cultural organizations in a community. The report is based on a review of more than a dozen sources of information about arts and cultural organizations, interviews with researchers and data specialists, and an empirical study of arts organizations in three metropolitan areas - Philadelphia, Dallas-Fort Worth, and Minneapolis-St. Paul. The report concludes with recommendations for improving data quality and for establishing an ongoing national database on the arts sector.

Effects of Salinity on Reproduction and Survival of the Calanoid Copepod Pseudodiaptomus Pelagicus

Four experiments were conducted on the calanoid copepod, Pseudodiaptomus pelagicus, to determine the effects of salinity on survival, development time, reproductive output, and population growth in order to define the optimal salinity for culture. To determine the appropriate experimental salinity range we exposed nauplii and adults to abrupt salinity changes from 35 g/L to 5, 10, 15, 35, 42, and 48 g/L at 30 °C and determined survival after 24 hours. The second experiment stocked early stage nauplii into 1 L beakers after which they were cultured using standard procedures for 10 days at six salinities (10, 15, 20, 25, 30, 35 g/L); from this survival, sex ratio, time to maturation, and fecundity were measured. The third experiment evaluated the effects of salinity on brood size, brood interval, and nauplii production by stocking individual adult pairs and monitoring nauplii production daily for 10 days. The fourth experiment determined the effects of salinity on population growth and composition of the population produced by stocking 10 adult pairs and culturing them until five days after the first mature adults were observed. Results from the abrupt salinity change experiment showed nauplii survival decreased following abrupt changes in salinity from 35 g/L to \u3c 15 g/L and \u3e 35 g/L. Additionally, adults do not tolerate rapid changes in salinity from 35 g/L to \u3c 15 g/L but are rather tolerant of changes in salinity up to 48 g/L. Survival from early nauplii to adult was not significantly affected by salinity but survival declined at 35 g/L. Time to first maturation and maturation of the entire population was significantly influenced by salinity and took from 6.3 to 9.5 days. In the individual paired adults experiment, salinity significantly affected nauplii production by affecting brood interval and brood size. The percentage of ovigerous females peaked at 20 g/L and declined at salinities above and below this value. When developing production objectives, aquaculturists must consider salinity because of its numerous effects on the culture of P. pelagicus. The optimal salinity range to achieve high survival and the greatest nauplii production is 15–25 g/L

Skilling seniors in computers: Community training responses to the digital divide

As computers pervade further the lives of older adults the need for relevant affordable training grows. Older adults, not through frailty or age but because accessing training can be difficult, do experience the digital divide sharply. The intent of this paper is to suggest successful ways of teaching computers through the discussion of a case study of Skylarkers 60 and Better Program and a longitudinal study conducted there. Centre managers can benefit from knowing the types of teaching methods that may make their computer training beneficial and useful to older adults

Cause and context: place-based approaches to investigate how environments affect mental health

Objectives Our surroundings affect our mood, our recovery from stress, our behavior, and, ultimately, our mental health. Understanding how our surroundings influence mental health is central to creating healthy cities. However, the traditional observational methods now dominant in the psychiatric epidemiology literature are not sufficient to advance such an understanding. In this essay we consider potential alternative strategies, such as randomizing people to places, randomizing places to change, or harnessing natural experiments that mimic randomized experiments. Methods We discuss the strengths and weaknesses of these methodological approaches with respect to (1) defining the most relevant scale and characteristics of context, (2) disentangling the effects of context from the effects of individuals’ preferences and prior health, and (3) generalizing causal effects beyond the study setting. Results Promising alternative strategies include creating many small-scale randomized place-based trials, using the deployment of place-based changes over time as natural experiments, and using fluctuations in the changes in our surroundings in combination with emerging data collection technologies to better understand how surroundings influence mood, behavior, and mental health. Conclusions Improving existing research strategies will require interdisciplinary partnerships between those specialized in mental health, those advancing new methods for place effects on health, and those who seek to optimize the design of local environments

Global turnover of histone post-translational modifications and variants in human cells

<p>Abstract</p> <p>Background</p> <p>Post-translational modifications (PTMs) on the N-terminal tails of histones and histone variants regulate distinct transcriptional states and nuclear events. Whereas the functional effects of specific PTMs are the current subject of intense investigation, most studies characterize histone PTMs/variants in a non-temporal fashion and very few studies have reported kinetic information about these histone forms. Previous studies have used radiolabeling, fluorescence microscopy and chromatin immunoprecipitation to determine rates of histone turnover, and have found interesting correlations between increased turnover and increased gene expression. Therefore, histone turnover is an understudied yet potentially important parameter that may contribute to epigenetic regulation. Understanding turnover in the context of histone modifications and sequence variants could provide valuable additional insight into the function of histone replacement.</p> <p>Results</p> <p>In this study, we measured the metabolic rate of labeled isotope incorporation into the histone proteins of HeLa cells by combining stable isotope labeling of amino acids in cell culture (SILAC) pulse experiments with quantitative mass spectrometry-based proteomics. In general, we found that most core histones have similar turnover rates, with the exception of the H2A variants, which exhibit a wider range of rates, potentially consistent with their epigenetic function. In addition, acetylated histones have a significantly faster turnover compared with general histone protein and methylated histones, although these rates vary considerably, depending on the site and overall degree of methylation. Histones containing transcriptionally active marks have been consistently found to have faster turnover rates than histones containing silent marks. Interestingly, the presence of both active and silent marks on the same peptide resulted in a slower turnover rate than either mark alone on that same peptide. Lastly, we observed little difference in the turnover between nearly all modified forms of the H3.1, H3.2 and H3.3 variants, with the notable exception that H3.2K36me2 has a faster turnover than this mark on the other H3 variants.</p> <p>Conclusions</p> <p>Quantitative proteomics provides complementary insight to previous work aimed at quantitatively measuring histone turnover, and our results suggest that turnover rates are dependent upon site-specific post-translational modifications and sequence variants.</p

Empathic and cognitive processing in people with schizophrenia: a study on an Italian sample

The aim of this study was to explore the relationships among empathy processes in terms of self-report empathy evaluation and recognition of emotional cues and Theory of Mind components. We used the Empathy Quotient – short form (EQ-s), the Pictures of Facial Affect (POFA) system, a (ToM) Irony appreciation task and the Wisconsin Card Sorting Test (WCST), respectively. The Positive and Negative Symptoms Scale (PANSS) and Global Assessment of Functioning (GAF) were also used to investigate the relationship with symptomatology and functioning. The sample consisted of 30 participants with diagnosis of schizophrenia. Our results found no significant correlations between EQ-s and other cognitive or clinical variables. PoFA total score and recognition of fear correlated with time spent to give a correct response to the ToM irony comprehension. Time spent to correctly respond to both ToM and physical vignettes correlated with negative symptoms. Positive, negative and cognitive clusters of the PANSS correlated with the GAF. The relationships we found among the considered constructs suggest that empathic processing acts on functionality improving the personal efficiency, in terms of readiness and rapidity, to cope with one’s environment, in the multifaceted social setting. Given that emotion perception in particular has been connected to social competence, independent living and community functioning, it is conceivable that emotion processing may be a potential catalyst within the mindreading process, which can have an impact both on symptomatology and social functioning

An Agent-Based Model of Collective Emotions in Online Communities

We develop a agent-based framework to model the emergence of collective emotions, which is applied to online communities. Agents individual emotions are described by their valence and arousal. Using the concept of Brownian agents, these variables change according to a stochastic dynamics, which also considers the feedback from online communication. Agents generate emotional information, which is stored and distributed in a field modeling the online medium. This field affects the emotional states of agents in a non-linear manner. We derive conditions for the emergence of collective emotions, observable in a bimodal valence distribution. Dependent on a saturated or a superlinear feedback between the information field and the agent's arousal, we further identify scenarios where collective emotions only appear once or in a repeated manner. The analytical results are illustrated by agent-based computer simulations. Our framework provides testable hypotheses about the emergence of collective emotions, which can be verified by data from online communities.Comment: European Physical Journal B (in press), version 2 with extended introduction, clarification

The liminality of trajectory shifts in institutional entrepreneurship

In this paper, we develop a process model of trajectory shifts in institutional entrepreneurship. We focus on the liminal periods experienced by institutional entrepreneurs when they, unlike the rest of the organization, recognize limits in the present and seek to shift a familiar past into an unfamiliar and uncertain future. Such periods involve a situation where the new possible future, not yet fully formed, exists side-by-side with established innovation trajectories. Trajectory shifts are moments of truth for institutional entrepreneurs, but little is known about the underlying mechanisms of how entrepreneurs reflectively deal with liminality to conceive and bring forth new innovation trajectories. Our in-depth case study research at CarCorp traces three such mechanisms (reflective dissension, imaginative projection, and eliminatory exploration) and builds the basis for understanding the liminality of trajectory shifts. The paper offers theoretical implications for the institutional entrepreneurship literature

Isolation and Characterization of Precise Dye/Dendrimer Ratios

Fluorescent dyes are commonly conjugated to nanomaterials for imaging applications using stochastic synthesis conditions that result in a Poisson distribution of dye/particle ratios and therefore a broad range of photophysical and biodistribution properties. We report the isolation and characterization of generation 5 poly(amidoamine) (G5 PAMAM) dendrimer samples containing 1, 2, 3, and 4 fluorescein (FC) or 6‐carboxytetramethylrhodamine succinimidyl ester (TAMRA) dyes per polymer particle. For the fluorescein case, this was achieved by stochastically functionalizing dendrimer with a cyclooctyne “click” ligand, separation into sample containing precisely defined “click” ligand/particle ratios using reverse‐phase high performance liquid chromatography (RP‐HPLC), followed by reaction with excess azide‐functionalized fluorescein dye. For the TAMRA samples, stochastically functionalized dendrimer was directly separated into precise dye/particle ratios using RP‐HPLC. These materials were characterized using 1 H and 19 F NMR spectroscopy, RP‐HPLC, UV/Vis and fluorescence spectroscopy, lifetime measurements, and MALDI. High definition : Two approaches for the formation of generation 5 PAMAM samples containing precise dye/dendrimer ratios are presented. The first approach, using direct separation based on dye hydrophobicity, generated a set of TAMRA‐containing dendrimers, and the second, using click chemistry, generated a set of fluorescein‐containing dendrimer (see figure).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106970/1/chem_201304854_sm_miscellaneous_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/106970/2/4638_ftp.pd