Determining whether mate-searching males use directional information from female signals in Umbonia crassicornis

Abstract only availableVibrational communication is widespread in insect social and ecological interactions. In fact, it has been estimated that thousands of insect species use vibrational signals as a primary source of communication. During many interactions, such as courtship and predator encounters, it is necessary for individuals to locate the source of the vibrational signal. Although localization of this sort is important, the mechanisms that small insects use to locate the source of the vibrational signal are not known. We are studying mate localization in thornbug treehoppers, Umbonia crassicornis (Hemiptera: Membracidae). These are small sap-feeding insects in which mate-searching males produce vibrational signals that are transmitted along the stems and leaves of their host plant during courtship. First, the male produces a vibrational signal that is detected by the female. The female responds with her own vibration, creating a duet which is repeated until the male reaches the female. We hypothesize that the female's vibrational signals provide directional information to searching males. In order to test this hypothesis, we recorded the routes traveled and decisions made by mate-searching males as well as audio of the male-female duets. Our preliminary data suggest that female signals do influence the paths that males take to find females. Our results will guide future research on the actual mechanisms used by treehoppers to determine the direction of a substrate vibration and will continue to provide important insights into insect social behavior, ecology, and evolution.NSF Undergraduate Mentoring in Environmental Biolog

Dataset on Galanin Receptor 3 mutants that improve recombinant receptor expression and stability in an agonist and antagonist bound form

Galanin Receptor 3 (GALR3) is a G-protein-coupled receptor with a widespread distribution in the brain and plays a role in a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. Therefore, GALR3 is considered an attractive CNS drug target (Freimann et al., 2015) [1]. This dataset contains GALR3 point mutants that improve recombinant protein expression and thermal stability of the receptor contained in virus-like particles (VLPs) or obtained by detergent-purification of baculovirus-infected insect cells. The mutations listed can be grouped in those that improve the stability of the agonist-bound and the antagonist-bound form of the receptor. Protein characteristics in terms of protein expression and thermal stability were comparable between GPCR-VLP and GPCR overexpressing Sf9 cultures. The further analysis and detailed results of these mutants as well as their impact on biophysical assay development for drug discovery can be found in “Method for Rapid Optimization of Recombinant GPCR Protein Expression and Stability using Virus-Like Particles” (Ho et al., 2017) [2]