International Journal of Current Microbiology and Applied Sciences

Not AvailableNineteen F5 individual populations selected from two crosses involving two Gossypium hirsutum cotton varieties RS875 and F1861 with South American germplasm lines SA524 and SA668 were evaluated in randomized block design with three replications in the Kharif season of 2016-17. The objective of study was to develop high yielding (>20quintals/hectare) and high ginning out turn per cent (>35) cultures/ varieties. Out of 19 advance generation, three advance generation individual populations RS875xSA524 P12, F1861xSA668 P15, RS875xSA524 P4 had significantly higher seed cotton yield in comparison to both the check varieties H1226 (1717.37?292.3) and (F1861 1751.09 ?292.3) and possessed GOT% of 40.6, 38.5 and 39.0 and lint yield 935.6, 848.0 and772.4kg/ hectare, respectively. The present study reveals good scope for isolation of pure lines from the advance generation progenies of heterotic F1s for high seed cotton yield and high ginning out turn

Diversity and recombination analysis of Cotton leaf curl Multan virus: a highly emerging begomovirus in northern India

Abstract Background Cotton leaf curl disease (CLCuD), caused by begomoviruses in association with satellite molecules, is a major threat to cotton production causing enormous losses to cotton crop in most of the cotton growing countries including Indian subcontinent. In this study, isolates of begomovirus and satellite molecules associated with CLCuD were collected from North India (Haryana, New Delhi). They were amplified employing rolling circle replication mechanism, cloned, sequenced and, their phylogenetic and recombination analysis was performed. Results The five Cotton leaf curl Multan virus (CLCuMuV) isolates investigated in this study showed monopartite organization of the genome typical of Old World begomoviruses. Nucleotide sequence analyses assigned them as the strains of CLCuMuV and were designated as CLCuMuV-SR13, CLCuMuV-SR14, CLCuMuV-ND14, CLCuMuV-ND15 and CLCuMuV-SR15. The genome of CLCuMuV-SR13 shared a highest level of nucleotide sequence identity (98%) with CLCuMuV (JN678804), CLCuMuV-SR14 and CLCuMuV-SR15 exhibited 96% with CLCuMuV (KM096471), while isolates CLCuMuV-ND15 and CLCuMuV-SR15 revealed 96% sequence identity with CLCuMuV (AY765253). The four betasatellite molecules investigated in this study shared 95–99% nucleotide sequence identity with Cotton leaf curl Multan betasatellite (CLCuMB) from India. The betasatellite molecules were designated as CLCuMB-SR13, CLCuMB-SR14, CLCuMB-ND14 and CLCuMB-ND15. Alphasatellite molecules in this study, designated as GLCuA-SR14, GLCuA-ND14 and GLCuA-SR15, revealed 98% identity with Guar leaf curl alphasatellite (GLCuA) reported from Pakistan. Conclusion The phylogenetic and recombination studies concluded that the isolates of CLCuMuV genomes undertaken in this study have a potential recombinant origin. Remarkably, significant recombination was detected in almost all the genes with contribution of Cotton leaf curl Kokhran Virus (CLCuKoV) in IR, V1, V2, C1, C4 and C5 regions and of CLCuMuV in C2 region of CLCuMuV-SR14. CLCuKoV also donated in C2, C3 regions of CLCuMuV-ND14; V1, V2, C2 and C3 regions of CLCuMuV-ND15 and C1 of CLCuMuV-SR15. Altogether, these observations signify the uniqueness in Indian CLCuMuV isolates showing contribution of CLCuKoV in all the genes. An interesting observation was frequent identification of GLCuA in CLCuD leaf samples

Collimation of the output beam from an asymmetric unstable ring resonator

A simple method to overcome the problem of large beam divergence and compensate for the astigmatic difference in the output beam of an asymmetric unstable ring resonator is proposed. The method is based on suitably shaping the outer surface of the output mirror. The results indicate that it is possible to select the curvatures of the output surface so that a collimated output beam is obtained in the two orthogonal planes over a fairly wide range of spacings between the focusing elements

Analysis of an unstable confocal ring laser with a thermally induced active medium

Analysis of a confocal ring laser including thermal lensing effects in the active medium is presented. The relations for the stability criterion, beam magnification, and the positional shift in the beam minimum are derived in terms of the parameters of the resonator. It is suggested that by appropriately adjusting the separation between the confocal lens pair, the effects due to thermal lensing can be compensated

Design of multiwavelength thin-film polarizers for high-power laser systems

Design of a multiwavelength thin-film polarizer for high-power laser applications is presented. The desired polarizational properties are achieved by suitably combining two or more stacks of multilayer dielectric thin films with appropriately adjusted thicknesses

Important diseases of cotton in North India and their management HINDI ARTICLE

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