45 research outputs found
Brasiliens politische Dauerkrise: Nach dem kalten Putsch gegen Dilma Rousseff kehrt keine Ruhe ein
Standardized determination of real-time PCR efficiency from a single reaction set-up
We propose a computing method for the estimation of real-time PCR amplification efficiency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground fluorescence phase, non-exponential and plateau phase were excluded from the calculation process by separate mathematical algorithms. We validated the method on experimental data on multiple targets obtained on the LightCycler platform. The developed method yields results of higher accuracy than the currently used method of serial dilutions for amplification efficiency estimation. The single reaction set-up estimation is sensitive to differences in starting concentrations of the target sequence in samples. Furthermore, it resists the subjective influence of researchers, and the estimation can therefore be fully instrumentalized
âFighting mad to tell her storyâ: Madness, rage and literary self-making in Jean Rhys and Jamaica Kincaid
This essay explores the ways that Rhys and Kincaid mine the slippage in meanings and registers of âmadâ and âmadnessâ in their texts. Rather than reading Jean Rhys as primarily the chronicler of alienation and âterrified (white-Creole) consciousness,â I read across her autobiographical and fictional works to argue that âmadâ is deployed in an energizing and critically reflective manner that suggests a more varied and nuanced attunement to the cultural and affective legacies of colonialism. Jamaica Kincaid, for her part, is frequently described as a writer propelled by fury, whose signature affect might without controversy be designated as âanger.â I argue that this emphasis on âangerâ elides the other more profoundly unsettling resonances of âmadâ that inform Kincaidâs oeuvre, as part of her wider project of questioning established repertoires of feeling (including âangerâ and ârageâ). The persistence with which autobiographical elements feature in both writersâ works attests less to a desire for âself-expressionâ than to a painstaking commitment to writing as a space for exploring the very possibility of selfhood. Drawing on selected works by Frantz Fanon and Judith Butler, I navigate across their respective arguments about colonial patriarchal hegemonies as a violent âdisintegrationâ or âundoingâ of the gendered, colonized âselfâ and consider the political and cultural implications of their insights for a reading of Rhysâs and Kincaidâs texts. Focusing on Rhysâs Smile Please: An Unfinished Autobiography (1979), âThe Day They Burned the Booksâ (1968) and Wide Sargasso Sea (1966) alongside Kincaidâs My Brother (1998) and See Now Then (2013) as well as selected interviews, I suggest that reading these two writers in tandem allows us to nuance the meanings that have accrued to âmadâ and âmadnessâ in Caribbean literary studies. It also allows questions of authorship, authority, autobiography and gender to more fully inform prevailing understandings of âmadnessâ in Caribbean literary culture
Standardized determination of real-time PCR efÂźciency from a single reaction set-up
We propose a computing method for the estimation of real-time PCR ampliÂźcation efÂźciency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground ÂŻuorescence phase, nonexponential and plateau phase were excluded from the calculation process by separate mathematical algorithms. We validated the method on experimental data on multiple targets obtained on the LightCycler platform. The developed method yields results of higher accuracy than the currently used method of serial dilutions for ampliÂźcation efÂźciency estimation. The single reaction set-up estimation is sensitive to differences in starting concentrations of the target sequence in samples. Furthermore, it resists the subjective inÂŻuence of researchers, and the estimation can therefore be fully instrumentalized