The effect of vaccine-induced CD8 responses targeting conserved regions on <i>in vitro</i> virus inhibition.

<p>A) box whisker plot comparing the average capacity to inhibit virus replication when targeted sequence is conserved, a Mann-Whitney test for unmatched pairs was used to determine that virus inhibition was significantly higher when vaccines recognized a putative epitope conserved within the relevant virus B) a box whisker plot illustrating that vaccines inhibiting viruses to highest levels recognize epitopes significantly more conserved than those inhibited to lesser levels (Mann Whitney t-test). C) Linear regression analysis relating average conservation of putative epitopes targeted to average inhibition of individual viruses top, IIIB virus is encircled. There is a slight trend towards inhibition of viruses where targeted putative epitopes were more conserved, IIIB is encircled as an outlier (left panel). Where IIIB is excluded (right panel) trend becomes more pronounced and approaches significance.</p

Breadth of peptides recognized following vaccination with Ad35-GRIN and Ad35-ENV.

<p>Breadth of peptides recognized following vaccination with Ad35-GRIN and Ad35-ENV.</p

Reactive peptides inducing responses in CD8 populations ascertained through ICS and ELISpot using expanded CD8 populations, where possible peptides are associated with predefined optimal epitopes using vaccinee HLA.

<p>Bold sequences appear more than once and indicate a preferential immune targeting.</p

Breadth of insert-specific T-cell responses.

<p>Elispot peptide matrices were used to determine the number of possible epitopes recognized by vaccinees. Vaccinees (classified by Volunteer IDs) are stratified by dose and vaccine regimen.</p