Sulphate-reducing bacteria in bovine faeces

Sulphate-reducing bacteria (SRB) were found in all of 200 bovine faeces examined. The number of SRB in bovine faeces ranged from 5 x 10(2) to 6 x 10(8) bacteria g(-1). Of 50 isolates identified, all were assigned to the genus Desulfovibrio

Piyodermalı köpeklerden izole edilen Staphylococcus intermedius’larda eksfoliatif toksin varlığının moleküler olarak belirlenmesi

Staphylococcus aureus ve S. intermedius’un köpeklerde deri enfeksiyonlarına neden olan en önemli türler olduğu kabul edilmektedir. Bu çalışmada, S. intermedius’un eksfoliatif toksinini kodlayan siet (S. intermedius eksfoliatif toksin) genini belirlemek için bir Polimeraz Zincir Reaksiyonu (PCR) tekniği geliştirilmesi ve piyodermalı köpeklerden izole edilen S. intermedius izolatlarında bu genin araştırılması amaçlandı. Piyodermalı köpeklerden izole edilen toplam 41 izolat (35 S. intermedius, 4 S. aureus, bir S. capitis subsp. ureolytica ve bir S. chromogenes) çalışmaya dahil edildi. Bu çalışmada, siet geninin 145 bp’lik ve S. intermedius’un agr (accessory gene regulator) geninin 182 bp’lik bölümünü spesifik olarak çoğaltan özgün primerler tasarlandı. agr geni diğer stafilokok türlerinde değil sadece S. intermedius izolatlarında tespit edildi. Bütün S. intermedius’larda siet geni saptandı. Diğer kontrol suşlarında ve siet negatif S. intermedius suşunda amplifikasyon görülmediği için siet PCR yöntemi spesifik bulundu. Köpeklerde deri lezyonu oluşturan stafilokokların ve bu stafilokokların virulens özelliklerinden siet geninin hızlı ve güvenilir olarak belirlenmesi, tedavi ve korunma anlamında hastalıkla daha etkili mücadele için klinik olarak önemli bilgiler sunacaktır.Staphylococcus aureus and S. intermedius are considered as the most significant species causing skin infections in dogs. The aim of this study was to develop a Polymerase Chain Reaction technique for the detection of siet (S. intermedius exfoliative toxin) gene encoding exfoliative toxin in S. intermedius and to investigate its presence in S. intermedius isolates from dogs with pyoderma. A total of 41 isolates (35 S. intermedius, 4 S. aureus, one S. capitis subsp. ureolytica and one S. chromogenes) from dogs with pyoderma were included in the study. Original primers specifically amplifying 145 bp of siet gene and 182 bp of agr (accessory gene regulator) gene locus of S. intermedius were designed in the study. agr gene was detected in all S. intermedius isolates, but not in other isolates. siet gene was detected in all S. intermedius isolates. siet Polymerase Chain Reaction assay was found to be specific since no amplifications were observed with siet negative S. intermedius and other bacterial control strains. Rapid and reliable detection of staphylococci causing skin lesions in dogs and their virulence markers like siet gene will provide important data for clinical practice to manage the disease more effectively by means of treatment and prevention

Türkiye’deki barınak köpeklerinden izole edilen staphylococcus pseudintermedius’larda Metisilin direnci

Staphylococcus pseudintermedius, stafilokoklar içerisinde yeni tanımlanmış bir tür olup köpeklerden sıklıkla izole edilmektedir. Stafilokoklarda artan antimikrobiyal direnç ve S. pseudintermedius’un enfekte petlerden insanlara geçmesi, halk sağlığını tehdit etmektedir. Bu çalışmada, köpek deri enfeksiyonlarından izole edilen S. pseudintermedius’ların ve metisilin direncinin prevalansının fenotipik ve genotipik metotlar ile belirlenmesi amaçlanmıştır. Toplam 53 köpekten 41 stafilokok izole edildi. Stafilokok izolatlarının 33’ü (%80.4) pta geninin PCR-RFLP ile analiziyle S. pseudintermedius olarak belirlendi. Metisilin direnci kromogenik chromID MRSA agar, oxacillin disk difüzyon metodu ve mecA geninin belirlendiği PCR yöntemi ile bu izolatların 11’inde (%33.3) tespit edildi. Bu çalışma, Türkiye’de metisilin dirençli S. pseudintermedius’ların durumunu gösteren ilk bilimsel rapordur.Staphylococcus pseudintermedius is a recently described species of staphylococci that is frequently isolated from dogs. The increase in antimicrobial resistance in staphylococci and transfer of S. pseudintermedius from infected pets to humans threaten the public health worldwide. In this study, it was aimed to determine the prevalence of S. pseudintermedius from skin infections of dogs and to determine the prevalence of methicillin-resistance in S. pseudintermedius isolates by phenotypic and genotypic methods. A total of 41 staphylococci were isolated from 53 dogs. Thirty three (80.4%) staphylococci were identified as S. pseudintermedius by PCR-RFLP analysis of pta gene. Methicillin resistance was also identified in 11 (33.3%) of these isolates by inoculation on chromogenic chromID MRSA agar, oxacillin disc diffusion method and the determination of mecA gene by PCR. This is the sole report in Turkey that described the situation of methicillin resistant S. pseudintermedius

MS8, Prick of conscience. Image 235

https://repository.wellesley.edu/ms8/1234/thumbnail.jp

The prevalence of major foodborne pathogens in ready-to-eat chicken meat samples sold in retail markets in Turkey and the molecular characterization of the recovered isolates

The aims of the present study were to evaluate the prevalence of Arcobacter spp., Campylobacter spp., Listeria spp., and Salmonella spp. in heat-processed ready-to-eat (RTE) chicken products manufactured by various companies using, bacterial culture methods and to perform virulence gene analysis, serotyping, genotyping, and antibacterial susceptibility tests on the isolated strains. For this purpose, 50 packages of chicken convenience products were used as the study material. Phenotypic tests and a molecular method (Polymerase Chain Reaction, PCR) were used to identify the isolated bacteria. All samples examined were negative for Arcobacter spp., Campylobacter spp., and Salmonella species. Listeria species were isolated from 12 (24%) of the examined samples. Among the Listeria species isolated, 9 were identified as L. monocytogenes, 2 were identified as L. innocua, and one was identified as L. welshimeri. All isolates were susceptible to the antibiotics tested. A detailed molecular analysis of the Listeria spp. revealed that the examined food products posed a significant public health hazard. Considering the presence of different genotypes of L. monocytogenes in RTE food production facilities, all the steps of food production must be reviewed in terms of conformity with sanitation and hygiene rules, and necessary measures must be set in place. (C) 2017 Elsevier Ltd. All rights reserved

Pathogenicity, genotyping and antibacterial susceptibility of the Listeria spp. recovered from stray dogs

The present study aimed to determine the prevalence of Listeria spp. in stray dogs in the Kayseri province of Turkey. In addition, serotyping, genotyping and virulence gene analysis of the isolated Listeria spp. were performed and their pathogenicity and antibacterial susceptibility were investigated

Bacterial and fungal communities in chronic rhinosinusitis with nasal polyps.

ObjectiveMultiple inflammatory mechanisms dynamically interact in the development of chronic rhinosinusitis with nasal polyps (CRSwNP). Disruption of the relationship between host and environmental factors on the mucosal surface leads to the development of inflammation. Microorganisms constitute the most important part of environmental factors.Methods28 volunteers (18 CRSwNP patients and 10 healthy individuals) were included in the study. Eight patients were recurrent nasal polyposis cases, and the remaining were primary cases. Swab samples were taken from the middle meatus under endoscopic examination from all participants. After DNA extraction, a library was created with the Swift Amplicon 16S + ITS kit and sequenced with Illumina Miseq. Sequence analysis was performed using QIIME, UNITE v8.2 database for ITS and Silva v138 for 16S rRNA.ResultsThe predominant bacteria in all groups were Firmicutes, Proteobacteria, Actinobacteria as phyla and Staphylococcus, Corynebacterium, Sphingomonas as genera. Comparison of bacterial communities of CRSwNP patients and control group highlighted Corynebacterium, as the differentiating taxa for control group and Streptococcus, Moraxella, Rothia, Micrococcus, Gemella, and Prevotella for CRSwNP patients. The predominant fungal genus in all groups was Malassezia. Staphylococcus; showed a statistically significant negative correlation with Dolosigranulum. Corynebacterium had a positive correlation with Anaerococcus, and a negative correlation with Neisseria, Prevotella, Fusobacterium and Peptostreptococcus.ConclusionNasal microbiome of CRSwNP patients shows greater inter-individual variation than the control group. Corynebacterium is less abundant in patients with CRSwNP compared to the control group. Malassezia is the predominant fungus in the nasal cavity and paranasal sinuses and correlates positively with the abundance of Corynebacterium