Water Resources Systems Planning and Management: An Introduction to Methods, Models and Applications

This 2005 version has been superseded by the 2017 edition, available in full here: http://hdl.handle.net/1813/48159Throughout history much of the world has witnessed ever-greater demands for reliable, high-quality and inexpensive water supplies for domestic consumption, agriculture and industry. In recent decades there have also been increasing demands for hydrological regimes that support healthy and diverse ecosystems, provide for water-based recreational activities, reduce if not prevent floods and droughts, and in some cases, provide for the production of hydropower and ensure water levels adequate for ship navigation. Water managers are challenged to meet these multiple and often conflicting demands. At the same time, public stakeholder interest groups have shown an increasing desire to take part in the water resources development and management decision making process. Added to all these management challenges are the uncertainties of natural water supplies and demands due to changes in our climate, changes in people's standards of living, changes in watershed land uses and changes in technology. How can managers develop, or redevelop and restore, and then manage water resources systems - systems ranging from small watersheds to those encompassing large river basins and coastal zones - in a way that meets society's changing objectives and goals? In other words, how can water resources systems become more integrated and sustainable

Determining the structure of the bacterial voltage-gated sodium channel NaChBac embedded in liposomes by cryo electron tomography and subtomogram averaging

Voltage-gated sodium channels shape action potentials that propagate signals along cells. When the membrane potential reaches a certain threshold, the channels open and allow sodium ions to flow through the membrane depolarizing it, followed by the deactivation of the channels. Opening and closing of the channels is important for cellular signalling and regulates various physiological processes in muscles, heart and brain. Mechanistic insights into the voltage-gated channels are difficult to achieve as the proteins are typically extracted from membranes for structural analysis which results in the loss of the transmembrane potential that regulates their activity. Here, we report the structural analysis of a bacterial voltage-gated sodium channel, NaChBac, reconstituted in liposomes under an electrochemical gradient by cryo electron tomography and subtomogram averaging. We show that the small channel, most of the residues of which are embedded in the membrane, can be localized using a genetically fused GFP. GFP can aid the initial alignment to an average resulting in a correct structure, but does not help for the final refinement. At a moderate resolution of ˜16 Å the structure of NaChBac in an unrestricted membrane bilayer is 10% wider than the structure of the purified protein previously solved in nanodiscs, suggesting the potential movement of the peripheral voltage-sensing domains. Our study explores the limits of structural analysis of membrane proteins in membranes

Medically Unexplained Oropharyngeal Dysphagia at the University Hospital ENT Outpatient Clinic for Dysphagia: A Cross-Sectional Cohort Study

Medically unexplained oropharyngeal dysphagia (MUNOD) is a rare condition. It presents without demonstrable abnormalities in the anatomy of the upper aero-digestive tract and/or swallowing physiology. This study investigates whether MUNOD is related to affective or other psychiatric conditions. The study included patients with dysphagic complaints who had no detectible structural or physiological abnormalities upon swallowing examination. Patients with any underlying disease or disorder that could explain the oropharyngeal dysphagia were excluded. All patients underwent a standardized examination protocol, with FEES examination, the Hospital Anxiety and Depression Scale (HADS), and the Dysphagia Severity Scale (DSS). Two blinded judges scored five different FEES variables. None of the 14 patients included in this study showed any structural or physiological abnormalities during FEES examination. However, the majority did show abnormal piecemeal deglutition, which could be a symptom of MUNOD. Six patients (42.8%) had clinically relevant symptoms of anxiety and/or depression. The DSS scores did not differ significantly between patients with and without affective symptoms. Affective symptoms are common in patients with MUNOD, and their psychiatric conditions could possibly be related to their swallowing problems

Structure of the merozoite surface protein 1 from Plasmodium falciparum

The merozoite surface protein 1 (MSP-1) is the most abundant protein on the surface of the erythrocyte-invading Plasmodium merozoite, the causative agent of malaria. MSP-1 is essential for merozoite formation, entry into and escape from erythrocytes, and is a promising vaccine candidate. Here, we present monomeric and dimeric structures of full-length MSP-1. MSP-1 adopts an unusual fold with a large central cavity. Its fold includes several coiled-coils and shows structural homology to proteins associated with membrane and cytoskeleton interactions. MSP-1 formed dimers through these domains in a concentration-dependent manner. Dimerization is affected by the presence of the erythrocyte cytoskeleton protein spectrin, which may compete for the dimerization interface. Our work provides structural insights into the possible mode of interaction of MSP-1 with erythrocytes and establishes a framework for future investigations into the role of MSP-1 in Plasmodium infection and immunity

Water Resources Systems Planning and Management: An Introduction to Methods, Models and Applications

This revised, updated textbook presents a systems approach to the planning, management, and operation of water resources infrastructure in the environment. Previously published in 2005 by UNESCO and Deltares (Delft Hydraulics at the time), this new edition, written again with contributions from Jery R. Stedinger, Jozef P. M. Dijkman, and Monique T. Villars, is aimed equally at students and professionals. It introduces readers to the concept of viewing issues involving water resources as a system of multiple interacting components and scales. It offers guidelines for initiating and carrying out water resource system planning and management projects. It introduces alternative optimization, simulation, and statistical methods useful for project identification, design, siting, operation and evaluation and for studying post-planning issues. The authors cover both basin-wide and urban water issues and present ways of identifying and evaluating alternatives for addressing multiple-purpose and multi-objective water quantity and quality management challenges. Reinforced with cases studies, exercises, and media supplements throughout, the text is ideal for upper-level undergraduate and graduate courses in water resource planning and management as well as for practicing planners and engineers in the field.Deltares, UNESCO-IHE Institute for Water Educatio

Action-Evolution Petri Nets:a Framework for Modeling and Solving Dynamic Task Assignment Problems

Dynamic task assignment involves assigning arriving tasks to a limited number of resources in order to minimize the overall cost of the assignments. To achieve optimal task assignment, it is necessary to model the assignment problem first. While there exist separate formalisms, specifically Markov Decision Processes and (Colored) Petri Nets, to model, execute, and solve different aspects of the problem, there is no integrated modeling technique. To address this gap, this paper proposes Action-Evolution Petri Nets (A-E PN) as a framework for modeling and solving dynamic task assignment problems. A-E PN provides a unified modeling technique that can represent all elements of dynamic task assignment problems. Moreover, A-E PN models are executable, which means they can be used to learn close-to-optimal assignment policies through Reinforcement Learning (RL) without additional modeling effort. To evaluate the framework, we define a taxonomy of archetypical assignment problems. We show for three cases that A-E PN can be used to learn close-to-optimal assignment policies. Our results suggest that A-E PN can be used to model and solve a broad range of dynamic task assignment problems.</p