1 research outputs found
Combining a Ru(II) “Building Block” and Rapid Screening Approach to Identify DNA Structure-Selective “Light Switch” Compounds
A chemically
reactive Ru(II) “building block”, able
to undergo condensation reactions with substituted diamines, was utilized
to create a small library of luminescent “light switch”
dipyrido-[3,2-<i>a</i>:2′,3′-<i>c</i>] phenazine (dppz) complexes. The impact of substituent identity,
position, and the number of substituents on the light switch effect
was investigated. An unbiased, parallel screening approach was used
to evaluate the selectivity of the compounds for a variety of different
biomolecules, including protein, nucleosides, single stranded DNA,
duplex DNA, triplex DNA, and G-quadruplex DNA. Combining these two
approaches allowed for the identification of hit molecules that showed
different selectivities for biologically relevant DNA structures,
particularly triplex and quadruplex DNA