2 research outputs found
Shotgun Label-Free Quantitative Proteomics of Water-Deficit-Stressed Midmature Peanut (<i>Arachis hypogaea</i> L.) Seed
Legume seeds and peanuts, in particular,
are an inexpensive source
of plant proteins and edible oil. A comprehensive understanding of
seed metabolism and the effects of water-deficit stress on the incorporation
of the main storage reserves in seeds, such as proteins, fatty acids,
starch, and secondary metabolites, will enhance our ability to improve
seed quality and yield through molecular breeding programs. In the
present study, we employed a label-free quantitative proteomics approach
to study the functional proteins altered in the midmature (65–70
days postanthesis) peanut seed grown under water-deficit stress conditions.
We created a pod-specific proteome database and identified 93 nonredundant,
statistically significant, and differentially expressed proteins between
well-watered and drought-stressed seeds. Mapping of these differential
proteins revealed three candidate biological pathways (glycolysis,
sucrose and starch metabolism, and fatty acid metabolism) that were
significantly altered due to water-deficit stress. Differential accumulation
of proteins from these pathways provides insight into the molecular
mechanisms underlying the observed physiological changes, which include
reductions in pod yield and biomass, reduced germination, reduced
vigor, decreased seed membrane integrity, increase in storage proteins,
and decreased total fatty acid content. Some of the proteins encoding
rate limiting enzymes of biosynthetic pathways could be utilized by
breeders to improve peanut seed production during water-deficit conditions
in the field. The data have been deposited to the ProteomeXchange
with identifier PXD000308
Shotgun Label-Free Quantitative Proteomics of Water-Deficit-Stressed Midmature Peanut (<i>Arachis hypogaea</i> L.) Seed
Legume seeds and peanuts, in particular,
are an inexpensive source
of plant proteins and edible oil. A comprehensive understanding of
seed metabolism and the effects of water-deficit stress on the incorporation
of the main storage reserves in seeds, such as proteins, fatty acids,
starch, and secondary metabolites, will enhance our ability to improve
seed quality and yield through molecular breeding programs. In the
present study, we employed a label-free quantitative proteomics approach
to study the functional proteins altered in the midmature (65–70
days postanthesis) peanut seed grown under water-deficit stress conditions.
We created a pod-specific proteome database and identified 93 nonredundant,
statistically significant, and differentially expressed proteins between
well-watered and drought-stressed seeds. Mapping of these differential
proteins revealed three candidate biological pathways (glycolysis,
sucrose and starch metabolism, and fatty acid metabolism) that were
significantly altered due to water-deficit stress. Differential accumulation
of proteins from these pathways provides insight into the molecular
mechanisms underlying the observed physiological changes, which include
reductions in pod yield and biomass, reduced germination, reduced
vigor, decreased seed membrane integrity, increase in storage proteins,
and decreased total fatty acid content. Some of the proteins encoding
rate limiting enzymes of biosynthetic pathways could be utilized by
breeders to improve peanut seed production during water-deficit conditions
in the field. The data have been deposited to the ProteomeXchange
with identifier PXD000308