SNP primer sequences, melting points (T_m), binding positions on DNA and NCBI accession numbers.

<p>SNP primer sequences, melting points (T_m), binding positions on DNA and NCBI accession numbers.</p

Sequence of SNP2935685 showing a “C” (highlighted in yellow) for aDNA extracted from Croatian skeletal sample 2A (rhino-max).

<p>Sequence of SNP2935685 showing a “C” (highlighted in yellow) for aDNA extracted from Croatian skeletal sample 2A (rhino-max).</p

Sequence of SNP 14676 showing a “C” (highlighted in yellow) for aDNA extracted from St John's Timber Hill skeletal sample 11784 (rhino-max).

<p>Sequence of SNP 14676 showing a “C” (highlighted in yellow) for aDNA extracted from St John's Timber Hill skeletal sample 11784 (rhino-max).</p

RLEP primer sequences, melting temperatures, binding positions on DNA and NCBI accession numbers.

a<p>F, forward; R, reverse.</p

3% agarose gel showing clear bands for the aDNA extracts taken from sample G483, 2A and 3A, matching the size of the <i>M. leprae</i> positive control DNA following amplification with RLEP primers 2 & 4 (111 bp), Croatian samples 1A and 4A did not show a matching band and were not analysed further.

<p><i>Key: 1. M. lep pos control DNA. 2. 2A (Croatia). 3. 3A (Croatia). 4. 4A (Croatia). 5. 1A (Croatia). 6. Extraction blank 1. 7. Extraction blank 2. 8. Water control 1. 9. Water control 2 (run with positive control). 10. G483 (Denmark). 11. 100 bp ladder.</i> N.B. The <i>M. leprae</i> positive control DNA (lane 1) was amplified separately from the extractions to avoid contamination.</p

Skeleton sample information, location, period and burial dates.

<p>Skeleton sample information, location, period and burial dates.</p

3% agarose gel stained with ethidium bromide visualising the reproduction of amplification of aDNA extraction from skeletal UK samples 11784, 11287 and 11503, following amplification with SNP-2935685 (151 bp).

<p>1. Sample 11784. 2. Blank well. 3. Sample 11287. 4. Blank well. 5. Sample 11503. 6. Extraction blank. 7. <i>M. leprae</i> control DNA. 8. Negative water control. 9. 100 bp ladder.</p

The proportion of individuals with a co-morbidity and the number of episodes of ENL.

<p>The proportion of individuals with a co-morbidity and the number of episodes of ENL.</p

Cause of mortality in ENL patients.

<p>Cause of mortality in ENL patients.</p

Co-morbidity associated with ENL.

<p>Co-morbidity associated with ENL.</p