Splenectomy Normalizes Hematocrit in Murine Polycythemia Vera

Splenic enlargement (splenomegaly) develops in numerous disease states, although a specific pathogenic role for the spleen has rarely been described. In polycythemia vera (PV), an activating mutation in Janus kinase 2 (JAK2V617) induces splenomegaly and an increase in hematocrit. Splenectomy is sparingly performed in patients with PV, however, due to surgical complications. Thus, the role of the spleen in the pathogenesis of human PV remains unknown. We specifically tested the role of the spleen in the pathogenesis of PV by performing either sham (SH) or splenectomy (SPL) surgeries in a murine model of JAK2V617F-driven PV. Compared to SH-operated mice, which rapidly develop high hematocrits after JAK2V617F transplantation, SPL mice completely fail to develop this phenotype. Disease burden (JAK2V617) is equivalent in the bone marrow of SH and SPL mice, however, and both groups develop fibrosis and osteosclerosis. If SPL is performed after PV is established, hematocrit rapidly declines to normal even though myelofibrosis and osteosclerosis again develop independently in the bone marrow. In contrast, SPL only blunts hematocrit elevation in secondary, erythropoietin-induced polycythemia. We conclude that the spleen is required for an elevated hematocrit in murine, JAK2V617F-driven PV, and propose that this phenotype of PV may require a specific interaction between mutant cells and the spleen

Rethinking Placements Under Revised Rules & Retrenchment: Successful (and Joyful!) Practicum Partnership Models

This session addresses the new reality facing so many law schools: more demands for experiential learning and fewer resources to dedicate to such courses. We will explore the new era of changing ABA Standards and how to meet the challenge through “practicum” courses, which work to close the access to justice gap, provide students with rich service learning experiences, and forge community partnerships. We as presenters, a clinical professor and a legal writing professor, will share our experiences in this new space and ask others to share theirs. We will contextualize these ideas by sharing several thriving practicum models, which are embedded field experiences created with community partnerships and designed to include a rigorous academic component with an applied-writing requirement. The session will include active participant engagement and bring discourse and sharing in from the very beginning. We hope for a robust discussion of this new model and the complexity of labeling experiential learning under the AALS Section on Clinical Legal Education Glossary for Experiential Education (https://www.aals.org/wp-content/uploads/2017/05/AALS-policy-Vocabulary-l...). The Glossary in some ways begins (and begs) a conversation. We will add to that conversation by exploring how success and joy can emerge from new designs for experiential courses. This concurrent session will also include feedback from community partners who have been working with such models or who have related ideas. This approach will enrich the discussion beyond the primary lens situated within legal education. Participants will leave with shared ideas as well as a handout summarizing related courses following this model. A final version of the handout, reflecting the work of the session, will be available after Externships 9

The multichannel experimental and theoretical study of the 12^{12}C(18^{18}O,18^{18}F)12^{12}B single charge exchange reaction mechanism

International audienceThe study of a network of nuclear reactions populated in the 18O + 12C collision is the main topic of the present paper. It was performed to test nuclear structure and reaction theories in describing the full reaction mechanism occurring in the (18O, 18F) single charge exchange nuclear reaction. From the experimental side, an 18O beam was produced at 275 MeV incident energy by the K800 superconducting cyclotron and the MAGNEX magnetic spectrometer was used at the Laboratori Nazionali del Sud of the Istituto Nazionale di Fisica Nucleare to momentum analyse the ejectiles produced in the nuclear reactions within the same experimental setup. From the theoretical side, the proposed approach consists of analysing the whole network of nuclear reactions in the framework of a unique comprehensive and coherent theoretical calculation. This holistic approach, applied both to the experimental and theoretical analysis, is the main feature and novelty of the work presented here