The Ursinus Weekly, November 22, 1965

Who\u27s Who Among Students honors fourteen seniors: Selection based on scholarship, leadership and potential • Dr. Lissfelt addresses PSEA at first meeting • Susan Starr concert Monday, November 29: Program includes Tchaikovsky and Beethoven • Library consultant on campus Dec. 1 • Ursinus receives $1,600 grant from Sears-Roebuck • Mormon speaker scheduled for Wismer forum • 800 enroll in Ursinus College evening school • Dissinger speaks on olfactory experiments • Philadelphia Art Alliance sponsors poetry contest • Editorial: For support and entertainment; A time to give thanks; Harvest dance • New cafeteria-style breakfast revolutionizes UC eating habits • Letters to the editor • Needed: more fun • Thanksgiving meditation • Team prepares for upcoming debating season • Intramural corner • Cross country • College hockey • Ursinus staggers F&M: D\u27Achille, Kamela lead Bears to 35-13 victory • F&M soccer shades Bears • Greek gleaningshttps://digitalcommons.ursinus.edu/weekly/1211/thumbnail.jp

The Lantern Vol. 35, No. 1, Winter 1969

• Industrialization • Convention • 86 Prof • Even Your Roommate • Specificity • Bo Jangles and Snowstorms in America • You Might Be • Election Night 1968 • Haiku • The Staff of Life • Wind • Brown Mills Blues • The Reunion • Ballad of the Lost Widow • Sunset • You - Revealed • Boredom? • Victim • I Owned A Tree • Days Bounce Along • Oblivion • Realityhttps://digitalcommons.ursinus.edu/lantern/1094/thumbnail.jp

The Lantern Vol. 35, No. 2, Spring 1969

• My Steps Alone • He Said He Hated Time • Communication • Evolution • Morning Almost Faded • Today I Looked • Gloucester • Have You Ever Tried • It\u27s Contact • The Galloping Camel • Life\u27s Flight • Haiku • Today is Monday • At the Waterfront • He Said to Me • Somewhere • A Darkened Window • The Stomach of the Sea • And When I Looked There Were Pinprick Holes in the Sky • Trinity • You Are a Goof • In Transit • Night Will Fall • Praise Be To...?https://digitalcommons.ursinus.edu/lantern/1095/thumbnail.jp

The Lantern Vol. 34, No. 2, May 1968

• The Man Without a System • A Medal for Malcolm • On Hearing That Tonya Will Be Married • The Black Sea • Odyssey \u2767 • Second Poem to Chris • Singularity • Period 5-A Began • Long and Aching Ride • Souvenirs • My Eschatological Epitaph • Discotheque • Some Borrowed Words • False Breakthrough • Shore Morning • The Beholder • Thursday Childless • A Most Prominent Role • It Ran Out • Shades of the Living • The Dark Night of the Mind II • One Step Beyond the Doors • A Note of Thanks to My Parents and Teachers • To a Dead Hippie • A Scrap • Love • Haiku No. 30 • Rachel • There Is No Present • Winter Woods • One Hundred Per Cent Genuine • Heaven • Silence Is Like God • I Soaked Up Silence • Opened Letter From Whistler Homer, Insaned Assailant • Sol Clutch Rides Tonight • I Have Seen Destruction • Upon That Night • That\u27s Weird • Alone • Kathy\u27s Tune • On Walking Home • The Wheel • Some Excuse, at Least • Freedom to Flap • Awareness • Okay, You Guys • You Say You Dream • Bacci Miahttps://digitalcommons.ursinus.edu/lantern/1093/thumbnail.jp

Fatal cytochrome-C oxidase deficient myopathy of infancy associated with mt-DNA depletion: Differential involvement of skeletal muscle and cultured fibroblasts

info:eu-repo/semantics/publishe

Blue native polyacrylamide gel electrophoresis: A powerful tool in diagnosis of oxidative phosphorylation defects

Catalytic activity of oxidative phosphorylation complexes is maintained following separation by Blue Native polyacrylamide gel electrophoresis (BN-PAGE). In BN-PAGE gels, using histochemical staining methods, we have demonstrated enzymatic activity of the complexes I, II, IV, and V in heart and skeletal muscle, liver, and cultured skin fibroblasts. The combination of BN-PAGE and catalytic staining can be successfully applied for detection of complex deficiencies. Tissues from 18 patients with deficiency in the oxidative phosphorylation as detected by spectrophotometric assay were used (10 patients complex IV, three patients complex I, one patient complex H, one patient complex I+III, three patients complex I+IV). The gene defect was located in nuclear DNA in five patients and mitochondrial. DNA in one patient. In samples from patients with a severe deficiency, almost complete absence of the corresponding enzyme band is observed after catalytic staining in the gel. In patients with known partial deficiency, a milder decrease of the corresponding enzyme band is demonstrated. The amount of protein in complexes I, V, and III can easily be evaluated in samples from heart and skeletal muscle after separation by BN-PAGE using silver or Coomassie staining. The protein amount in complex IV is difficult to visualize by silver staining but easier by the Coomassie technique. In samples from liver and cultured skin fibroblasts, evaluation of protein amount is more difficult due to high background staining. In these tissues, immunoblotting can be done after BN-PAGE and subsequent transfer to a nitrocellulose membrane

Diagnostic value of immunostaining in cultured skin fibroblasts from patients with oxidative phosphorylation defects

In the last decades, a large variety of oxidative phosphorylation (OXPHOS) defects have been reported, expressed as an increasing variety of clinical phenotypes. With the expanding number of genes and proteins involved, new screening techniques leading to more effective diagnostic routes are in ever-increasing demand. Cultured skin fibroblasts from a cohort of patients with various OXPHOS defects, previously recognized by enzyme activity studies and blue native PAGE, were investigated with an immunocytochemical technique. Cytospins of cultured fibroblasts were air dried, fixed, and stained with antibodies specifically directed against subunits of each OXPHOS complex. Control cells stained homogeneously and strongly. In fibroblasts from five out of seven patients with a severe deficiency of one of the OXPHOS complexes, a homogeneous reduction of cytoimmunoreactivity of the affected complex was observed. In five out of seven fibroblast strains harboring a mitochondrial tRNA mutation, a mosaic pattern of staining was observed for both complexes I and IV, reflecting the heteroplasmic nature of the defect. The proportion of deficient fibroblasts varied considerably between cell strains from different subjects. The method described offers a convenient and rapid approach to first-line screening of OXPHOS defects. In association with routine assays of enzyme activity, the technique is helpful in orienting molecular investigation further

Fluorescence imaging of mitochondria in cultured skin fibroblasts: a useful method for detection of oxidative phosphorylation defects

BACKGROUND: Protons are pumped from the mitochondrial matrix via oxidative phosphorylation (OXPHOS) into the intermembrane space, creating an electric membrane potential (Delta psi) that is used for adenosine triphosphate (ATP) production. Defects in one or more of the OXPHOS complexes are associated with a variety of clinical symptoms, often making it difficult to pinpoint the causal mutation. METHODS: In this article, a microscopic method for the quantitative evaluation of Delta psi in cultured skin fibroblasts is described. The method using 5,5',6,6'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) fluorescence staining was tested in a selection of OXPHOS-deficient cell lines. RESULTS: A significant reduction of Delta psi was found in the cell lines of patients with either an isolated defect in complex I, II, or IV or a combined defect (complex I + complex IV). Delta psi was not reduced in the fibroblasts of two patients with severe complex V deficiency. Addition of the complex I inhibitor rotenone induced a significant reduction of ALP and perinuclear relocalization of the mitochondria. In cells with a heteroplasmic mitochondrial DNA (mtDNA) defect, a more heterogeneous reduction of Delta psi was detected. CONCLUSION: Our data show that imaging of Delta psi in cultured skin fibroblasts is a useful method for the evaluation of OXPHOS functioning in cultured cell lines