Additional file 1: Table S1. of miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development

The shRNA clone and the agomir and antagomir sequences. (DOCX 17 kb

Divinæ scripturæ ueteris noueq omnia

[Bible. A.T.. 1526Version grecque ancienne)][1526]Numérisé par le partenaireAppartient à l’ensemble documentaire : BNUStr012Appartient à l’ensemble documentaire : BNUStras1Numérisé par le partenair

Additional file 5: Table S5. of miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development

Association between HMGA2 mRNA expression and the clinicopathologic characteristics of endometrial cancer patients (n = 40). (DOCX 16 kb

Additional file 4: Table S4. of miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development

S4-i: The expression of HMGA2 protein in normal endometrial tissue (n = 19) and in endometrial carcinoma tissue (n = 80); S4-ii: Association between HMGA2 protein expression and the clinicopathologic characteristics of endometrial cancer patients. (DOCX 16 kb

Sensitive Immunosensor for N‑Terminal Pro-brain Natriuretic Peptide Based on N‑(Aminobutyl)-N-(ethylisoluminol)-Functionalized Gold Nanodots/Multiwalled Carbon Nanotube Electrochemiluminescence Nanointerface

A novel electrochemiluminescence (ECL) immunosensor was developed for the determination of N-terminal pro-brain natriuretic peptide (NT-proBNP) by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI)-functionalized gold nanodots/chitosan/multiwalled carbon nanotubes (ABEI/GNDs/chitosan/COOH-MWCNTs) hybrid as nanointerface. First, ABEI/GNDs/chitosan/COOH-MWCNTs hybrid nanomaterials were grafted onto the surface of ITO electrode via the film-forming property of hybrid nanomaterials. The anti-NT-proBNP antibody was connected to the surface of modified electrode by virtue of amide reaction via glutaraldehyde. The obtained sensing platform showed strong and stable ECL signal. When NT-proBNP was captured by its antibody immobilized on the sensing platform via immunoreaction, the ECL intensity decreased. Direct ECL signal changes were used for the determination of NT-proBNP. The present ECL immunosensor demonstrated a quite wide linear range of 0.01–100 pg/mL. The achieved low detection limit of 3.86 fg/mL was about 3 orders of magnitude lower than that obtained with electrochemistry method reported previously. Because of the simple and fast analysis, high sensitivity and selectivity, and stable and reliable response, the present immunosensor has been successfully applied to quantify NT-proBNP in practical plasma samples. The success of the sensor in this work also confirms that ABEI/GNDs/chitosan/COOH-MWCNTs hybrid is an ideal nanointerface to fabricate a sensing platform. Furthermore, the proposed strategy could be applied in the detection of other clinically important biomarkers

Construction of Injectable Double-Network Hydrogels for Cell Delivery

Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly­(ethylene oxide) formed the first network, while calcium alginate formed the second one, and in the resultant DN hydrogel, either of the networks could be selectively removed. The moduli of the DN hydrogel were significantly improved compared to that of the parent single-network hydrogels and were tunable by changing the chemical components. In situ 3D cell encapsulation could be easily performed by mixing cell suspension to the polymer solutions and transferred through a syringe needle before sol–gel transition. Cell proliferation and mediated differentiation of mouse chondrogenic cells were achieved in the DN hydrogel extracellular matrix

Sex-dependent aortic valve pathology in patients with rheumatic heart disease

<div><p>Background</p><p>Rheumatic heart disease is an autoimmune disease caused by group A streptococci infection and frequently affects the aortic valve. Sex differences are common in the disease progression, treatment, and outcome. However, little is known about the sex differences in the pathology of aortic valves in rheumatic heart disease.</p><p>Design</p><p>We studied the end-stage calcific aortic valves from male versus female patients to reveal the sex-dependent pathology differences and molecular changes associated with requiring valve replacement.</p><p>Methods</p><p>Aortic valves from 39 patients with rheumatic heart disease (19 males and 20 females) were collected at the time of aortic valve replacement for comparative pathology, immunohistochemistry, and gene expression analyses. Clinical characteristics were also analyzed and compared between the two groups.</p><p>Results</p><p>Aortic valves from female patients exhibited increased expression of collagens, infiltration of monocytes/macrophages and neovascularization. Aortic valves from female patients also had increased expression of inflammatory genes involved in the NFKB pathway (phosphorylated NFKB p65 subunit, IL8, and NOS3) and Th1 cytokine genes (IFNA and IL12B). The severe valve pathology in female patients was correlated with a higher serum level of anti-streptolysin O antibodies.</p><p>Conclusion</p><p>Inflammation is more prominent in aortic valves of female patients with rheumatic heart disease. This sex difference may contribute to the severe valve pathology and worse outcome of female patients.</p></div

Anionic Effect of δ and χ Forms of Tris(alkoxo) Ligand Functionalized Anderson-Type Polyoxometalates on Nonlinear Optical Response of Porphyrin–POM Based Supramolecular Compounds

The electrostatic interaction of tris­(alkoxo) ligand functionalized Anderson-type polyoxometalate (POM for short) anions [XMo₆O₁₈{(OCH₂)₃CNH₂}₂]^3– (where X = Mn or Fe) having δ-type structures and [H₂ZnMo₆O₁₈{(OCH₂)₃CNH₂}₂]^2– having χ-type structure with porphyrin-based cation [H₂TPP]²⁺ results in formation of three supramolecular compounds [H₂TPP]_1.5[MnMo₆O₁₈{(OCH₂)₃CNH₂}₂] (<b>1</b>), [H₂TPP]_1.5[FeMo₆O₁₈{(OCH₂)₃CNH₂}₂]·2C₃H₇NO (<b>2</b>), and [H₂TPP]­[H₂ZnMo₆O₁₈{(OCH₂)₃CNH₂}₂]·2­(C₂H₅)₂O (<b>3</b>). All the supramolecular compounds have significant nonlinear reverse saturated absorption (RSA) and self-defocusing behavior especially the compound <b>3</b> has noteworthy improvement of second hyperpolarizability γ value (1.35 × 10^–27 esu) compared to parent porphyrin (7.7 × 10^–28 esu). Fluorescence is quenched by ca. 74% in compound <b>3</b>, 37% in compound <b>1</b> and 30% in compound <b>2</b> compared to that of parent porphyrin, respectively, indicating different quenching effect of the different POM anions. Parallelly, it was found that the three POM anions have different onset reduction potentials due to different central metal atom and overall cluster structure, corresponding to different HOMO–LUMO gaps (<i>E</i>_g) of the resulting supramolecular compounds with <i>E</i>_g (<b>3</b>) <<i>E</i>_g (<b>1</b>) <<i>E</i>_g (<b>2</b>) <<i>E</i>_g (parent porphyrin<b>)</b>. Thus, we thought that the easier electronic charge transfer from porphyrin to POM anions by irradiation with laser light plays a key role in the enhancement of nonlinear optical response in supramolecular compounds

Remarkable Enhancement in the Nonlinear Optical Responses of Porphyrins Realized by Combination with Polyoxometalates via Covalent Bonding: A Case Study

Integrating polyoxometalates into porphyrin moieties via covalent bond is expected to be a new approach to tune and enhance the nonlinear optical responses of porphyrins. The effectiveness and efficiency of this proposal has been examined and witnessed by studying two new hybrid compounds as a prototype, namely, (Bu₄N)₄K₂­[{C₅₂H₃₂N₅O₂Zn}­HNC­(CH₂O)₃­P₂V₃­W₁₅O₅₉]·2­(C₄H₉NO) (<b>1</b>) and (Bu₄N)₄K₂­[{C₅₂H₃₄N₅O₂}­HNC­(CH₂O)₃­P₂V₃­W₁₅O₅₉]·3­(C₄H₉NO) (<b>2</b>), in which porphyrins acting as electron donor and Dawson type polyoxometalate acting as electron acceptor are connected via short tether through covalent bond. The new compounds are systematically characterized by means of elemental analyses, FT-IR, ¹H NMR, ESI-MS, TG/DTA, UV–vis, fluorescence emission spectra, and cyclic voltammetry measurement. Remarkably, great enhancement in reverse saturation absorption is achieved in <b>1</b> and <b>2</b> which is ca. 1 order of magnitude greater than their individual reactants. Additionally, optical-limiting thresholds are obtained being 0.484 J/cm² for <b>1</b> and 0.501 J/cm² for <b>2</b>, respectively, implying their high potential as low-power optical-limiting materials

Clinical characteristics of the RHD patients.

<p>Clinical characteristics of the RHD patients.</p