5 research outputs found
Amphiphilic tricationic Zn(II)phthalocyanine provides effective photodynamic action to eradicate broad-spectrum microorganisms
A novel tricationic Zn(II)phthalocyanine derivative, (NCH3)3ZnPc3+, was synthesized by ring expansion reaction of boron(III) [2,9(10),16(17)-trinitrosubphthalocyaninato]chloride. First, the reaction of this subphthalocyanine with 2,3-naphthalenedicarbonitrile and Zn(CH3COO)2 catalyzed by 8-diazabicyclo[5.4.0]undec-7-ene was used to obtain the A3B-type nitrophthalocyanine. After reduction of nitro groups with Na2S and exhaustive methylation of amino groups, (NCH3)3ZnPc3+ was formed in good yields. In addition, the tetracationic analog (NCH3)4ZnPc4+ was synthesized to compare their properties. The absorption and fluorescence spectra showed the Q-bands and the red emission, respectively, which are characteristic of the Zn(II)phthalocyanine derivatives in N,N-dimethylformamide. Furthermore, photodynamic activity sensitized by these compounds was studied in the presence of different molecular probes to sense the formation of reactive oxygen species. (NCH3)3ZnPc3+ efficiently produced singlet molecular oxygen and also it sensitized the formation of superoxide anion radical in the presence of NADH, while the photodynamic activity of (NCH3)4ZnPc4+ was very poor, possibly due to the partial formation of aggregates. Furthermore, the decomposition of L-tryptophan induced by (NCH3)3ZnPc3+ was mainly mediated by a type II mechanism. Antimicrobial photodynamic inactivation sensitized by these phthalocyanines was evaluated in Staphylococcus aureus, Escherichia coli, and Candida albicans, as representative microbial cells. In cell suspensions, (NCH3)3ZnPc3+ was rapidly bound to microbial cells, showing bioimages with red fluorescence emission. After 5 min of irradiation with visible light, (NCH3)3ZnPc3+ was able to completely eliminate S. aureus, E. coli and C. albicans, using 1.0, 2.5 and 5.0 ΌM phthalocyanine, respectively. In contrast, a low photoinactivation activity was found with (NCH3)4ZnPc4+ as a photosensitizer. Therefore, the amphiphilic tricationic phthalocyanine (NCH3)3ZnPc3+ is a promising photosensitizing structure for application as a broad-spectrum antimicrobial phototherapeutic agent.Fil: Baigorria, EstefanĂa. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Durantini, Javier Esteban. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones en TecnologĂas EnergĂ©ticas y Materiales Avanzados. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones en TecnologĂas EnergĂ©ticas y Materiales Avanzados; ArgentinaFil: Di Palma, MarĂa Albana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; ArgentinaFil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; ArgentinaFil: Milanesio, MarĂa Elisa. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Durantini, Edgardo NĂ©stor. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentin
Photodynamic Action Mechanism Mediated by Zinc(II) 2,9,16,23-Tetrakis[4-(N-methylpyridyloxy)]phthalocyanine in Candida albicans Cells
The photoreaction type I/type II pathways mediated by zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was studied in Candida albicans cells. This photosensitizer was strongly bound to C. albicans cells at short times. After 30 min irradiation, 5 ÎŒM ZnPPc4+ produced ~5 log decrease in cell viability. Different probes were used to detect reactive oxygen species (ROS) in cell suspensions (~106 CFU mL-1). Singlet molecular oxygen, O2(1Îg), was observed by the reaction with 9,10-dimethylanthracene (DMA) and tetrasodium 2,2-(anthracene-9,10-diyl)bis(methylmalonate) (ABMM), whereas the nitro blue tetrazolium (NBT) method was used to sense superoxide anion radical (O2·-). Moreover, the effects produced by an anoxic atmosphere and cell suspensions in D2O, as well as the addition of sodium azide and mannitol as ROS trapping were evaluated in the PDI of C. albicans. These investigation indicates that O2(1Îg) is generated in the cells, although a minor extension other radical species can also be involved in the PDI of C. albicans mediated by ZnPPc4+.Fil: Di Palma, MarĂa Albana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; ArgentinaFil: Alvarez, MarĂa Gabriela. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Durantini, Edgardo NĂ©stor. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentin
Optimization of cellular uptake of zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine for maximal photoinactivation of Candida albicans
Cellular uptake and photodynamic action of zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+) was examined in Candida albicans. In vitro investigations showed that ZnPPc4+ was rapidly bound to C. albicans cells. The binding of phthalocyanine to cells was dependent on ZnPPc4+ concentrations (1â10 ÎŒM) and cells densities (106â108 cells mLâ1). A high amount of ZnPPc4+ retained in the cells after two washing steps, indicating a strong interaction between the photosensitizer and C. albicans. The uptake was temperature dependent, although the difference between 37 °C and 4 °C was about 10 %. Also, the amount of ZnPPc4+ bound to C. albicans was affected when the cells were incubated for a longer time with azide and 2,4-dinitrophenol (DNP) prior to treatment with ZnPPc4+. Cell survival after irradiation was dependent on the irradiation period, ZnPPc4+ concentration and cells density. Photoinactivation of C. albicans cells was elevated even after two washing steps. The strong dependence of uptake on cell density reveals the strength and avidity of the binding of ZnPPc4+ to C. albicans cells. The accumulation behaviour of ZnPPc4+ suggests that mainly an affinity-mediated binding mechanism can be involved. Therefore, ZnPPc4+ is an interesting phthalocyanine for photodynamic inactivation (PDI) of yeasts in liquid suspensions.Fil: Di Palma, MarĂa Albana. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Alvarez, MarĂa Gabriela. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Ochoa, Ana Laura. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Milanesio, MarĂa Elisa. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Durantini, Edgardo NĂ©stor. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de QuĂmica; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentin
Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubationFil: Villasuso, Ana Laura. Universidad Nacional de Rio Cuarto; ArgentinaFil: Di Palma, MarĂa Albana. Universidad Nacional de Rio Cuarto; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Bahia Blanca. Instituto de Investigaciones BioquĂmicas Bahia Blanca (i); ArgentinaFil: PasquarĂ©, Susana J.. Consejo Nacional de Investigaciones Cientificas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Bahia Blanca. Instituto de Investigaciones BioquĂmicas Bahia Blanca (i); ArgentinaFil: Racagni, Graciela. Universidad Nacional de Rio Cuarto; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Bahia Blanca. Instituto de Investigaciones BioquĂmicas Bahia Blanca (i); ArgentinaFil: Machado, Estela E.. Universidad Nacional de Rio Cuarto; Argentin
Improvement of maize yield by foliar application of Azospirillum brasilense Az39
Azospirillum brasilense is a plant growth promoting bacteria (PGPB) widely used as an inoculant in diverse species, including maize (Zea mays L.) to improve their growth, development and productivity. Its ability to survive to the presence of herbicides has been demonstrated. Currently, the effectiveness of foliar application of biological formulations takes advantage of the intrinsic capacity of bacteria, regardless of the edaphic conditions of the soil. The objective of this work was to evaluate the effects of the foliar inoculation of A. brasilense Az39 in maize crop with different combinations of herbicides to increase production and to improve sustainable management practices. The field study was performed during three successive seasons. In the growing seasons between 2015 and 2018, different combinations of glyphosate and atrazine were evaluated with and without the foliar inoculation of Az39. A higher yield and total chlorophyll content were observed, together with a lower damage of membranes in the treatment with foliar inoculation of Az39, one application of atrazine and two applications of glyphosate. The yield varied between seasons but in all of them, the foliar inoculations with Az39 benefited the crop. Therefore, application of A. brasilense Az39 improves the performance of the maize crop under suitable combinations of herbicides based upon cost and environmental risk. These results, obtained with PGPB native strains, demonstrate its complementary benefits for the development of sustainable crop production practices.Fil: Cardozo, Paula Gabriela. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Di Palma, MarĂa Albana. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Martin, Soledad Marianel. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Cerliani, Cecilia. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de AgronomĂa y Veterinaria; ArgentinaFil: EspĂłsito, Gabriel Pablo. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de AgronomĂa y Veterinaria; ArgentinaFil: Reinoso, Herminda Elmira. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Travaglia, Claudia Noemi. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentin