Does postoperative radiation therapy represent a contraindication to expander-implant based immediate breast reconstruction? An update 2012-2014

Post-mastectomy radiotherapy (PMRT) is well known in the plastic surgery community for having a negative impact on expander-implant based immediate breast reconstruction (IBBR), although recently some technical improvements allow better results. Very recent papers would suggest that there is no difference in postoperative complications in patients receiving post-mastectomy radiotherapy using modern techniques. However, study results are often biased by small groups of patients and by heterogeneity of radiotherapy timing, different surgical techniques and measured outcomes

The role of type 4 phosphodiesterases in generating microdomains of cAMP: Large scale stochastic simulations

Cyclic AMP (cAMP) and its main effector Protein Kinase A (PKA) are critical for several aspects of neuronal function including synaptic plasticity. Specificity of synaptic plasticity requires that cAMP activates PKA in a highly localized manner despite the speed with which cAMP diffuses. Two mechanisms have been proposed to produce localized elevations in cAMP, known as microdomains: impeded diffusion, and high phosphodiesterase (PDE) activity. This paper investigates the mechanism of localized cAMP signaling using a computational model of the biochemical network in the HEK293 cell, which is a subset of pathways involved in PKA-dependent synaptic plasticity. This biochemical network includes cAMP production, PKA activation, and cAMP degradation by PDE activity. The model is implemented in NeuroRD: novel, computationally efficient, stochastic reaction-diffusion software, and is constrained by intracellular cAMP dynamics that were determined experimentally by real-time imaging using an Epac-based FRET sensor (H30). The model reproduces the high concentration cAMP microdomain in the submembrane region, distinct from the lower concentration of cAMP in the cytosol. Simulations further demonstrate that generation of the cAMP microdomain requires a pool of PDE4D anchored in the cytosol and also requires PKA-mediated phosphorylation of PDE4D which increases its activity. The microdomain does not require impeded diffusion of cAMP, confirming that barriers are not required for microdomains. The simulations reported here further demonstrate the utility of the new stochastic reaction-diffusion algorithm for exploring signaling pathways in spatially complex structures such as neurons

Proanthocyanidins from \u3cem\u3eHedysarum, Lotus\u3c/em\u3e and \u3cem\u3eOnobrychis\u3c/em\u3e spp. Growing in Sardinia and Sicily and Their Antioxidant Activity

Proanthocyanidins (PA), or condensed tannins, are a class of natural polyphenolic compounds, occurring in numerous plant species, including a number of economically significant forage legumes. These compounds are polymers of flavan-3-ols, and typically contain from 2 to 20 units. Their biological significance is still being debated and, in recent years, a great deal of attention has been focused on their role in ruminant nutrition. Evidence has indicated that PA, in a moderate concentration (0.5-5% DM), may have considerable importance in protecting dietary proteins against microbial degradation in the rumen, and in preventing bloat. The antioxidant activity (AA) is also an important feature for animal well-being (Barry & McNabb, 1999). In order to study the PA content related to the antioxidant activity, samples of Hedysarum, Lotus and Onobrychis spp. from Mediterranean environments have been considered and investigated

Cognitive Radio Networks: A Practical Perspective

Guest Editoria

Synthetic Spectra and Color-Temperature Relations of M Giants

As part of a project to model the integrated spectra and colors of elliptical galaxies through evolutionary synthesis, we have refined our synthetic spectrum calculations of M giants. After critically assessing three effective temperature scales for M giants, we adopted the relation of Dyck et al. (1996) for our models. Using empirical spectra of field M giants as a guide, we then calculated MARCS stellar atmosphere models and SSG synthetic spectra of these cool stars, adjusting the band absorption oscillator strengths of the TiO bands to better reproduce the observational data. The resulting synthetic spectra are found to be in very good agreement with the K-band spectra of stars of the appropriate spectral type taken from Kleinmann & Hall (1986) as well. Spectral types estimated from the strengths of the TiO bands and the depth of the bandhead of CO near 2.3 microns quantitatively confirm that the synthetic spectra are good representations of those of field M giants. The broad-band colors of the models match the field relations of K and early-M giants very well; for late-M giants, differences between the field-star and synthetic colors are probably caused by the omission of spectral lines of VO and water in the spectrum synthesis calculations. Here, we present four grids of K-band bolometric corrections and colors -- Johnson U-V and B-V; Cousins V-R and V-I; Johnson-Glass V-K, J-K and H-K; and CIT/CTIO V-K, J-K, H-K and CO -- for models having 3000 K < Teff < 4000 K and -0.5 < log g < 1.5. These grids, which have [Fe/H] = +0.25, 0.0, -0.5 and -1.0, extend and supplement the color-temperature relations of hotter stars presented in a companion paper (astro-ph/9911367).Comment: To appear in the March 2000 issue of the Astronomical Journal. 60 pages including 15 embedded postscript figures (one page each) and 6 embedded postscript tables (10 pages total

All-suture anchors in arthroscopic acetabular labral repair: our experience

BACKGROUND: Over the past years, the role of the acetabular labrum in hip joint biomechanics and its relations with joint health has been of particular interest. There is a good clinical improvement of patients in whom the acetabular labrum is preserved during arthroscopic hip surgery. The purpose of this study is to report the results of arthroscopic repair of labral tears at a medium term follow up. MATERIALS AND METHODS: We performed a retrospective review of all cases that underwent hip arthroscopy at our Institution from January 2013 until December 2018. There were 24 patients, 13 males and 11 females, and their mean age at the time of surgery was 29, 42 years (range, 19 to 43 years). All patients were treated by the same surgeon with an extracapsular OUT-IN approach. Suture was performed using a non-absorbable suture anchor all-suture. Clinical assessment was performed at December 2019 using a modified Harris hip score (mHHS), hip outcomes score activities of daily living (HOS ADL), hip outcomes score activities of sport scale (HOS SS). All patients with acetabular labrum injury had femoro-acetabular impingement. RESULTS: The mean overall values in the preoperative period were 67.21 \ub1 10.31 for mHHS, 70.04 \ub1 12.11 for HOS-ADL and 60.06 \ub1 14.58 for HOS -SS. The results obtained in the re-evaluations of patients in December 2019 with a mean follow-up of 38, 3 months (minimum 1 year) are on average 82.17 \ub1 11.36 for mHHS, 83.00 \ub1 12.80 for HOS-ADL and 76.09 \ub1 18.52 for HOS-SS. CONCLUSIONS: The progress of knowledge and the advancement of diagnostic and therapeutic skills has led to a greater awareness of the importance of treating acetabular labrum tears. Arthroscopic treatment with suture appear to be a good option for these patients and we had encouraging results in our center

Positioning by multicell fingerprinting in urban NB-IoT networks

Narrowband Internet of Things (NB-IoT) has quickly become a leading technology in the deployment of IoT systems and services, owing to its appealing features in terms of coverage and energy efficiency, as well as compatibility with existing mobile networks. Increasingly, IoT services and applications require location information to be paired with data collected by devices; NB-IoT still lacks, however, reliable positioning methods. Time-based techniques inherited from long-term evolution (LTE) are not yet widely available in existing networks and are expected to perform poorly on NB-IoT signals due to their narrow bandwidth. This investigation proposes a set of strategies for NB-IoT positioning based on fingerprinting that use coverage and radio information from multiple cells. The proposed strategies were evaluated on two large-scale datasets made available under an open-source license that include experimental data from multiple NB-IoT operators in two large cities: Oslo, Norway, and Rome, Italy. Results showed that the proposed strategies, using a combination of coverage and radio information from multiple cells, outperform current state-of-the-art approaches based on single cell fingerprinting, with a minimum average positioning error of about 20 m when using data for a single operator that was consistent across the two datasets vs. about 70 m for the current state-of-the-art approaches. The combination of data from multiple operators and data smoothing further improved positioning accuracy, leading to a minimum average positioning error below 15 m in both urban environments

Microenvironment in neuroblastoma: Isolation and characterization of tumor-derived mesenchymal stromal cells

Background: It has been proposed that mesenchymal stromal cells (MSCs) promote tumor progression by interacting with tumor cells and other stroma cells in the complex network of the tumor microenvironment. We characterized MSCs isolated and expanded from tumor tissues of pediatric patients diagnosed with neuroblastomas (NB-MSCs) to define interactions with the tumor microenvironment. Methods: Specimens were obtained from 7 pediatric patients diagnosed with neuroblastoma (NB). Morphology, immunophenotype, differentiation capacity, proliferative growth, expression of stemness and neural differentiation markers were evaluated. Moreover, the ability of cells to modulate the immune response, i.e. inhibition of phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMCs) and natural killer (NK) cytotoxic function, was examined. Gene expression profiles, known to be related to tumor cell stemness, Wnt pathway activation, epithelial-mesenchymal transition (EMT) and tumor metastasis were also evaluated. Healthy donor bone marrow-derived MSCs (BM-MSC) were employed as controls. Results: NB-MSCs presented the typical MSC morphology and phenotype. They showed a proliferative capacity superimposable to BM-MSCs. Stemness marker expression (Sox2, Nanog, Oct3/4) was comparable to BM-MSCs. NB-MSC in vitro osteogenic and chondrogenic differentiation was similar to BM-MSCs, but NB-MSCs lacked adipogenic differentiation capacity. NB-MSCs reached senescence phases at a median passage of P7 (range, P5-P13). NB-MSCs exhibited greater immunosuppressive capacity on activated T lymphocytes at a 1:2 (MSC: PBMC) ratio compared with BM-MSCs (p = 0.018). NK cytotoxic activity was not influenced by co-culture, either with BM-MSCs or NB-MSCs. Flow-cytometry cell cycle analysis showed that NB-MSCs had an increased number of cells in the G0-G1 phase compared to BM-MSCs. Transcriptomic profiling results indicated that NB-MSCs were enriched with EMT genes compared to BM-MSCs. Conclusions: We characterized the biological features, the immunomodulatory capacity and the gene expression profile of NB-MSCs. The NB-MSC gene expression profile and their functional properties suggest a potential role in promoting tumor escape, invasiveness and metastatic traits of NB cancer cells. A better understanding of the complex mechanisms underlying the interactions between NB cells and NB-derived MSCs should shed new light on potential novel therapeutic approaches