Molecular Detection of Carbapenem Resistance in Clinical Isolates of Klebsiella pneumoniae in Tertiary Care Hospital

Antibiotic resistance has become a serious global threat, mainly due to misuse, overuse of antibiotics and non-compliance with infection control protocol. Superbugs are multidrug-resistant (MDR) and extended drug-resistant (XDR) bacteria, mainly Klebsiella pneumoniae and Escherichia coli from the Enterobacteriaceae family, which cause opportunistic infections and raise death rates and hospital expenditures. The present study was conducted at a tertiary care teaching hospital to study the epidemiology and molecular detection of carbapenem-resistant K. pneumoniae isolated from various clinical specimens. 240 K. pneumoniae isolates were collected from January 2020 to December 2021 at the Bacteriology laboratory, Index Medical College and Hospital, Indore. All isolates were analyzed for carbapenem resistance by the conventional disc diffusion method. All carbapenem-resistant isolates were tested for carbapenemase production using the phenotypic double-disk synergy test (DDST) and modified Hodge test (MHT) as per 2020 CLSI guidelines. All isolates were negative by phenotypic methods, further confirmed by conventional PCR to detect the gene responsible for carbapenemase production. 240 isolates of K. pneumoniae were included during the study periods. Out of 240 isolates, 102 isolates were found resistant to carbapenem drugs. All 102 isolates were confirmed carbapenemase and MBL producers by MHT and DDST tests. Among 102, 60 isolates were found to be MBL producers negative by MHT and DDST tests. Sixty phenotypic negative carbapenem-resistant isolates were tested by conventional PCR. One or more carbapenemase genes were detected in 61.0% of isolates. The blaKPC was detected in 13/60 (21%) isolates, followed by blaNDM 10/60 (16%) isolates, followed by blaVIM in 6/60(10%), blaOXA-48 in 5/60 (8%) and blaIMP in 3/60(5%) isolates. K. pneumoniae produces carbapenemase, which enhances resistance to the carbapenem class of antibiotics. The simultaneous detection of these resistance genes expressed by Klebsiella pneumoniae might be managed by early detection and adhering to antibiotic policies that limit the use of antibiotics

COVID-19: A pandemic situation: A review article

Aim: To include a summary of what has been written so far about COVID-19, enabling access to information and a greater understanding of the pandemic, and to assist the medical community in making decisions about the virus. Armed conflicts and communicable disease natural events are both preventable occurrences that can devastate human societies. Pandemic illnesses, like the COVID-19, are usually highly promulgated within the It's possible that exposure to the media is linked to increased anxiety and compensatory behaviour (e.g., using hand sanitizer). Health anxiety, contamination fears, and disgust sensitivity are all significant predictors of corona link anxiety, according to multivariate analysis. Implications for a new approach to pandemic issues diseases of COVID affected the lives of many folks at personal and social levels. Many of us lost our dear ones, their job security, susceptible to several new pneumonic diseases because the COVID-19 is well-known to damage the lungs permanently. We tend to estimate that early infection of COVID-19 shows exponential growth rates of 38per cent per day [1]

Catalase Test and Gram Staining of Uncentrifuged Urine for the Diagnosis of Urinary Tract Infection: A Cross-sectional Study

Introduction: Urinary Tract Infection (UTI) is caused by the abnormal growth of the pathogen in the urinary tract. Urine Microscopy and culture is still the gold standard for the isolation of bacteria. However, screening tests are cost-effective and more practical in managing UTIs. Aim: To determine rapid screening tests (Gram Staining and Catalase test) compared to culture. Materials and Methods: The cross-sectional study was done in the Department of Microbiology, Jawaharlal Nehru Medical College; Acharya Vinoba Bhave Rural Hospital, Sawangi Meghe, Wardha, Maharashtra, India, for a period of one year from August 2019 to September 2020. In this study, 100 urine samples were processed by screening tests such as the Catalase test and Gram stain, followed by culture. Results: Positive predictive value of the catalase test was 55.31% and Gram’s stain was 78.26%. In contrast, the negative predictive value of catalase was 69.81%, and Gram stain was 88.88%. The sensitivity and specificity of the catalase test was 61.90% and 63.79%, respectively and the sensitivity and specificity of Gram stain was 85.71% and 82.75%, respectively. Conclusion: Gram stain had the highest sensitivity, 85.71%, and specificity of 82.75% compared to the catalase test. Candida spp. was the most frequently isolated from a urine culture, followed by Enterococcus spp. E. coli, and Klebsiella spp. were also commonly isolated from people

Ultraviolet irradiation doses for coronavirus inactivation -review and analysis of coronavirus photo inactivation studies

Background: To slow the increasing global spread of the SARS-CoV-2 virus, appropriate disinfection techniques are required. Ultraviolet radianon (UV) has a well-known antiviral effect. But measurements on the radiation dose necessary to inactivate SARS-CoV-2 have not been pub- lished so far. Methods: Coronavirus inactivation experiments with ultraviolet light performed in the past were evaluated to determine the UV radiation dose required for a 90% virus reduction. This analysis is based on the fact that all coronaviruses have a similar structure and similar RNA strand length. Results: The available data reveals large variations, which are apparently not caused by the coronaviruses but by the experimental conditions selected. If these are excluded as far as possible, it appears that coronaviruses are very UV sensitive. The upper limit determined for the log-reduction dose (90% reduction) is approximately 10.6 mJ/cm2 (median), while the true value is  probably only 3.7 mJ/cm2 (median). Conclusion: Since coronaviruses do not differ structurally to any great exent, the SARS-CoV-2 virus – as well as possible future mutations – will very likely be highly UV sensitive, so that common UV disinfection procedures will inactivate the new SARS-CoV-2 virus without any further modification

Bacteriologic Antibiography Outline of Isolates from Blood Culture at Tertiary Center

Bacteria found in blood circulation either consistently intermittently are commination to every organs of body. These infections can affect life and death. In India also blood stream infections are major causes of health problem that caused demise of patients in hospital. Timely diagnoses of infection with antimicrobial susceptibility assessment are important for optimization of treatment and best way to reduce hospital stay and improve patient health. In spite of recent advances in clinical diagnostics, blood culture remains the gold standard for the detection of blood stream infections. Studies in different places and regions have indicated the varying microbiological pattern of bloodstream infections which support the need for a continuous examination of the causative organisms. For the diagnosis of septicemia, Blood cultures are the “gold standard” are based on the detection of viable microorganisms in the blood. The main aim of this is to identify the bacteria causing bloodstream infections and to determine and analysis their antibiotic sensitivity pattern in a tertiary center. In this study blood for culture was collected from 940 clinically suspected cases of blood stream infection from the hospital. Collected blood samples were processed in the bacteriology section at microbiology laboratory and standard laboratory methods were used to identified isolates and then antibiotic susceptibility test was performed using CLSI guidelines. Total 940 blood samples were cultured in which 139(14.8%) were found positive. Among isolates, the most predominant organism was Staphylococcus aureus (51.8%) followed by Escherichia coli (24.5%) and the least was Salmonella species (1.4%), Proteus species (1.4%) and Acinetobacter species (1.4%). Among Gram positive isolates, Penicillin and Erythromycin showed high degree of resistance. Imipenem was particularly susceptible among the isolated. Gentamicin and Amikacin showed high in vitro susceptibility for both Enterobacteriaceae and Nonfermenters. This study provides information on bacteriological profile of septicemic isolates. Therefore continuous monitoring of the susceptibility of organisms towards antibiotics is necessary to prevent and spread of drug resistance

Impact of rumors or misinformation on coronavirus disease (COVID-19) in social media

Introduction: The coronavirus disease 2019 (COVID-19) is a respiratory tract illness resulting from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, which has spread all over the globe, making it a major public health challenge across health systems. Simultaneously, numerous rumors, misinformation, and hoaxes appeared on several social media platforms regarding the etiology, outcomes, prevention, and cure of the disease1. The pressing issue is fake news spread more rapidly in social media than the ones from reliable sources and damages the authenticity balance of news ecosystem. Methodology: These articles contained diverse study methods (survey, content analysis, interview, literature review & others) and paradigm models (quantitative, qualitative) to identify the widespread misinformation and its impacts. Conclusion: Mainstream media platforms mostly contain fake news and rumors. The long-standing issue of misinformation regarding different socio-political issues is under constant discussion

In vivo studies of the angiogenic potential of Mandur Bhasma

Background: Mandur Bhasma is a herbo-mineral compound. It is prepared by Putapaka method. It is described as Raktasanjanan. In the current study, Mandur Bhasma was prepared with a standardized method w.s.r to Rasatarangini and an experimental study was done to observe the Angiogenic property of Mandur Bhasma. The current study will analyze angiogenic potential of Mandur Bhasma using chick CAM model. This research is intended to study the possible role of Mandur Bhasma on angiogenesis and establishing properties of Mandur Bhasma as an angiogenic by newer means. The experimental study inside the egg shell will be carried out on a membrane known as “chorioallantoic membrane”. Objectives:To Prepare Mandur Bhasma, Physicochemical and Analytical study of  Mandur Bhasma, To verify the angiogenic potential of Mandur bhasma using the chicken, horioallantoic membrane (CAM) model, To compare  Angeogenic potential of Mandur bhasma with standard drug progesterone. Methodology: Relevant classical literature regarding Mandur will be reviewed and the data will be collected. Mandur Shodhan with Gomutra and Mandur Maran with Triphala decoction will be done. Analytical Study like Organoleptic Test for Rasa, Gandha, Varna, Sparsha, Physicochemical Tests and other analytical test like ICP-AES /ICPMS, XRD structure of Bhasma, EDAX-NANO Particle Size will be done

To fabricate and evaluate the wound healing activity of herbal medicated plasters containing the extracts obtained from Tridax procumbens and Azadirachta indica

The present research work is to fabricate medicated plaster containing herbal extracts for wound healing properties. Till date no herbal medicated plaster have been prepared and evaluated using the extracts obtained from the plants Neem and Coatbutton. The objective of the present studyis to provide the herbal medicated plasters to the society. The majoradvantage of this medicated herbal plaster is its safety, efficacy, portability and efficiency. There is much more study such as Collection of plant, Extraction of plant materials and their antimicrobial test, Evaluation of qualitative and quantitative test, Estimation of total phenolic content, Antioxidant study, Antimicrobial study, Estimation of patch test, Antimicrobial studies of patch test. The plant extracts were quantitatively analysed for total flavonoids content, total phenolic content, and antioxidant activity. Methanolic extract of Azadirechta indica and Tridax procumbens has shown presence of high content of flavonoids and total phenolic content. Also the Azadirachtin content in the neem extracts was determined. All the six extracts AIME, AIPE, AIAE, TPME, TPPE and TPAE were subjected to antimicrobial activity.In patch test FII batch shows the excellent antimicrobial activity.FII patch was selected for fabrication of plasters which was then subjected to the pharmacological study

A Retrospective Study of Clinical and Laboratory Profile of Dengue Fever in Tertiary Care Hospital, Wardha, Maharashtra, India

Dengue disease is caused by dengue virus which is a vector born viral infection. The prevalence of dengue has increased dramatically from past few decades. The clinical symptoms vary from asymptomatic to severe hemorrhagic fever leads to high morbidity and mortality. Method- This retrospective study was carried out during January 2019 to December 2019. The laboratory test was done by using J Mitra & Co on Day 1 of their visit to the hospital which shows NS1, IgM and IgG reactivity towards dengue fever. The clinical profile information data was collected from patient records. Out of 640 samples 62.18% (398) was positive. The percentage of positive males and females were 60.78%, 64.23% respectively. The highest prevalence of dengue infection was observed more in age group between 31-40 years (81.69%) and least prevalence in age group 71-80 years (36.36%). Most of the patients were positive for NS1Ag121 (30.4%) followed by IgM positive 87 (21.9%) & 59 (14.8%) positive for both IgM and NS1 Ag The highest number of infections was seen during October (75.8%) post monsoon season. All patients had a fever, followed by head ache (82%) and body pains (66.4%), 64.84% stayed in the hospital less than 5 days. In tropical and sub-tropical regions dengue is still a leading public health problem especially during rainy and post monsoon seasons. Both NS1 Ag and IgM detection in early acute phase has potential diagnostic value. So, to reduce mortality there need to initiate community-based cohort studies to predict the pace of dengue spread based on clinical presentation and laboratory findings during epidemics in India. Aedesaegypti is a common vector for transmission of DENV, CHIKV and ZIKV, so there is a need to screen for these infections in endemic areas