PERANAN INTERLEUKIN-1 DALAM PEMBENTUKAN LESI ATEROSKLEROTIK PADA TIKUS PUT1H YANG DIBERI DIET ATEROGENIK = THE ROLE OF INTERLEUKIN-1 ON ATHEROSCLEROTIC LESION FORMING IN RAT ...

Atherosclerosis is a disease characterized by thickening of the artery wall and narrowing of its lumen. Although, people in the world have known that atherosclerosis is caused by high blood plasma concentration of LDL, and hardening and narrowing of arterial wall is due to various depositions including lipids, cholesterol and calcium, but little have known that atherosclerosis is an inflammatory process. Since interleukin-10 has known as one of mediator of inflammatory reaction that remains in atherogenic process, we assumed that ⢠another sitokin like interleukin-1 is also involved in that process. The purpose of this research was to study the interleukin-1 in atherogenesis in rat. Twenty male and female Sprague Dawley rats (10 each), 2 months of age, were used as experimental animals. Rats were weighed than allotted into 4 groups of 5 each : group I was male rats that fed basal diet, group II was male rats that fed atherogenic diet, group III was female rats that fed basal diet and group 1V was female rats that fed atherogenic diet. After 3 months on experi¬mental diet, all animals were weighed, the blood sample was collected into the EDTA tube for cholesterol and trigliceride levels, total white blood cell (WBC), monocyte and limpho¬cyte. All animals were then killed, the heart was taken out, saved into the bottle with 10 % of formaldehyde, for histopathologic and immunohistochemistry analyses. The result of the analyses of variance for factorial design showed that there were significant differences (