Development and Validation of Stability Indicating RP-HPLC-PDA Method for Determination of Acebrophylline and It's Application for Formulation Analysis and Dissolution Study

ABSTRACT A stability-indicating RP-HPLC-PDA method has been developed and subsequently validated for the determination of Acebrophylline in commercial capsules. The proposed HPLC method utilizes waters Cosmogel C18 column (150 mm x 4.6 mm, 5.0 μ particle size) and mobile phase consisting of methanol:acetate buffer (20:80 v/v) pH adjusted to 6 with glacial acetic acid at a flow rate of 0.85 mL/min and column was maintained at 50 0 C. Quantitation was achieved with UV detection at 274 nm based on peak area with linear calibration curves at concentration ranges 0.5-200 μg/mL for ACB (R 2 > 0.999). ACB and its drug products were exposed to acid, base and neutral hydrolysis, oxidation, heat and photolytic stress conditions. Under all these conditions, degraded products were well separated. The method was validated according to the ICH guidelines with respect to accuracy, precision, linearity, specificity, limits of detection, limits of quantitation and robustness. Method has been successively applied to assay of pharmaceutical formulation and dissolution study and no interference from the tablet excipients was observed. As the proposed method could effectively separate the drug from its degradation products, it can be employed as stability indicating method

Xanthogranulomatous salpingo-oophoritis: a case report and review of literature

Xanthogranulomatous inflammation of ovary is a rare disease that is characterized by presence of large number of lipid laden macrophages with an admixture of neutrophils, lymphocytes, plasma cells and multinucleated giant cells. It is misdiagnosed as ovarian tumour that leads to extensive surgery including hysterectomy. In this report we describe a case of Xanthogranulomatous salpingo-oophoritis along with review of literature

CASE REPORT: Papillary Adenoma of Kidney- An Incidental Autopsy Finding: Report of Two Cases

Background: Renal papillary adenoma is usually an incidental finding at autopsy with an incidence of 7% to 23%. The frequency of small papillary tumours of kidney increases with age to approximately 40% of the population over the age of 65. These tumours occur morefrequently in scarred kidneys, acquired renal cystic disease and in children with von Hippel-Lindau syndrome. Case history: In this report we describe renal papillary adenoma incidentally detected during autopsies of two elderly males. Gross examination of kidneyshowed two tiny subcapsular yellowish nodules in one case and single nodule with similar morphology in the other. Microscopic examination in both the cases showed a well circumscribed tumour composed of densely packed tubules and papillae lined by small cuboidal to columnar cells with rounded uniform nuclei. However there was no nuclear atypia, mitosis or necrosis

RNA Interference: A revolution in drug development

316-322RNA interference (RNAi) or gene silencing technology is a phenomenon by which double stranded RNAs elicit degradation of a target mRNA containing homologous sequence. It is essentially a new incarnation of well-established antisense principle. This technology enables the researchers to trigger post-transcriptional gene silencing in vivo. It is a robust method for lowering specific protein levels, compared to traditional techniques such as antisense, ribozymes or microinjection of function-blocking antibodies. RNAi offers a powerful tool for ascribing functions to genes while its application to in vivo models of disease opens up tremendous opportunities to develop a novel generation of oligonucleotide-based drugs, thus offering an enormous potential of being developed as a therapeutic modality

Pathologic lesions in children with acquired immunodeficiency syndrome an autopsy study of 11 cases from Mumbai, India

Background: Human immunodeficiency virus (HIV) infection in India has now been prevalent over three decades, and an increasing number of children are being affected with HIV. The spectrum of pathologic lesions in children with acquired immunodeficiency syndrome (AIDS) in India has not been well described. Materials and Methods: A review of systematically conducted autopsies of 11 (10 boys and 1 girl) children with AIDS is presented. Results: The mode of HIV transmission in 6 children was vertical; in one it was blood transfusion and in 4 children route was presumably vertical as these were children of orphanage. The clinical manifestations were failure to thrive; 9 children, persistent gastroenteritis; 8, recurrent fever; 5, bacterial infections; 5, hepatosplenomegaly; 5, candidiasis; 1, scabies; 1, skin rash; 2, tuberculous (TB) meningitis; 1 and paraplegia; in 1 child. The spectrum of pathologic lesions observed were precocious involution in thymus in 3 and dysinvolution in 2 cases. Infectious diseases comprised of TB; 4 cases, cytomegalovirus infection (CMV) 4; bacterial pneumonia and meningitis; 7, and esophageal candidiasis in 2 cases. Dual or multiple infections were observed in 9 (82%) cases; these comprised of two lesions in 2, three lesions in 2, four lesions in 4, and five lesions in 1 case. TB, bacterial pneumonia, meningitis, and CMV infection are the most frequent causes of death in children with AIDS. Vascular lesions showing features of arteriopathy were observed in 5 cases and brain in one case showed non-Hodgkin's lymphoma. Conclusions: This study provides a better insight into the spectrum of pathologic lesions in children with AIDS in India. TB and CMV infection has been found to be the most prevalent infection in our children

Structures of albanols a and b, two novel phenols from Morus alba bark

Structures for albanol A and B, isolated from Morus alba bark, have been proposed on the basis of X-ray analysis of albanol A methyl ether

Information Technology for Quality and Business Management System Optimization

1131-1137Quality management systems (QMS) such as, ISO series of international standards have emphasized on the fact that organizations should design their QMS to efficiently generate, process, store, and deliver appropriate document and data on quality and business performance to the top management, functional managers, and the entire human resource at each of the operational level within the organization. If QMS data were managed manually, quality and business management processes would slow down thereby reducing benefits to the organization. This paper, therefore, elaborates on appropriate application of information technology as a quicker method for analyzing QMS data to achieve better team/project management and optimizing quality and business processing systems, thereby ensuring efficiency and profitability of each functional unit of an organization so that overall performance and profitability of the organization can be monitored, measured, and controlled efficiently to run a smart business

Catalytic intermediates in carbonylation reactions. Part 2. Isolation, electronic, electrochemical characteristics and crystal structure of 2,6-diphenylpyridinium trans-dicarbonyl tetraiodorhodate(III)

Unusual halocarbonyl anions of Rh(III) having the formula [Rh(CO)<SUB>2</SUB>(I)<SUB>4</SUB>]<SUP>-</SUP> were isolated and characterized as 2,6-diphenylpyridinium and -isoquinolinium salts during the hydrocarbonylation of methyl acetate using RhCl<SUB>3</SUB>·3H<SUB>2</SUB>O as catalyst. The 2,6-diphenylpyridine salt [Rh(CO)<SUB>2</SUB>I<SUB>4</SUB>]<SUP>-</SUP>[C<SUB>17</SUB>H<SUB>14</SUB>N]<SUP>+</SUP> crystallizes in the space group P2<SUB>1</SUB>/n, with a=12.274(1), b=12.650(2), c=15.884(2) Å, β=93.31(1)°, V=2462.2(4) Å<SUP>3</SUP>, Z=4, final R=0.048. X-ray analysis shows a distorted octahedral environment around rhodium and the existence of trans- [Rh(CO)<SUB>2</SUB>I<SUB>4</SUB>]<SUP>-</SUP> species. The structural features indicate the influence of countercations on the electrochemical characteristics and stabilization of the trans isomer