A novel method for the development of latent fingerprints recovered from arson simulation

AbstractA diverse range of physical and chemical methods is available for the development of latent fingerprints. But fingerprints exposed to extreme conditions like fire or arson are generally perceived to have been damaged. Electromagnetic radiations, soot deposition and high temperatures are the forces generated in a fire, which may affect the fingerprints at the scene. Thus, the potential value of highly crucial evidence like fingerprints remains unutilized. This study was conducted to determine if fingerprints could be developed after being subjected to an arson/fire scene simulation. Fingerprints on nonporous surfaces were subjected to high temperatures, soot deposition and subsequently treated with water. A novel fluorescent and a pre-existing small particle reagent was investigated for the same. Zinc carbonate based fluorescent small particle reagent was capable of developing latent fingerprints exposed to a maximum temperature of 800°C

Optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) from Drosophila melanogaster brain tissue

Summary: Transposase-accessible chromatin by sequencing (ATAC-seq) has emerged as an advantageous technique to assess chromatin accessibility owing to the robustness of ''tagmentation'' process and a relatively faster library preparation. A comprehensive ATAC-seq protocol from Drosophila brain tissue is currently unavailable. Here, we have provided a detailed protocol of ATAC-seq assay from Drosophila brain tissue. Starting from dissection and transposition to amplification of libraries has been elaborated. Furthermore, a robust ATAC-seq analysis pipeline has been presented. The protocol can be easily adapted for other soft tissues. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics