The cell morphology of CEF inoculated NDV for each antibody group at different time (200×).

<p>CEF cells were seeded into 6-well plate, the antibody Mx-NA, Mx and NA was add into different well after the CEF cells grew into monolayer, then 100 TCID50 NDV was added into each well (in triplicates). The cell cultures were observed under microscope for cytopathic effect (CPE) at different time. MN group: Mx-NA antibody group; M group: Mx antibody group; N group: NA antibody group.</p

The plasmid profile of pVITRO₂-Mx-NA.

<p>Plasmid pVITRO₂-hygro-mcs was conducted, then Mx was inserted into MCS1<i>(Eco</i>R V and <i>Cla</i> I) and NA was insereted into the MCS2(<i>Eco</i>R I and <i>Xho</i> I restriction site).</p

HA of antibody group infected NDV at different time.

<p>After G418 selection for two weeks, the antibody incubated CEF cells were trypsinized and seeded into 24-well plates. After overnight cultivation, 100 TCID50 NDV was added to each well (in triplicates) and incubation was continued for 1 h at 37°C. After 24, 48 and 72 h infection, supernatants of the cell cultures were collected for NDV titration on CEF cells.</p

Transient expression of Mx and NA gene in transfected NIH 3T3 cells by IFA.

<p>A: The NIH 3T3 cells transfected with pVITRO2-Mx-NA (Mx antibody); B: The NIH 3T3 cells transfected with pVITRO2-Mx-NA (NA antibody). C: The NIH 3T3 cells transfected with pVITRO2; D : NIH 3T3 cells.</p

Primers used for real-time PCR.

1<p>TA, Annealing temperature.</p

Plasma contents (µmol/L) of neutral amino acids in Huanjiang mini-piglets with HBW¹ and LBW².

a-d<p>Values within a row without a common superscript letter differ (<i>P</i><0.05).</p>1<p>HBW, high birth weight;</p>2<p>LBW, low birth weight.</p

Antibodies and dilution used for Western blot analyses.

<p>Antibodies and dilution used for Western blot analyses.</p

Liver contents (%) of neutral amino acids in Huanjiang mini-piglets with HBW¹ and LBW ².

a-e<p>Values within a row without a common superscript letter differ (<i>P</i><0.05).</p>1<p>HBW, high birth weight;</p>2<p>LBW, low birth weight.</p

Protein abundances of jejunum B⁰AT1 (A) and ASCT2 (B) in sucking Huanjiang mini-piglets with HBW¹ and LBW² from days 0 to 21 of age.

<p>Samples were obtained from Huanjiang mini-pigs on Days 0, 7, 14 and 21 of age, respectively. The protein amounts of B0AT1 and ASCT2 were normalized using β-actin as an inner control. Values without a common letter on bars differ (<i>P</i><0.05). The <i>P</i> value showed the effects of age, BW, and age and BW interaction on protein expressiong of B0AT1 and ASCT2. Data are expressed as mean ± SD, n = 5. ¹HBW, high birth weight; ²LBW, low birth weight.</p