DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NIMBOLIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Objective: The present study was aimed to a simple, new, rapid and highly sensitive Reverse Phase - High Performance Liquid Chromatographic (RP-HPLC) method has been developed and an assay was validated for the quantitative estimation of nimbolide in solid dosage form.Methods: The chromatographic separation was achieved on an Agilent 1200 series HPLC system C18 (250 mm x 4.6 mm x 2.5 μ) column packing by using isocratic mobile phase consisting of acetonitrile: water (90:10 v/v), flow rate was adjusted to 1.0 ml/min at a fixed wave length of 207 nm.Results: The nimbolide was eluted at 2.880 ± 0.05 min and established a dynamic range of linearity over the concentration range of 3.125-200 ppm/ml (r2 = 0.9997 ± 0.005). The lower limit of detection and quantification was 0.007 ppm/ml and 0.021 ppm/ml respectively. The method was validated as per ICH guidelines. The accuracy of the method was determined by the recovery studies and the mean recovery was obtained 98.27%. Moreover the method was shown good reproducibility and recovery with percent relative standard deviation less than 2%.Conclusion: Rapid, efficient and sensitive RP-HPLC method was developed for the estimation of nimbolide from the perspective of reducing the cost of analysis and time and thus by saving laboratory resources.Â

IN VITRO ANTHELMINTIC ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF OLDENLANDIA UMBELLATA

Objective: The present study was carried out to evaluate the in vitro anthelmintic activity of aqueous and methanolic extracts of Oldenlandia umbelata against Pheretima posthuma. Methods: Three different concentrations, each of crude alcoholic and aqueous extract (10, 50,100 mg/ml in distilled water) were prepared and six worms (same type) were placed in it. Observations were made for the time taken to cause paralysis and death of the individual worms. Mean time for the paralysis (P) in min was noted when no movement of any sort could be observed,except when the worm was shaken vigorously; time of death (D) in min was recorded after ascertaining the worms neither moved when shaken vigorously nor when dipped in warm water (50°C). Piperazine citrate (10mg/ml) was included as reference compounds. Results: The extracts of Oldenlandia umbelata not only demonstrated paralysis, but also caused death of worms, especially at higher concentration of 100 mg/ml in shorter time as compared to reference drug Piperazine citrate. Conclusion: In present statement methanolic and aqueous extracts of plant of Oldenlandia umbelata was investigated for their anthelmintic activity against Pheretima posthuma. Various concentrations were used in the bioassay, which involved paralysis and death time of the worms. Both the extracts showed significant anthelmintic activity

Development and Validation of a Rapid RP HPLC Method for the Determination of Cinitapride Hydrogen Tartarate in Solid Oral Dosage Forms

In the present study a simple, sensitive rapid and accurate HPLC method with UV detection for the analysis of cinitapride hydrogen tartarate was developed and validated in solid dosage forms. The method utilized gradient elution technique with C18 column (150×4.6 mm I.D, 5 μm particle size) with mobile phase consisting of 0.1% formic acid in water and acetonitrile The detection wavelength was at 268 nm, with flow rate of 0.5 mL/min and injection volume of 10 μL for separation of cinitapride in bulk drugs and pharmaceutical formulations. The gradient elution was developed for better and optimized results. The developed method was validated for precision which includes system precision and method precision, accuracy and linearity studies in the concentration range of 5-100 μg/mL with correlation coefficient of 0.9987. The accuracy (recovery) was between 97.32 and 100.82%. The proposed method is simple, fast, accurate, precise and reproducible, hence can be applied for routine quality control analysis of cinitapride in pure and pharmaceutical dosage forms

DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR QUANTITATIVE ESTIMATION OF VASICINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

  Objective: The aim was to develop and validate a new, rapid, and highly sensitive high performance liquid chromatography (HPLC) method for the quantitative estimation of vasicine in bulk and pharmaceutical dosage form, according to International Conference on Harmonization (ICH) guideline.Methods: The chromatographic separation was achieved on an agilent 1200 series HPLC system phenyl (250 mm×4.6 mm×5 μm) column packing, using a mobile phase consisting of hexane sulphonic acid-acetonitrile-acetic acid (60:20:1; v/v/v) in isocratic mode. The flow rate was set at 1.0 ml/minute, and ultraviolet detection was monitored at 300 nm.Results: The method was linear in the concentration range of 3.125-200 ppm/ml with a correlation coefficient of 0.999. The retention time for vasicine was found to be 5.30±0.05 minutes. The main recoveries obtained in the range of 90.476-107.1%, shows that the developed method was accurate and precise (<2% relative standard deviation). The lower limit of detection and limit of quantification were 3.0208 and 9.1541 μg/ml, respectively.Conclusion: The proposed method met the general requirements with an acceptable performance for validation. This selective method is found to be reliable, accurate, and effectively used for the vasicine. The result showed that the method is achieved a good performance with simple, rapid and accurate characteristics for quantification of vasicine in pharmaceutical preparations. The proposed method can be employed for the routine analysis of the quality of herbal extracts and in formulations.Keywords: High-performance liquid chromatography, Vasicine, International conference on harmonisation guidelines, Validation, Phenyl column.INTRODUCTIO

ANTIBACTERIAL POTENTIAL OF METHANOLIC EXTRACT OF GYROCARPUS ASIATICUS WILLD

Objective: The present study was designed to evaluate the antibacterial activity of the methanolic extract of Gyrocarpus asiaticus Willd. Methods: The Gyrocarpus asiaticus Willd extract under study has been screened for the antibacterial activity against with Staphylococcus aureus, Escherichia coli, Salmonella typhi, Salmonella paratyphi, pseudomonas mirebelis, Pseudomonas auroginosa and Klebsiella pneumoni human pathogenic bacteria by disc diffusion method. Results: Methanolic extract showed satisfactory results against almost all the organisms, among them against E.coli it showed highest zone of inhibition 18.5mm (0.4g/ml) with the minimum inhibitatory concentration (MIC) value of 0.039 mg/ml and minimum bactericidal concentration (MBC) of 0.16mg/ml. Other Gram negative pathogens like P. mirabelis, P. auroginosa shows good zone of  inhibition 13.9 mm and 17 mm (0.4g/ml) with  less minimum inhibitatory concentration of 0.039 mg/ml and MBC values of  0.16 mg/ml for the  above mention bacteria. But klebsiella sp was found to be resistant with no MIC, no MBC value and with a very less zone of inhibition of 7.5mm (0.4g/ml) when compared with the standards. Conclusion: From the results, it is evident that Gyrocarpus asiaticus Willd is recommended as a plant of an antibacterial agent. Keywords: Gyrocarpus asiaticus Willd, methanolic extract, antibacterial activity, MIC, MBC

Studies of anticancer and antipyretic activity of Bidens pilosa whole plant

Screening of different extracts and fractions from the plant Bidens pilosa Linn. var. (Asteraceae) has been conducted using the in - vitro comet assay for anticancer and the antipyretic action, which was done with in - vivo models. The extract from whole plant was extracted with n - hexane, chloroform and methanol extract (E1 – E3). The extracts were fractioned by column chromatography method and fractioned with ethyl acetate, acetone and water (F1 – F3). All the extracts and fractions were tested for anticancer and antipyretic activity. Among extracts E1 shows remarkable anticancer activity and E3 bears maximum antipyretic activity. In the antipyretic activity, paracetamol was used as the standard test drug. The most promising material (LC50 < 1500 µg / ml) was F1 ethyl acetate fractions of methanolic extract and methanolic crude extract of whole plants. However, little correlation was observed in the degree of antipyretic activity between the test drug and standard drug. In conclusion, the extract obtained from the whole plant of Bidens pilosa showed a significant cytotoxic effect to methanolic extract against Hela cells by in vitro method and showed a comparable antipyretic activity effect to paracetamol in rabbit pyrogen test