PERBANDINGAN EFEK PEMBERIAN TERAPI NEUROMUSCULAR ELECTRICAL STIMULATION INTENSITAS RENDAH DENGAN INTENSITAS TINGGI TERHADAP PENINGKATAN PEMBENTUKAN FIBROBLAS DAN KAPILER PADA MODEL CEDERA PARSIAL TENDON ACHILLES NEW ZEALAND WHITE RABBIT

Latar Belakang: Neuromuscular Electrical Stimulation (NMES) adalah salah satu modalitas yang bisa menjadi pilihan terapi pada cedera tendon. Namun sayangnya, belum ada studi yang melakukan secara klinis pada manusia karena masih terbatasnya studi yang dilakukan pada hewan coba. Salah satunya, mengenai perbedaan besar arus listrik. Dari hasil studi yang sudah ada, belum didapatkan konsensus protokol mengenai besar aliran arus listrik yang diberikan. Tujuan: Membandingkan efek terapi neuromuscular electrical stimulation (NMES) antara kelompok kontrol tanpa stimulasi dengan intensitas rendah dan intensitas tinggi terhadap jumlah fibroblas dan jumlah kapiler pada proses penyembuhan cedera parsial tendon Achilles New Zealand White Rabbit. Metode: 30 New Zealand White Rabbit jantan terbagi dalam 3 kelompok (kontrol, intensitas rendah dan inensitas tinggi). Setelah dilakukan pembuatan cedera parsial tendon Achilles kanan, 24 jam kemudian mulai diberikan stimulasi NMES intensitas rendah 1mA dan intensitas tinggi 80mA. Akhir minggu kedua dan keempat dilakukan terminasi dan dibuat preparat histopatologi jaringan tendon untuk dilakukan penghitungan jumlah fibroblas dan jumlah kapiler. Hasil: Terdapat perbedaan bermakna (p<0,05) rerata jumlah fibroblas antara kelompok kontrol dan intensitas rendah, kelompok kontrol dan intensitas tinggi, serta kelompok intensitas rendah dan intensitas tinggi pada minggu kedua dan keempat. Terdapat perbedaan bermakna (p<0,05) rerata jumlah kapiler antara kelompok kontrol dan intensitas rendah, kelompok kontrol dan intensitas tinggi, serta kelompok intensitas rendah dan intensitas tinggi pada minggu kedua. Tidak didapatkan perbedaan bermakna (p>0,05) rerata jumlah kapiler antara kelompok kontrol, intensitas rendah dan intensitas tinggi pada minggu keempat. Kesimpulan: Terapi neuromuscular electrical stimulation baik intensitas rendah atau intensitas tinggi dapat meningkatkan jumlah fibroblas dan jaringan kapiler jaringan tendon, namun tidak dapat meningkatkan jumlah kapiler pada minggu keempat pasca intervensi pada proses penyembuhan cedera parsial tendon Achilles New Zealand White Rabbit

Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells as an Alternative Cell Source for Cell-based Osteoarthritis Treatment: A Systematic Review on Preclinical and Clinical Evidence

ABSTRACT Introduction: There is still no definitive treatment to either inhibit the degradation process or restore the damaged cartilage in osteoarthritis (OA). Various cell sources have been studied and in vitro studies showed that infrapatellar fat pad-derived mesenchymal stem cells (IFPDMSCs) exhibit higher chondrogenic potential than other adipose-derived cells. Still, very few in vivo studies on IFPDMSCs for cartilage healing in OA have been reported. Aims: This systematic review will analyze the therapeutic potential of IFPDMSCs for cartilage healing in osteoarthritis from preclinical and clinical studies. Design, Methods, and Data Source: Using the PubMed, EMBASE, and Cochrane Library database up to November 30, 2020, a systematic review according to PRISMA reporting guideline was conducted on IFPDMSCs application to treat osteoarthritis in vivo studies. Inclusion criteria were in vivo preclinical and clinical studies from January 2010 to November 2020 involving the OA model or cases using IFPDMSCs to promote healing. Results: In vivo studies are scarce. Only four studies are included: two animals and two clinical studies. All included studies demonstrate favourable results of IFPDMSCs in osteoarthritis, but there is heterogeneity in outcome measurement among all studies. Conclusion: The in vitro and currently limited in vivo studies showed that infrapatellar fat pad-derived mesenchymal stem cells offer an alternative cell source with promising chondrogenic healing potential. Impact: More preclinical and clinical in vivo studies should be encouraged to explore and support the efficacy of IFPDMSCs in cell-based OA treatment to prove the promising result as those of the in vitro studies

Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells as an Alternative Cell Source for Cell-based Osteoarthritis Treatment: A Systematic Review on Preclinical and Clinical Evidence

ABSTRACT Introduction: There is still no definitive treatment to either inhibit the degradation process or restore the damaged cartilage in osteoarthritis (OA). Various cell sources have been studied and in vitro studies showed that infrapatellar fat pad-derived mesenchymal stem cells (IFPDMSCs) exhibit higher chondrogenic potential than other adipose-de�rived cells. Still, very few in vivo studies on IFPDMSCs for cartilage healing in OA have been reported. Aims: This systematic review will analyze the therapeutic potential of IFPDMSCs for cartilage healing in osteoarthritis from pre�clinical and clinical studies. Design, Methods, and Data Source: Using the PubMed, EMBASE, and Cochrane Library database up to November 30, 2020, a systematic review according to PRISMA reporting guideline was conducted on IFPDMSCs application to treat osteoarthritis in vivo studies. Inclusion criteria were in vivo preclinical and clinical studies from January 2010 to November 2020 involving the OA model or cases using IFPDMSCs to promote healing. Results: In vivo studies are scarce. Only four studies are included: two animals and two clinical studies. All included studies demonstrate favourable results of IFPDMSCs in osteoarthritis, but there is heterogeneity in outcome measure�ment among all studies. Conclusion: The in vitro and currently limited in vivo studies showed that infrapatellar fat pad-derived mesenchymal stem cells offer an alternative cell source with promising chondrogenic healing potential. Impact: More preclinical and clinical in vivo studies should be encouraged to explore and support th