Carbon Fibers in Biomedical Applications

Three-dimensional growth of fibroblasts on carbon fibre mesh and assessment of biocompatibilty by in vitro and in vivo examination was done. Suitable size carbon fiber mesh after sterilization, placed in six well cell culture plate. The mesh was co-cultured with p-MEF cells. At different time intervals the viability and proliferation of the p-MEF cells was evaluated. The primary objective of this study was biological evaluation of carbon fibre mesh which can be used for creation of three-dimensional scaffolds for tissue engineering. Among the possible forms of implants, fibrous matrices are highly promising for the tissue regeneration by acting as a cell-supporting scaffold. Results of in vitro observations of the morphology p-MEF cells seeded on the surface of carbon fibre mesh shows adhesions and attachment of fibroblasts cells to carbon fibres on day 3 post seeding. They attached firmly and were uniformly spread along the fibres on day 5 postseeding and mostly spindle-shaped and cover almost all their surface on day 7 postseeding and such a spreading of cells indicates good adhesions and biocompatibility of carbon fibres. In vivo examination of retrieved sample on day 30 post implantation shows that carbon fibre mesh was covered by dense thick fibrous connective tissue

Usporedba lidokaina i kombinacije lidokaina i ketamina primijenjenih za distalnu intravensku regionalnu anesteziju (DIVRA) u goveda

The hoof diseases of cattle can be managed surgically under intravenous regional anesthesia (IVRA). For routine induction of IVRA, a tourniquet is placed circumferentially at the metacarpus/metatarsus. In the present study, hoof diseases of cattle were corrected using a modified IVRA technique. The cattle with hoof ailments were randomly divided into two groups and a tourniquet was placed just distal to the dew claws instead of at the metacarpus/metatarsus in order to decrease the dose of anesthetic. In group I lidocaine (2mg/kg) and in group II a mixture of lidocaine and ketamine (2mg/kg+1.5mg/kg) was injected into the axial digital vein to induce distal intravenous regional anesthesia (DIVRA). The heart rate, respiration rate, systolic and diastolic pressure were unaffected in both groups. Oxygen saturation was significantly (P<0.05) lower between 5 and 60 minutes in group I and between 15 and 40 minutes in group II animals. The sensory and motor block onset time was shorter, and the sensory and motor block recovery time was longer in group II animals as compared to group I animals. It was concluded that the DIVRA technique using lidocaine alone and lidocaine admixed with ketamine are suitable for hoof examination and surgery.Bolesti papaka u goveda mogu se kirurški liječiti pod intravenskom regionalnom anestezijom (IVRA). Za rutinsko uvođenje u IVRA-u postavlja se kružno čvrsti zavoj na metakarpus/metatarzus. U ovom su istraživanju bolesti papaka u goveda liječene modificiranom IVRA metodom. Istražene životinje nasumično su podijeljene u dvije skupine a zavoj kojim se samnjuje doza anestetika postavljen je, umjesto na metakarpus/metatarzus, distalno od rudimentiranih papaka. U skupini I primijenjen je lidokain (2 mg/kg), a u skupini II kombinacija lidokaina i ketamina (2 mg/kg + 1,5 mg/kg). Za uvođenje u distalnu intravensku regionalnu anesteziju (DIVRA) anestetici su aplicirani u aksijalnu digitalnu venu. Srčana frekvencija, frekvencija disanja, sistolički i dijastolički tlak u obje su skupine bili nepromijenjeni. Zasićenost kisikom bila je znakovito niža (P<0,05) između 5. i 60. minute u skupini I te između 15. i 40. minute u skupini II. Vrijeme pojave senzornih i motoričkih blokova bilo je kraće, a vrijeme oporavka tih blokova dulje u životinja u skupini II u usporedbi sa skupinom I. Zaključeno je da je DIVRA, uz upotrebu i samog lidokaina i lidokaina u kombinaciji s ketaminom, prikladna metoda za pregled i obavljanje kiruških zahvata na papcima goveda