ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES SYNTHESIZED USING ULVA RETICULATA

Production and application of silver nanoparticles (AgNPs) is an emerging field of research. The present study was demonstrated with rapid synthesis of AgNPs using marine macroalgae-Ulva reticulata. The observation of prominent colour change at 121ºC within 10 mins indicates the formation of AgNPs. The synthesized AgNPs were characterized by UV-Vis spectrum, Energy Dispersive Spetrum (EDS), Fourrier Transform Infra Red spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). The aim of the study is to evaluate the antibacterial and antifungal activity of synthesized AgNPs. Antibacterial activity was tested against Gram positive Staphylococcus aureus, and Gram negative Escherichia coli, Pseudomonas aeruginosa, Bacillus sp., Klebsiella pneumoniae. Antifungal activity was tested against Candida albicans, Candida parapsilosis and Aspergillus niger. AgNPs were fairly toxic to Bacillus sp.and Staphylococcus aureus with the inhibition zone of 26 and 25mm. AgNPs revealed higher antifungal activity against Candida albicans, Candida parapsilosis and Aspergillus niger with inhibition zone of 36, 30 and 30mm. Key words:Silver nanoparticles, Marine maro algae-Ulva reticulata, antibacterial, antifungal activity

<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Production of biogenic Silver nanoparticles using <i style="mso-bidi-font-style: normal">Sargassum longifolium</i> and its applications</span>

125-130Present study consists a simple and eco-friendly biosynthesis of silver nanoparticles using Sargassum longifolium aqueous extract as reducing agent. Aqueous silver ions when exposed to seaweed extract were reduced and results in silver nanoparticles. Characterization of these silver nanoparticles were done using UV-Vis spectroscopy, Fourier Transform Infra red (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM) and Energy Dispersive analysis X-ray (EDS) Spectrum. Cytotoxicity assay was done against Hep 2 cell line. The cytotoxicity analysis of the sample shows a direct dose-response relationship; cytotoxicity increased at higher concentrations

<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Green synthesis and cytotoxicity of silver nanoparticles from extracts of the marine macroalgae <i style="mso-bidi-font-style:normal">Gracilaria corticata</i></span>

276-281<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-GB">The present study reports the synthesis of silver nanoparticles (AgNPs) using aqueous extract of a seaweed, Gracilaria corticata. Extremely stable AgNPs were characterized by UV-Vis spectrophotometer, FTIR, XRD, TEM and EDS analyses. The nanoparticles exhibited maximum absorbance at 424 nm in the UV spectrum. The presence of proteins was identified by FTIR. TEM micrograph revealed the formation of polydispersed and spherical shaped nanoparticles with the size range of 10-50 nm and the presence of elemental silver were confirmed by EDS analysis. These nanoparticles showed cytotoxic activity against MCF7 cells.</span