Evaluating detectability of freshwater fish assemblages in tropical streams:is hand-seining sufficient?

Unprecedented threats to natural ecosystems mean that accurate quantification of biodiversity is a priority, particularly in the tropics which are underrepresented in monitoring schemes. Data from a freshwater fish assemblage in Trinidad were used to evaluate the effectiveness of hand-seining as a survey method in tropical streams. We uncovered large differences in species detectability when hand-seining was used alone, in comparison with when hand-seining and electrofishing were used together. The addition of electrofishing increased the number of individuals caught threefold, and increased the biomass fivefold. Some species were never detected using hand-seining, resulting in significant underestimates of species richness; rarefaction curves suggest that even when hand-seining effort increases, species richness is still underestimated. Diversity indices (Shannon and Simpson index) reveal that diversity was also significantly lower for hand-seined samples. Furthermore, the results of multivariate analyses investigating assemblage structure also differed significantly depending on whether they were based on hand-seined data alone, or a combination of hand-seining and electrofishing. Despite the extra equipment and maintenance required, these findings underline the value of including electrofishing when sampling tropical freshwater streams