A COMPARATIVE STUDY ON STUDENTS’ SCORES THROUGH COLLABORATIVE LEARNING AND NON-COLLABORATIVE LEARNING AT SAINT JOSEPH BANGNA SCHOOL, THAILAND

In 1999, Thailand triggered some drastic changes and reforms by implementing the National Education Act (NEA). Accordingly, students were encouraged to become critical thinkers, and to acquire information technology knowledge based on the student-centred model. These reforms have socio-constructivist roots, promoting the use of collaborative learning, and the use of information technology. Almost fifteen years after the implementation of the NEA, the purpose of the current study was to examine if collaborative learning, for the computer science subject, would be beneficial for the grade seven Thai students of the English Program at Saint Joseph Bangna School or, as some research stated, it would be very hard to implement because of cultural hindrances. This study had three objectives. The first objective was to compare the difference among the pre-tests and post-tests scores of the experimental group who studied through collaborative learning. The second objective was to compare the difference among the pre-tests and post-tests scores of the control group who studied through non-collaborative learning. The last objective was to compare the difference among the post-tests scores of the experimental group who studied through collaborative learning with the control group who studied through non-collaborative learning. The pre-test and the post-test were the same, and consisted of 25 multiple choice questions based on a Microsoft Office Specialist certification test for the Microsoft Excel software. In conclusion, this study suggests that collaborative learning delivers better outcomes for Thai students for the computer science subject

XTcf-3 Transcription Factor Mediates β-Catenin-Induced Axis Formation in Xenopus Embryos

AbstractXTcf-3 is a maternally expressed Xenopus homolog of the mammalian HMG box factors Tcf-1 and Lef-1. The N-terminus of XTcf-3 binds to β-catenin. Microinjection of XTcf-3 mRNA in embryos results in nuclear translocation of β-catenin. The β-catenin–XTcf-3 complex activates transcription in a transient reporter gene assay, while XTcf-3 by itself is silent. N-terminal deletion of XTcf-3 (ΔN) abrogates the interaction with β-catenin, as well as the consequent transcription activation. This dominant-negative ΔN mutant suppresses the induction of axis duplication by microinjected β-catenin. It also suppresses endogenous axis specification upon injection into the dorsal blastomeres of a 4-cell-stage embryo. We propose that signaling by β-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes

L'obligation de motivation formelle dans le cadre du licenciement à la lumière des droits fondamentaux : analyse et comparaison de deux régimes

Le 12 février 2014, les partenaires sociaux se mettaient d'accord et la Convention collective de travail n° 109 concernant la motivation du licenciement voyait le jour. Cette dernière a pour but de rendre obligatoire la motivation du congé lors de la demande du travailleur dans le secteur privé. Grâce à cette Convention, la Belgique se rapproche un peu plus des garanties et exigences internationales. A côté de cela, le secteur public reste pour l'instant soumis à la loi du 29 juillet 1991 relative à la motivation formelle des actes administratifs, en attendant la création d'un régime analogue à celui de la Convention n° 109. La démarche entreprise vise à effectuer une comparaison de ces deux régimes à la lumière des garanties fondamentales qui protègent les travailleurs, c'est-à-dire qui offrent un droit à la connaissance des motifs liés au licenciement. Le but est de déterminer lequel des deux secteurs alloue la meilleure protection à ses travailleurs. Il est intéressant alors d'essayer de déterminer si et comment on pourrait améliorer l'un ou l'autre de ces régimes.Master [120] en droit, Université catholique de Louvain, 2015La diffusion de ce mémoire n'est pas autorisée par l'institutio

Maternal XTcf1 and XTcf4 have distinct roles in regulating Wnt target genes

AbstractWnt signaling pathways have essential roles in developing embryos and adult tissue, and alterations in their function are implicated in many disease processes including cancers. The major nuclear transducers of Wnt signals are the Tcf/LEF family of transcription factors, which have binding sites for both the transcriptional co-repressor groucho, and the co-activator β-catenin. The early Xenopus embryo expresses three maternally inherited Tcf/LEF mRNAs, and their relative roles in regulating the expression of Wnt target genes are not understood. We have addressed this by using antisense oligonucleotides to deplete maternal XTcf1 and XTcf4 mRNAs in oocytes. We find that XTcf1 represses expression of Wnt target genes ventrally and laterally, and activates their expression dorsally. Double depletions of XTcf1 and XTcf3 suggest that they act cooperatively to repress Wnt target genes ventrally. In contrast, XTcf4 has no repressive role but is required to activate expression of Xnr3 and chordin in organizer cells at the gastrula stage. This work provides evidence for distinct roles for XTcfs in regulating Wnt target gene expression

ASC1 complex related conditions: Two novel paediatric patients with TRIP4 pathogenic variants and review of literature

Pathogenic variants in the genes encoding for the ASC1 complex were recently reported in patients with congenital fractures, joint contractures, neonatal hypotonia and respiratory distress. Here we report two male children with biallelic TRIP4 pathogenic loss of function variants. The first child presented with foetal bradykinesia, neonatal respiratory distress, central and peripheral hypotonia, constipation, hyperlaxity, left uretero-hydronephrosis and post-obstructive kidney dysplasia. The second had severe central and peripheral neonatal hypotonia, feeding difficulties, kyphosis, developmental delay and hyperlaxity. Detailed review of all reported cases with ASCC1 (12 patients) and TRIP4 (18 patients) variants highlights striking genotype-phenotype correlations. This is the fourth report of patients with TRIP4 variants and the first description of post-obstructive kidney dysplasia in this condition

PR72, a novel regulator of Wnt signaling required for Naked cuticle function

The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required for early segmentation. In addition, Naked cuticle is proposed to function as a switch, acting to restrict classical Wnt signaling and to activate a second Wnt signaling pathway that controls planar cell polarity during gastrulation movements in vertebrates. Little is known about the biochemical function of Naked cuticle or its regulation. Here we report that PR72, a Protein Phosphatase type 2A regulatory subunit of unknown function, interacts both physically and functionally with Naked cuticle. We show that PR72, like Naked cuticle, acts as a negative regulator of the classical Wnt signaling cascade, establishing PR72 as a novel regulator of the Wnt signaling pathway. Our data provide evidence that the inhibitory effect of Naked cuticle on Wnt signaling depends on the presence of PR72, both in mammalian cell culture and in Xenopus embryos. Moreover, PR72 is required during early embryonic development to regulate cell morphogenetic movements during body axis formation

Novel defects in collagen XII and VI expand the mixed myopathy/Ehlers-Danlos syndrome spectrum and lead to variant-specific alterations in the extracellular matrix

Purpose To date, heterozygous or homozygous COL12A1 variants have been reported in 13 patients presenting with a clinical phenotype overlapping with collagen VI-related myopathies and Ehlers-Danlos syndrome (EDS). The small number of reported patients limits thorough investigation of this newly identified syndrome, currently coined as myopathic EDS. Methods DNA from 78 genetically unresolved patients fulfilling the clinical criteria for myopathic EDS was sequenced using a next-generation panel of COL12A1, COL6A1, COL6A2, and COL6A3. Results Among this cohort, we identified four pathogenic heterozygous in-frame exon skipping ( increment ) defects in COL12A1, clustering to the thrombospondin N-terminal region and the adjacent collagenous domain (Delta 52, Delta 53, Delta 54, and Delta 56 respectively), one heterozygous COL12A1 arginine-to-cysteine substitution of unclear significance (p.(Arg1863Cys)), and compound heterozygous pathogenic COL6A1 variants (c.[98-6G>A];[301C>T]) in one proband. Variant-specific intracellular accumulation of collagen XII chains, extracellular overmodification of the long isoform and near-absence of the short isoform of collagen XII, and extracellular decrease of decorin and tenascin-X were observed for the COL12A1 variants. In contrast, the COL6A1 variants abolished collagen VI and V deposition and increased tenascin-X levels. Conclusion Our data further support the significant clinical overlap between myopathic EDS and collagen VI-related myopathies, and emphasize the variant-specific consequences of collagen XII defects

Monoclonal autoantibodies recognizing histone variants

Balb/c mice were immunized with affinity purified Ro(SS-A) from human origin in order to allow the preparation of monoclonal anti-Ro(SS-A) antibodies. After fusion of mouse myeloma cells (line Sp2/0 A914) with spleen cells from one of these mice, anti-Ro(SS-A) monoclonals were not obtained, but, instead, two IgM producing hybridomas reactive with histone H1 and one with histone H2B. The specificity of the anti-H1 monoclonals was investigated by means of immunoblotting of very lysine-rich histone variants from mouse which were separated by two-dimensional gelelectrophoresis. One of them (CLB-ANA 105) has H1 o specificity with respect to the histone variants of mouse and man, but recognizes H5 as well as H5 from Xenopus laevis Another monoclonal (CLB-ANA 108) reacts with the variant H1 c from mouse, exclusively. From the way these monoclonals were produced, we postulate that they were not the result of immunization, but comprise specifities of naturally occurring autoantibodies. © 1988 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted