The Introduction Into Tobacco Plants of Genes Which Encode Some of the Natural Components of the Humoral Immune Response of Hyalophora Cecropia.

A number of plant pathogenic bacteria has been shown to be highly sensitive to SB-37, a derivative of cecropin B (one of the three cecropins from the giant silk moth Hyalophora cecropia). The lytic activity exhibited by SB-37 has been shown to increase up to twenty-fold in the presence of chicken egg white lysozyme. The cDNAs genes encoding SB-37, attacin E (another component of the humoral response of Hyalophora cecropia), and chicken lysozyme have been introduced into tobacco (var Xanthi) and potato (a new cultivar 86007) plants by Agrobacterium-mediated transformation using co-integrate and binary vectors. The genes were placed under the control of different promotors: CaMV35S, Double-CaMV35S and the proteinase inhibitor II promoter from potato. The transformation of at least five independent lines for each construct has been confirmed by Southern blot and segregation analysis. Protein analysis demonstrated that the chicken egg white lysozyme was expressed in tobacco plants. Western analysis of Attacin E-transformed plants, failed to show expression of the gene at the protein level. Protein blot analysis of SB-37 transgenic lines gave unconclusive results indicating at least very low level of expression. When these plants were inoculated with P. solanacearum, at least two independent lines, showed symptoms earlier than control plants indicating some biological effect. The significance of this result is discussed in detail. Also, the characterization of different de novo designed peptides is described

Forest biology project advisory committee meeting

"October 15-16, 1997."Mass clonal propagation of improved conifers - elementals analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Combined effects of activated carbon and pH on ionic composition and 2,4-D availability in a tissue culture medium / S.C. Van Winkle, G.S. Pullman ; F010: Mass clonal propagation of softwoods: functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Analysis of stage specific bands from differential display of loblolly pine somatic embryos / John Cairney and Barbara Johns ; Analysis of somatic embryo development using differential display I: advancements in techniques / Nanfei Xu, Gerald Pullman and John Cairney ; Analysis of somatic embryo development using differential display II: cloning, sequencing and characterization of genes differentially expressed at different stages of somatic embryos / Nanfei Xu ... [et al.] ; Analysis of embryo development using differential display III: cloning, sequencing and characterization of genes differentially expressed at different stages of zygotic embryos / Nanfei Xu ... [et al.] ; Isolation and characterization of loblolly pine embryo specific vicilin-like storage protein gene; use of this gene as a molecular marker for somatic embryo sorting / Ranjan Perera ... [et al.] ; Applying expertise in flowering research to embryogenesis I: expression of floral homeotic genes in pine embryos / Lin Ge ... [et al.] ; Applying expertise in flowering research to embryogenesis II: isolation and characterization of flower specific (homeotic) genes from loblolly pine, and expression of these genes in vegetative tissues / Ranjan Perera ... [et al.] ; F011: Mass clonal propagation of hardwoods: establishing and improving the zinnia elegans tracheary element differentiation system at IPST / Gary Peter, Karen Floyd, Chris Ricker ; Gene regulation in woody plants I: promoter and 3'UTR effects: regulation of expression of a stress-related gene: promoter and 3'UTR analysis-an update / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants II: 5'UTR effects: regulation of expression and gene structure of a down-regulated gene during drought stress / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants III: identification of a plant enzyme which activates a regulatory peptide / Luis Destefano ... [et al.] -- Slide Material

Annual program review, Forest Biology, March 25-26, 1997

"March 25-26, 1997."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: long-term storage of loblolly pine somatic embryo cultures in liquid nitrogen / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Mass clonal propagation of improved conifers: progress in protocol development and targets / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman, Mike Buchanan, Yolanda Powell ; Mass clonal propagation of improved conifers: antibody markets, straining procedures and image analysis for characterizing pinus taeda embryogenesis / Gary Peter, Levi Barclay ; Mass clonal propagation of improved conifers: construction of cDNA libraries from zygotic embryos of pinus taeda / Gary Peter / Gerald Pullman ; Differential display as a tool to understand and improve somatic embryogenesis / John Cairney ; Monitoring gene expression in somatic embryos of an elite genotype of loblolly pine / John Cairney and Barbara Johns ; Differential display to compare gene expression in loblolly pine zygotic and somatic embryos / Vincent Ciavatta, Jerry Pullman and John Cairney ; Analysis of somatic embryo development using differential display: rapid identification, cloning and sequencing of marker bands by an improved method / Nanfei Xu, Jerry Pullman, and John Cairney ; Preliminary comparison of zygotic and somatic embryo development using differential display / Nanfei Xu, Jerry Pullman and John Cairney ; Gene expression during loblolly pine embryogenesis: drought-inducible genes also expressed in pine cell suspension culture / Ranjan Perera and John Cairney ; Mass clonal propagation of genetically improved and engineered hardwoods: project F011 / Gerald Pullman ... [et al.] ; Agrobacterium tumefaciens-mediated transformation of populus deltoides leaf sections / Ronald J. Dinus ... [et al.] ; Regulation of expression of a stress-related gene: promoter and 3' UTR analysis / Luis Destefano and John Cairney ; Identification of a plant enzyme which activates a regulatory peptide / John Cairney ... [et al.] ; Three year research plan for mass clonal propagation of genetically improved and engineered hardwoods 1997-2000 / Gary Peter, John Cairney, Gerald Pullman -- Slide Material

Annual program review, forest biology, March 26-27, 1998

"March 26-27, 1998."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival ... Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers-embryo development improvements due to modification of plate size and wrapping materials ... Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: the effects of increasing ABA concentration and extending culture time on quality and quantity of cotyledonary somatic embryos of loblolly pine / Gerald Pullman and Xiaorong Feng ; Mass clonal propagation of improved conifers: optimizing plating methods for somatic embryo yields and automated embryo counting / Gary Peter, Teresa Vales ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of softwoods: functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of softwoods: update - functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers: the role of the suspensor in early embryo development. I. Differential gene expression in the suspensor of zygotic embryos / John Mackay, Heidi Schindler, Jerry Pullman ; Externally funded research in 1997-1998 supporting F010 / John Cairney ... [et al.] ; Gene expression in loblolly pine embryos: cDNA cloning, high density array and gene expression detection / Nanfei Xu ... [et al.] ; Isolation and characterization of "pinine", a vicilline life seed storage protein from loblolly pine (pinus taeda) / Ranjan Perera ... [et al.] ; Culture cycling and tissue culture decline: pH cycling in loblolly pine suspension cultures and associated gene expression / Michael Sullivan ... [et al.] ; cDNA cloning of mRNAs present early in zygotic embryo development / Vincent Ciavatta, Gerald Pullman and John Cairney ; Combined effects of activated carbon and pH on ionic composition and 2,4-D availability in a tissue culture medium / S.C. Van Winkle, G. S. Pullman ; Mass clonal propagation of genetically improved hardwoods: project F011 / Gary Peter ... [et al.] ; F011 mass clonal propagation of genetically improved hardwoods / Gary Peter ... [et al.] ; F011: mass clonal propagation of genetically improved hardwoods: establishing and improving the zinnia elegans tracheary element differentiation system at IPST / Gary Peter, Karen Floyd, Chris Ricker ; Externally funded research in 1997-1998 supporting F011 / John Cairney ... [ et al.] ; Gene expression in trees: sequences affecting stability/translatability of mRNA / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants: identification of a plant enzyme which activates a regulatory peptide / Luis Destefano ... [et al.]

Annual program review, forest biology

"March 25-26, 1999."Mass clonal propagation of improved conifers: status report for project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation / Gerald Pullman ... [et al.] ; Variation in initiation and the number of zygotic embryos in the seed / John MacKay ... [et al.] ; Survival of initiated cultures after direct transfer into liquid media: the effect of maltose, auxin type, and genotype / Gary Peter , Teresa Vales ; Embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman , Paul Montello , Xiaorong Feng ; Optimizing the percent of embryos that develop into cotyledonary embryos: the role of plating density and ABA concentration / Gary Peter , Teresa Vales ; Field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman , Paul Montello , Mike Cunningham ; Analysis of free amino acids, nitrate, and ammonium in zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Functional analysis of pinus taeda zygotic embryo germination: the effect of partial drying and the acquisition of desiccation tolerance / Gary Peter ... [et al.] ; Gene expression in pine embryos; developing tools / John Cairney , Gerald Pullman , John MacKay ; Differential gene expression in the suspensor of zygotic embryos / John MacKay ... [et al.] ; Analysis of a late stage-specific clone of loblolly pine by a non-radioactive method of cDNA labeling and detection / Barbara Johns and John Cairney ; Externally funded research in 1998-1999 supporting F010 / John Cairney ... [et al.] ; Developing reliable systems for evaluating gene expression using DNA arrays / Lin Ge , Jerry Pullman and John Cairney ; Early-specific gene expression in loblolly pine zygotic and somatic embryos: cDNS cloning, sequencing and expression analysis / Vincent Ciavatta , Gerald Pullman , John Cairney ; Fundamental biological mechanisms: improved stem growth rates and fiber properties: status reports for project F011 / Gary Peter ... [et al.] ; Externally funded research in 1998-1999 supporting F011 / Gary Peter ... [et al.] ; Transformation methods for southern pine: building an efficient transformation method for loblolly pine / Gary Peter ... [et al.] ; Environmental and genetic regulation of microfibril angle in southern pine: determination of the microfibril angle in the s2 layer of pinus taeda using differential interference contrast microscopy: validation of the method / Douglas Benton , Keith Bennett , Gary Peter ; Trees containing built-in pulping catalysts / Jerry Pullman ... [et al.] ; Lignin reactivity during pulping and bleaching of CAD-deficient pine / John MacKay ... [et al.] ; IPST forest biology research proposals ; Student research -- Slide Material

Annual program review, forest biology, October 14-15, 1998

"October 14-15, 1998."Mass clonal propagation of improved conifers: project F010 / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: genetic control of initiation and the number of zygotic embryos in the seed / John MacKay ... [et al.] ; Mass clonal propagation of improved conifers: survival of initiated cultures after direct transfer to liquid media / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman, Paul Montello, Xiaorong Feng ; Mass clonal propagation of improved conifers: optimizing the percent of embryos that develop into cotyledonary embryos by altering embryo plating densities and ABA concentration / Teresa Vales, Gary Peter ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Mass clonal propagation of improved conifers: functional anaylsis of pinus taeda zygotic embryo germination: the effect of partial drying and the acquisition of desiccation tolerance / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers: differential gene expression in the suspensor of zygotic embryos - update / John MacKay ... [et al.] ; Externally funded research in 1998-1999 supporting F010 / John Cairney ... [et al.] ; Gene expression studies with developing embryos: the effect of ABA treatment / Nanfei Xu ... [et al.] ; Early-stage-specific gene expression in loblolly pine zygotic and somatic embryos: cDNA cloning, sequencing and expression analysis / Vincent Ciavatta, Gerald Pullman and John Cairney ; Identification and cloning of cDNAs for novel female-, male- and vegetatively expressed genes / Lin Ge, Gerald Pullman, and John Cairney ; Fundamental biological mechanisms: improved stem growth rates and fiber properties: project F011 / Gary Peter ... [et al.] ; Fundamental biological mechanisms: improved fiber properties and rates of wood cell formation: cellulose biosynthesis / Gary Peter, Chris Ricker ; Externally funded reserach in 1998-1999 supporting F011 / Gary Peter ... [et al.] ; Towards genetic engineering of forest trees with anthraquinones: endogenous anthraquinones in pulpwood trees and the cloning of a key gene for anthraquinone biosynthesis / Huabin Meng ... [et al.] ; Environmental and genetic regulation of microfibril angle in southern pine: determination of the microfibril angle in the S2 layer of pinus taeda using differential interference contrast microscopy: validation of the method / Douglas Benton, Keith Bennett, Gary Peter ; Genetically modified lignin in pine: structure and properties: lignin reactivity in CAD-deficient trees during pulping and bleaching / Donald Dimmel ... [et al.] -- Slide material