L’intermède guadeloupéen de Georges Rohner : l’ensemble de la mairie de Basse-Terre (1934-1936)

Still an unknown painter, Rohner did his military service in Guadeloupe where he was commissioned by the town of Basse-Terre to execute a major work of six paintings destined to decorate the council chamber of the town hall and celebrating the three hundredth anniversary of the attachment of the French West Indies to France

Comparison of different vehicles to study the effect of tocopherols on gene expression in intestinal cells

International audienceRecent studies have focused on the ability of tocopherols to regulate gene expression. For such experiments, the methodology used to deliver molecules to the cells is crucial and could lead to different results depending on the vehicle used. The objective of the present study was to compare commonly used tocopherol vehicles (ethanol, BSA and mixed micelles) in terms of toxicity, stabilization of tocopherols, uptake efficiency of tocopherols by cells and effect on gene expression. Lactate dehydrogenase measurements did not reveal cytotoxicity of any of the tested vehicles. Tocopherol recovery measurements showed that ∼80% of the tocopherol was lost in ethanolic solutions, while only ∼30% and 10% were lost in BSA and mixed micelles, respectively. After 24 h incubation, Caco-2 cell monolayers treated with mixed micelles exhibited the highest agr-tocopherol intracellular concentrations (5-times those measured with the two other vehicles). Similar results were obtained with γ-tocopherol. Vehicles, except mixed micelles that activate the FXR/bile acids signalling pathway, did not affect expression of nuclear receptors involved in lipid metabolism or their target genes. This study establishes mixed micelles as the best vehicle to deliver tocopherols to intestinal cell monolayers in cultur

La constitution des autorités numériques dans la production et la circulation de l’information

Ce dossier ouvre la complexité de la notion d’autorité aux sciences de l’information et de la communication. Ce concept est utilisépar notre discipline, essentiellementpour étudierl'accès et le partage de l'information, l’organisation des connaissances, la communication organisationnelle et les travaux sur l’e-réputation. Les travaux sélectionnés et publiés dans ce numéro remettent en perspective les liens entre légitimité et autorité, reconnaissance et signatures énonciatives, visibilité et réputation à l’heure des plateformes du web. Les terrains d’investigation représentés se situent dans la communication culturelle, politique et médiatique ainsi que dans celui du document numérique