Purification and characterization of recombinant brazzein secreted by the yeast Pichia pastoris

National audienc

Purification and characterization of recombinant brazzein secreted by the yeast Pichia pastoris

Meeting AbstractInternational audienc

LAGoPEDE, a French breeding project to develop frost tolerant and FEVITA® <em>faba bean</em> varieties

National audienceThe INRA Plant Biology and Breeding division works together with the breeding company Agri Obtentions under the LAGoPEDE project to develop performant grain legume varieties for tomorrow’s agriculture. Grain legumes represent an important source of proteins for food and feed. One of the main targets of LAGoPEDE is to increase the area of cultivation and improve the seed quality of faba bean (Vicia faba) in France, by developing performant winter varieties well adapted to the North-East of the country. A frost tolerance screening of a diverse germplasm collection of 1,500 faba bean accessions available at INRA, Dijon was first conducted. Eleven frost-tolerant accessions were selected as genitors in LAGoPEDE. Considering the objective of producing FEVITA varieties, i.e. seeds with zero tannin and low vicine-convicine content, crosses between frost genitors and Mandoline, a FEVITA spring cultivar were performed. Additional crosses were made with genotypes exhibiting partial levels of tolerance to seed beetles (Coleoptera, Bruchinae) [1], an increasing problem for faba bean production. Phenotypic genealogical selection has essentially been undertaken for frost tolerance. Marker-assisted selection has been used to select low vicine-convicine genotypes [2]. LAGoPEDE will soon benefit of the outcomes of the PeaMUST project to include the SNP markers linked to quantitative trait loci responsible for bruchid and frost tolerance in the selection process

Modification des préférences gustatives et de la plasticité structurale des glandes salivaires par une exposition chronique au bisphenol A chez le rat male adulte

Modification des préférences gustatives et de la plasticité structurale des glandes salivaires par une exposition chronique au bisphenol A chez le rat male adulte. colloque de la SFOR

Optimization of the production of recombinant brazzein secreted by the yeast pichia pastoris

International audienc

Evidence for interactions between aroma compounds and the CB1 receptor: a way to regulate food intake?

National audienc

Système endocannoïde et molécules odorantes : une nouvelle piste pour réguler la prise alimentaire ?

Système endocannoïde et molécules odorantes : une nouvelle piste pour réguler la prise alimentaire ?. 30. réunion de l’association française d’étude et de recherche sur l’Obésite (AFERO

The pan-transcriptome of <em>Vicia faba</em>

National audienceVicia faba (faba bean) is a nitrogen-fixing protein crop that, in contrast to soya bean, yields well in temperate climates. It is diploid but has a very large genome of ~13 Gb, and genomic resources are currently limited. To provide a high-quality reference gene set and expression atlas, we have carried out RNA-seq of multiple tissues from five faba bean accessions. For each tissue, an assembly was produced using the Trinity software package using both Illumina and PacBio reads. For each accession, the assemblies were then merged to create a more complete reference gene set. The assemblies were of high quality, with more than 95% complete BUSCOs [1] and read mapping rates of ~90%. The assemblies for each accession were then joined to create a comprehensive reference gene set along with an expression atlas describing gene expression patterns across accessions and tissues. These resources will now facilitate gene discovery and characterisation in faba bean and speed up identification of genes associated with important traits including pest and disease resistance and antinutrients such as vicine and convicine

The faba bean pan-transcriptome

International audienceFaba bean (Vicia faba) is a widely adapted nitrogen fixing grain legume that produces seeds with high protein content. It is an agriculturally attractive crop with a good disease resistance profile, but targeted breeding is hampered by the lack of a reference genome and gene set. Here we develop a faba bean gene expression atlas and pan-transcriptome. The pan-transcriptome was generated using data from four different accessions (Hedin, Hiverna, 153b, 2378), including data from both aerial and root tissues. The quality and utility of the pan-transcriptome was assessed by mapping reads from accessions that had not contributed data to the pan-transcriptome. Here, the high mapping rates suggested that the pan-transcriptome represents a comprehensive faba bean reference gene set and that it will be a useful resource for differential gene expression analyses and genome annotation. In addition, the gene expression atlas provides valuable information to support gene function discovery