Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assays

A thorough understanding of the immune system, including the role of different cytokines, during inflammatory diseases in ruminants could lead to the development of new diagnostic methods and treatments. Tumour necrosis factor-α (TNF-α) is an important cytokine in the onset of the inflammatory responses. Unfortunately, the number of studies on cytokines, like TNF-α, in ruminants is limited due to a lack of species-specific reagents. As cytokines have remained rather conserved during evolution, cross-reactivity between animal species may occur. Therefore, the aim of the present study was to investigate 5 commercially available antibodies against human TNF-α for their ability to cross-react with ovine and/or bovine TNF-α, using a bead-based flow cytometric method. Two of the antibody clones (Mab 11 and 6401.1111) showed cross reactivity with ovine recombinant TNF-α in concentrations above 2.5 ng/ml. However, none of the antibodies detected TNF-α in bovine milk, or serum containing known concentrations of bovine TNF-α, as earlier determined with ELISA. The results could be due to inability of the antibodies to cross-react between species, but quenching of the signal by matrix proteins might also have lowered the response

Comparative study of dielectric spectroscopy measurements and anayses of oil and paper samples from a transformer undergoing repair

New methods, based on characterisation of dielectric response measurements, for assessing moisture content of pressboard and paper are gaining ground. However, there is a need for calibrating results with results from other techniques. This communication presents results from a study of a 19.5/6.8/6.8 kV - 40 MVA power transformer, which was opened on-site during maintenance work, thus allowing for collecting paper samples. Dielectric spectroscopy measurements were conducted before and after its opening. Results were compared with measurements on oil and paper samples. These showed that the moisture levels in the interior of the solid insulation were unchanged. Further, it was found that FDS measurements gave more accurate estimates of moisture levels than estimates based on oil analyses and equilibrium curves

Comparative study of dielectric spectroscopy measurements and anayses of oil and paper samples from a transformer undergoing repair

New methods, based on characterisation of dielectric response measurements, for assessing moisture content of pressboard and paper are gaining ground. However, there is a need for calibrating results with results from other techniques. This communication presents results from a study of a 19.5/6.8/6.8 kV - 40 MVA power transformer, which was opened on-site during maintenance work, thus allowing for collecting paper samples. Dielectric spectroscopy measurements were conducted before and after its opening. Results were compared with measurements on oil and paper samples. These showed that the moisture levels in the interior of the solid insulation were unchanged. Further, it was found that FDS measurements gave more accurate estimates of moisture levels than estimates based on oil analyses and equilibrium curves

Sequential Comparative Study of Dielectric Response and Analyses of Oil and Paper from a Power Transformer Undergoing Repair

Methods, based on characterisation of dielectric response, for assessing moisture content in pressboard and paper are gaining ground. However, there is a need for calibrating obtained results with results from other techniques. This communication presents results from a study of a 50/10 kV - 40 MVA power transformer, which was sent to a workshop for repair, thus allowing for collecting samples of the solid insulation. Dielectric spectroscopy measurements in frequency domain were conducted at several occasions before and after the repair. Moisture estimation based on dielectric response measurements as well as on titration of oil samples yielded similar results to those obtained by direct titration of paper samples, as long as appropriate temperature values were used in all the analyses mentioned. The temperature value during measurements should be used for the analysis of the dielectric response, while in-service temperature of oil should be used for the evaluation based on Karl Fisher titration