1 research outputs found
Exploiting Highly Ordered Subnanoliter Volume Microcapillaries as Microtools for the Analysis of Antibody Producing Cells
The interrogation of highly diverse
repertoires of heterogeneous
cell populations on a single cell basis increases the likelihood that
a cell with unique characteristics will be identified. We have developed
a new single cell analysis system comprising millions of bundled subnanoliter
volume bioincubation chambers for the identification and recovery
of target specific antibody secreting cells (ASCs). This platform
integrates dual surface screening with dedicated user driven data
analysis and automated cell recovery enabling multiple biophysical
parameters to be tracked for millions of antibody leads in parallel.
This direct clone analysis and selection technology is a clear deviation
from current microfabricated well-based approaches and offers drastically
enhanced screening throughput, simultaneous dual surface analysis,
and rapid automated single cell recovery. The technology is also applicable
to screening both bacterial and mammalian antibody secreting cells.
We demonstrate the implementation and feasibility of this platform
in identifying target specific antibodies from bacterial, hybridoma,
and B cell libraries