VASCULAR FLORA AND PLANT COMMUNITIES OF ALLEGHANY COUNTY, NORTH CAROLINA

Volume: 7Start Page: 529End Page: 57

SILENE FLOS-CUCULI SSP. FLOS-CUCULI (CARYOPHYLLACEAE) AND EUPHORBIA PEPLUS (EUPHORBIACEAE) NEW TO NORTH CAROLINA

Volume: 2Start Page: 649End Page: 65

A NEW HEDGE-NETTLE (STACHYS: LAMIACEAE) FROM THE SOUTHERN APPALACHIAN MOUNTAINS

Volume: 5Start Page: 405End Page: 41

A MORPHOLOGICALLY BASED TAXONOMIC REEVALUATION OF THE GENUS STIPULICIDA (CARYOPHYLLACEAE), WITH COMMENTS ON RANK

Volume: 8Start Page: 419End Page: 43

A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.

PremiseCounting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor.Methods and resultsHere we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos.ConclusionsThe protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources