Top annotated transcripts differentially expressed in resistant oysters at early time points after challenge with <i>R. crassostreae</i> (GX 1 and 5 days).

<p>Magnitude of differential expression in each treatment group is expressed as log₁₀ fold change (logFC) over expression in the control group (CGX15/30d). The name and accession number for the best BLASTx hits are shown.</p

Principal components (PC) analysis of gene expression in resistant and susceptible oysters experimentally challenged with <i>Roseovarius crassostreae:</i> Spatial projection of PC1 and PC2.

<p>The Z-score centered log2-transformed RPKM for each transcript in challenged susceptible oysters at days 1, 5, 15, and 30 after challenge (F3L_1 to F3L_30), challenged resistant oysters at days 1 to 30 (GX_1 to GX_30), and unchallenged resistant oysters at days 15 and 30 (CGX_15, CGX_30) was used in the PCA. Data from unchallenged resistant oysters at days 1 and 5 were not included in the analysis due to the potential confounding effect of an unrelated mortality event observed before day 7. Gene expression in unchallenged susceptible oysters (CF3L) was not studied.</p

Annotated transcripts differentially expressed in both resistant (GX_early) and susceptible (F3L early and/or late) oysters in response to challenge with <i>R. crassostreae</i>.

<p>Contig number, direction of differential expression (up- or down-regulation), and name and accession number for the best BLASTx hits are shown.</p

Heatmap of differentially expressed transcripts in resistant and susceptible oysters experimentally challenged with <i>Roseovarius crassostreae</i>.

<p>The Z-score centered log2-transformed RPKM for each transcript in each of eight sample groups is shown using a color scale. Genes are hierarchically clustered using Euclidean distance and complete linkage of the Z-score-transformed gene expression. Sample groups are clustered using the complete linkage Euclidean distance of the Spearman correlation of the Z-score-transformed gene expression.</p

Mortality in resistant and susceptible oysters after challenge with <i>Roseovarius crassostreae</i>.

<p>Cumulative percent mortality in resistant GX and susceptible F3L oysters following bacterial challenge compared to mortality in non-challenged (CGX, CF3L) oysters. Arrows on the x-axis indicate the timepoints at which RNA was isolated for RNA-seq.</p

Differentially expressed transcripts in response to bacterial challenge shared and unique between resistant and susceptible oysters.

<p>Venn diagram of shared and unique differentiall expressed transcripts in GX_early (resistant family–days 1 and 5), F3L_early (susceptible – days 1 and 5), and F3L_late (susceptible – days 15 and 30) oysters after challenge with the bacterial pathogen <i>Roseovarius crassostreae</i>.</p

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