A new glycoprotein SPG-8700 isolated from sweet potato with potential anti-cancer activity against colon cancer

<p>A new small molecule glycoprotein SPG-8700 with potential anti-colorectal cancer activity was firstly separated by tracking of bioactivity from a new sweet potato variety Zhongshu-1. Matrix-assisted laser desorption ionization mass spectrometry, high-performance liquid chromatography and amino acid analyzer were applied separately to determine the molecular weight and compositions of this glycoprotein. Flow cytometry analysis and western blotting analysis were employed to explore it’s mechanism of the anti-colorectal cancer. The molecular weight of glycoprotein was 8703.8D (SPG-8700). Relative sugar and protein contents in SPG-8700 were 73.4 and 26.6%, comprising more than 6 types of sugars (mannose, rhamnose, glucuronic acid, glucose, galactose and arabinose with a proportion of 1:6.9:7.3:1.5:46:21). Further results indicated that SPG-8700 promoted apoptosis in HCT-116 cells through regulating the expression of Bcl-2 and Bax and had no effect on the growth of normal cells.</p

GSTP1 Ile105Val Polymorphism and Prostate Cancer Risk: Evidence from a Meta-Analysis

<div><p>Background</p><p>Glutathione S-transferase P1 (GSTP1) is thought to be involved in the detoxification of reactive carcinogen metabolites. Numerous epidemiological studies have evaluated the association of GSTP1 Ile105Val polymorphism with the risk of prostate cancer. However, the results remain inconclusive. To derive a more precise estimation, a meta-analysis was performed.</p><p>Methodology/Principal Findings</p><p>A comprehensive search was conducted to identify the eligible studies. We used odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of the relationship. The overall association was not significant (Val/Val vs. Ile/Ile OR = 1.06, 95% CI = 0.90–1.25, <i>P</i> = 0.50; Val/Val vs. Val/Ile+Ile/Ile: OR = 1.07, 95% CI = 0.91–1.25, <i>P</i> = 0.44). In subgroup analyses by ethnicity and prostate cancer grade, the similar results were observed. However, in stratified analysis by clinical stage, we found a significant association with low-stage prostate cancer (Val/Val vs. Ile/Ile: OR = 2.70, 95% CI = 1.73–4.22, <i>P</i><0.001; Val/Val vs. Val/Ile+Ile/Ile: OR = 2.14, 95% CI = 1.38–3.33, <i>P</i> = 0.001). Moreover, there was no statistically significant evidence of multiplicative interactions neither between the GSTP1 Ile105Val polymorphism and GSTM1, nor between smoking status and GSTP1 on prostate cancer risk.</p><p>Conclusions</p><p>This meta-analysis showed that GSTP1 Ile105Val polymorphism might not be significantly associated with overall prostate cancer risk. Further stratified analyses showed a significant association with low-stage prostate cancer.</p></div

Flow chart of study selection based on the inclusion and exclusion criteria.

<p>Flow chart of study selection based on the inclusion and exclusion criteria.</p

Begg’s funnel plots for publication bias test on studies assessing GSTP1 Ile105Val polymorphism and prostate cancer risk (A, Val/Val vs. Ile/Ile; B, Val/Ile vs. Ile/Ile; C, Val/Val vs. Val/Ile+Ile/Ile; D, Val/Val+Val/Ile vs. Ile/Ile).

<p>Each circle represents as an independent study for the indicated association.</p

Begg’s funnel plot for publication bias test [for Null versus Present].

<p>Each point represents a separate study for the indicated association. Log[or], natural logarithm of OR. Horizontal line, mean effect size.</p

Forest plot of PCa risk associated with GSTM1 null polymorphism (for Null versus Present).

<p>The squares and horizontal lines correspond to the study-specific OR and 95% CI. The area of the squares reflects the weight (inverse of the variance). The diamonds represent the summary OR and 95% CI.</p

Forest plot of PCa risk associated with GSTM1 null polymorphism among Asians (for Null versus Present).

<p>The squares and horizontal lines correspond to the study-specific OR and 95% CI. The area of the squares reflects the weight (inverse of the variance). The diamonds represent the summary OR and 95% CI.</p